Effects of glycerol on enzymatic hydrolysis and ethanol production using sugarcane bagasse pretreated by acidified glycerol solution

Zhang, Zhanying; Wong, Heng Ho; Albertson, Peter; Harrison, Mark D.; Doherty, William O.S.; O’Hara, Ian M.

doi:10.1016/j.biortech.2015.05.093

Cited by 43 publications

(23 citation statements)

References 28 publications

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“…3a, c). A previous study indicated that enzymatic hydrolysis was slightly inhibited with 0.2% (w/v) glycerol [44]. According to the current results, the glycerol content was below 0.2% (w/v).…”

Section: Resultssupporting

confidence: 66%

“…Glycerol is obtained under osmotic pressure changes and low oxidation–reduction potential. It is also possible that glycerol acts as a glucose analog, resulting in end-product inhibition of cellulase activity [44, 45]. Therefore, it is important to study the production of glycerol in the ethanol fermentation process.…”

Section: Resultsmentioning

confidence: 99%

“…According to the current results, the glycerol content was below 0.2% (w/v). Besides, glycerol did not irreversibly inhibit cellulase enzymes [44]. Evidently, more studies are needed to find the concentration at which glycerol inhibits cellulase enzyme.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of ethanol production from green liquor–ethanol-pretreated sugarcane bagasse by glucose–xylose cofermentation at high solid loadings with mixed Saccharomyces cerevisiae strains

You

Lei

et al. 2017

Biotechnol Biofuels

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BackgroundEfficient cofermentation of glucose and xylose is necessary for economically feasible bioethanol production from lignocellulosic biomass. Here, we demonstrate pretreatment of sugarcane bagasse (SCB) with green liquor (GL) combined with ethanol (GL–Ethanol) by adding different GL amounts. The common Saccharomyces cerevisiae (CSC) and thermophilic S. cerevisiae (TSC) strains were used and different yeast cell mass ratios (CSC to TSC) were compared. The simultaneous saccharification and cofermentation (SSF/SSCF) process was performed by 5–20% (w/v) dry substrate (DS) solid loadings to determine optimal conditions for the co-consumption of glucose and xylose.ResultsCompared to previous studies that tested fermentation of glucose using only the CSC, we obtained higher ethanol yield and concentration (92.80% and 23.22 g/L) with 1.5 mL GL/g-DS GL–Ethanol-pretreated SCB at 5% (w/v) solid loading and a CSC-to-TSC yeast cell mass ratio of 1:2 (w/w). Using 10% (w/v) solid loading under the same conditions, the ethanol concentration increased to 42.53 g/L but the ethanol yield decreased to 84.99%. In addition, an increase in the solid loading up to a certain point led to an increase in the ethanol concentration from 1.5 mL GL/g-DS-pretreated SCB. The highest ethanol concentration (68.24 g/L) was obtained with 15% (w/v) solid loading, using a CSC-to-TSC yeast cell mass ratio of 1:3 (w/w).ConclusionsGL–Ethanol pretreatment is a promising pretreatment method for improving both glucan and xylan conversion efficiencies of SCB. There was a competitive relationship between the two yeast strains, and the glucose and xylose utilization ability of the TSC was better than that of the CSC. Ethanol concentration was obviously increased at high solid loading, but the yield decreased as a result of an increase in the viscosity and inhibitor levels in the fermentation system. Finally, the SSCF of GL–Ethanol-pretreated SCB with mixed S. cerevisiae strains increased ethanol concentration and was an effective conversion process for ethanol production at high solid loading.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of ethanol production from green liquor–ethanol-pretreated sugarcane bagasse by glucose–xylose cofermentation at high solid loadings with mixed Saccharomyces cerevisiae strains

You

Lei

et al. 2017

Biotechnol Biofuels

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“…One merit of using polyhydroxy alcohols is that the process can be performed under atmospheric pressure. Moreover, glycerol usually can be utilized by microorganisms as a carbon source and it would not show significant inhibition in case that some glycerol remains in the enzymatic hydrolyzate . Sulfuric acid is usually used to catalyze the delignification process.…”

