Effects of glyphosate on the movement of assimilates of two Lolium perenne L. populations with differential herbicide sensitivity

Yanniccari, Marcos; Istilart, Carolina María; Giménez, Daniel; Castro, Ana María

doi:10.1016/j.envexpbot.2012.03.006

Cited by 22 publications

(19 citation statements)

References 29 publications

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“…Reduced absorption is a mechanism not usually involved in the glyphosate resistance and has been described in a few species such as D. insularis, L. multiflorum , and S. halepense (Michitte et al, 2007; de Carvalho et al, 2012; Vila-Aiub et al, 2012). Yanniccari et al (2012) suggested that there is an unidentified barrier that prevents glyphosate to load into the phloem. In the resistant L. virgata populations, the reduced glyphosate absorption played an important role, mainly in the first 24 HAT, due to a clear reduction compared to the S population.…”

Section: Discussionmentioning

confidence: 99%

“…Greater movement of glyphosate to the meristematic tissues is crucial for plant mortality (Adu-Yeboah et al, 2014). However, reduced glyphosate translocation in resistant plants is mostly retained within the treated leaves (Yanniccari et al, 2012). Plants of the resistant L. virgata populations showed low glyphosate translocation to the meristematic tissues of the roots.…”

Section: Discussionmentioning

confidence: 99%

“…Reduced glyphosate translocation is due to a trait (unknown barrier) that restricts glyphosate movement within resistant plants (Powles, 2010), or an altered subcellular distribution of glyphosate, keeping it away from the target-site (Kleinman and Rubin, 2016). The barrier may exist either in the phloem system or in the mesophyll cells, where glyphosate must enter to be translocated (Yanniccari et al, 2012). Glyphosate resistance by this mechanism confer by a single nuclear gene with complete- or semi-dominance (Shaner, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Some are: limited glyphosate leaf absorption (Vila-Aiub et al, 2012); reduced glyphosate translocation to meristematic zones, mainly restricted within the treated leaves (Shaner, 2009; Yanniccari et al, 2012); sequestration of glyphosate in the vacuole (Ge et al, 2012); and glyphosate degradation to non-toxic compounds (sarcosine, glyoxylate, formaldehyde and amino methyl phosphonate) (Duke, 2011) resulting in significantly less glyphosate throughout the whole plants.…”

Section: Introductionmentioning

confidence: 99%

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First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

et al. 2016

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Leptochloa virgata (L.) P. Beauv. is an annual weed common in citrus groves in the states of Puebla and Veracruz, Mexico limiting their production. Since 2010, several L. virgata populations were identified as being resistant to glyphosate, but studies of their resistance mechanisms developed by this species have been conducted. In this work, three glyphosate-resistant populations (R8, R14, and R15) collected in citrus orchards from Mexico, were used to study their resistance mechanisms comparing them to one susceptible population (S). Dose-response and shikimic acid accumulation assays confirmed the glyphosate resistance of the three resistant populations. Higher doses of up to 720 g ae ha-1 (field dose) were needed to control by 50% plants of resistant populations. The S population absorbed between 7 and 13% more 14C-glyphosate than resistant ones, and translocated up to 32.2% of 14C-glyphosate to the roots at 96 h after treatment (HAT). The R8, R14, and R15 populations translocated only 24.5, 26.5, and 21.9%, respectively. The enzyme activity of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was not different in the S, R8 and R14 populations. The R15 Population exhibited 165.9 times greater EPSPS activity. Additionally, this population showed a higher EPSPS basal activity and a substitution in the codon 106 from Proline to Serine in the EPSPS protein sequence. EPSPS gene expression in the R15 population was similar to that of S population. In conclusion, the three resistant L. virgata populations show reduced absorption and translocation of 14C-glyphosate. Moreover, a mutation and an enhanced EPSPS basal activity at target-site level confers higher resistance to glyphosate. These results describe for the first time the glyphosate resistance mechanisms developed by resistant L. virgata populations of citrus orchards from Mexico.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

et al. 2016

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“…It also disrupts the de novo biosynthesis of most non-aromatic amino acids, coupled with decreased protein synthesis (Maroli et al, 2016). In consequence, growth is inhibited (Pline et al, 2002;Orcaray et al, 2012), the transport of assimilates is reduced (Yanniccari et al, 2012a) and photosynthetic CO 2 assimilation decreases significantly (Olesen and Cedergreen, 2010). Several of these effects are limited or absent in glyphosate-resistant weed plants treated with the herbicide (Yanniccari et al, 2012a,b,c;Maroli et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

et al. 2017

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In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.

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Photosynthetic Modulation in Response to Plant Activity and Environment

Adams

2018

Advances in Photosynthesis and Respiration

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Effects of glyphosate on the movement of assimilates of two Lolium perenne L. populations with differential herbicide sensitivity

Cited by 22 publications

References 29 publications

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

Photosynthetic Modulation in Response to Plant Activity and Environment

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