Effects of glycerol concentration and pH on growth of recombinantPichia pastoris yeast

Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris

“…Although the host and vector system and a fed-batch fermentation process have been developed for the Mut -strain [7,23], the use of Mut − strain of P. pastoris as an expression host has been limited. After transforming the Mut − strain with the appropriate expression vector, screening for the best expression clone is difficult because the common sources of carbon, e.g.…”

“…Previous studies [7,23,28] revealed that during the batch, fed-batch, or induction phase, the use of glycerol results in ethanol production. Ethanol repression of the AOX1 promoter was investigated using the GS115 (Mut + ) host, expressing intracellular β-galactosidase.…”

“…The strategies to run GBP and GFP are the same for all three phenotypes of strains: Mut + , Mut s and Mut − since their growth on glycerol are similar [23]. A cell density of about 100 g/L WCW can be generated at the end of GBP when employing BSM+0.435% PTM1 medium with 40 g/L of glycerol.…”

“…A glycerol concentration over 40 g/L could inhibit growth in GBP [33]. Chiruvolu et al [23] determined that a 0.5-2.4% level of ethanol was produced when the initial glycerol level was over 7%, and Brierley [31] recommends a maximum of 6%.…”

Produção de fragmento recombinante de anticorpo em Pichia pastoris

“…A P. pastoris é uma célula eucariota robusta e assim como outras leveduras pode ser cultivada em uma faixa de pH de 3,0 a 8,0 sem grandes efeitos sobre a viabilidade e o crescimento celular (Chiruvolu et al, 1998;Cereghino e Cregg, 2000).…”