Section: Acid‐catalyzed Process Alcohol Pre‐treatment and Mechanismsmentioning

confidence: 99%

Organosolv fractionating pre‐treatment of lignocellulosic biomass for efficient enzymatic saccharification: chemistry, kinetics, and substrate structures

Zhao¹,

et al. 2017

Biofuels Bioprod Bioref

203

136

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BUSINESS HIGHLIGHTS 403 Crop-cap warning | Dear Mr. President | Biofuel research at NASA | The challenges of seaweed | Ethanol plant for sale | BP to acquire biomethane production facility | Anti-dumping petition TECHNOLOGY NEWS 406 Mustard plants could make tasty new biodiesel feedstock | Nuts to biofuels and batteries PEER REVIEWED PERSPECTIVE 407 Life cycle assessment is the most relevant framework to evaluate biofuel greenhouse gas burdens MODELING AND ANALYSIS 417 Techno-economic assessment of biomass bales storage systems for a large-scale biorefi nery Kamalakanta Sahoo, Sudhagar Mani 430 Corn stover as feedstock for the production of ethanol: chemical composition of different anatomical fractions and varieties 454 Planning the optimal site, size, and feed of biogas plants in agricultural districts Demetrio Antonio Zema 472 Techno-economic assessment of biogas to liquid fuels conversion technology via Fischer-Tropsch synthesis Ikenna J. Okeke, Sudhagar Mani 488 Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production REVIEWS 521 EliteTree TM : an advanced biomass tree crop technology that features greater wood density and accelerated stem growth The cover image, by Xuebing Zhao et al., is based on the Review Organosolv fractionating pre-treatment of lignocellulosic biomass for effi cient enzymatic saccharifi cation: chemistry, kinetics, and substrate structures,

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“…Previous study has reported that glycerol has potential as a delignification agent in the presence of alkaline substances [7]. Zhang et al has reported that glycerol thermal processing can disrupt the cell wall without noticeable change in chemical composition [8].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Steam and Glycerol Pretreatment on Chemical Contents of Oil Palm Empty Fruit Bunch (EFB)

et al. 2018

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This research aimed to evaluate the effect of the type of solvent, pH, substrate loading, and reaction time on the chemical components of palm empty fruit bunches (EFB). Steam pretreatment was set up at a temperature of 121 °C and pressure of 1.18 bar, using an autoclave with substrate loading of 5, 10, 15 and 20 % w/v at reaction times of 15 and 60 min. Distilled water, waste glycerol, alkaline glycerol and acidic glycerol were compared as solvents during steam pretreatment. The results showed that with distilled water, better pretreatment was achieved at 5 % and 10 % loading for 60 min. During the pretreatment with waste glycerol at 5 % loading an increase on the reaction time from 15 to 60 min reaction resulted in a remarkable increase in reducing sugar in the liquid phase. Overall, the best steam pretreatment conditions were observed using alkaline glycerol at 5 % w/v and 15 min reaction time, resulting in holocellulose (cellulose plus hemicellulose) increase to 87.98 % and a lignin decrease to 9.17 %. However, pretreatment with glycerol for 15 min was better than those for 60 min using either glycerol or distilled water. The results suggest that waste glycerol during steam pretreatment of EFB can be utilized effectively at short reaction times and at an increased pH to achieve a high output of cellulose and hemicellulose for sugar conversion in the bioethanol fermentation process.

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Effects of glycerol on enzymatic hydrolysis and ethanol production using sugarcane bagasse pretreated by acidified glycerol solution

Cited by 43 publications

References 28 publications

Enhancement of ethanol production from green liquor–ethanol-pretreated sugarcane bagasse by glucose–xylose cofermentation at high solid loadings with mixed Saccharomyces cerevisiae strains

Enhancement of ethanol production from green liquor–ethanol-pretreated sugarcane bagasse by glucose–xylose cofermentation at high solid loadings with mixed Saccharomyces cerevisiae strains

Organosolv fractionating pre‐treatment of lignocellulosic biomass for efficient enzymatic saccharification: chemistry, kinetics, and substrate structures

The Effect of Steam and Glycerol Pretreatment on Chemical Contents of Oil Palm Empty Fruit Bunch (EFB)

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