Effects of grafting on key photosynthetic enzymes and gene expression in the citrus cultivar Huangguogan

Liao, Ling; SuiZhong, Cao; Rong, Yujing; Wang, Z H

doi:10.4238/gmr.15017690

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…The number of fruit and biomass yield per plant were also recorded. Total soluble solids (TSS, %), total acid (TA, g/100 mL), and vitamin C (Vc, mg/100 mL), were measured after Liao et al [ 50 ]. Root activity was analyzed using triphenyl tetrazolium chloride (TTC) [ 51 ], and the soluble protein content was calculated as in He et al [ 52 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’

et al. 2019

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Nitrogen metabolism in citrus has received increased attention due to its effects on plant growth and productivity. However, little is known about the effects of nitrogen fertilization on nitrogen metabolism in young trees of citrus cultivar ‘Huangguogan’ (Citrus reticulata × Citrus sinensis). Here, genes encoding nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), and asparagine synthetase (AS), represented as HgNR, HgNiR, HgGS, HgGDH, and HgAS, respectively, were cloned from Huangguogan. Deduced protein sequences were analyzed and proteins were confirmed to be localized in their respective cellular organelles. Moreover, pot-cultured ‘Huangguogan’ seedlings were fertilized with 0 (N1), 1.36 (N2), 1.81 (N3), 2.26 (N4), or 2.72 (N5) kg N/year, for 12 months. Enzyme activity and enzyme-gene expression were studied in roots, leaves, and fruits at different stages. Finally, the effects of N application rate on root activity, leaf N, soluble protein, yield, and fruit quality at the ripening stage were measured. The results showed that: 1) HgNR, HgNiR, HgGDH, and HgAS gene products were found mainly in the cytoplasm and plasma membrane, while HgGS gene product was found mainly in cytoplasm and mitochondria. 2) Gene expression and enzyme activity differed among plant organs. As the root is in permanent direct contact with the soil we suggest that root gene expression and enzyme activity can be used as reference to determine N application rate. 3) Yield, fruit quality, enzyme activity, and enzyme-related gene expression were considerably lower at low than at high-N supply. However, they were all inhibited by excess nitrogen (i.e., 2.72 kg/year). Therefore, we recommend 1.81 kg N/year as the optimal N application rate for young ‘Huangguogan’ trees.

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Section: Methodsmentioning

confidence: 99%

Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’

et al. 2019

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“…Huangguogan has been cultivated for over 300 years in the local, trifoliate [Poncirus trifoliata (L.) Raf], tangerine (Citrus reticulata Blanco), and Ziyang Xiangcheng [Citrus junos (Sieb.) Tanaka] are used frequently in Huangguogan grafting (Liao et al, 2016). A good rootstock should be compatible with the scion cultivar, resistant and/or tolerant to pests and diseases, and adaptable to a wide range of soil types and climatic conditions (Hernández, Pinochet, Moreno, Martínez, & Legua, 2010).…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic model for citrus cultivar Huangguogan

Liao

Ronga

Qiua

et al. 2020

SCA

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Grafting is an effective measure to improve the photosynthetic rate of citrus. The light responses of photosynthesis in leaves of two-year old grafted Huangguogan (citrus cultivar Huangguogan), Huanggougan / Trifoliate (HG/PT), Huanggougan / Tangerine (HG/CR), and Huanggougan / Ziyang Xiangcheng (HG/CJ) were studied using the LI-COR 6400 portable photosynthesis system. Lightresponse curves and photosynthetic parameters were analyzed and fitted using the rectangular hyperbola model (RHM), the exponential model (EM), the non rectangular hyperbola model (NRHM), and the modified rectangular hyperbola model (MRHM). The results showed that: (1) Grafting can change the photosynthetic characteristics of Huangguogan, and the value of photosynthesis rate of HG/CJ is the greatest; (2) The light-response curves of net photosynthetic rate (PN), the light compensation point (LCP), and the dark respiration rate (RD) were well fitted using the above four models. The modified rectangular hyperbola was the best model in fitting the data; the nonrectangular hyperbola model was the second, and the rectangular hyperbola model was the poorest one.

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“…The canopy size, tree vigor of different ages 'Huangguogan' trees was basically the same. Stands of 8-year-old (8s), 30-year-old (30s), and 280-year-old (280s) 'Huangguogan' trees were maintained under the same conditions (i.e., fertilization, soil management, disease control, and pruning) as described by Liao et al (2016) more than five years. Each group had four replicates, so the total number of the experimental 'Huangguogan' plants was 12 (3 groups × 4 replicates), all the planting spacing was 4 × 5 m.…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of leaf photosynthetic characteristics and fruit sugar content in trees of Citrus cultivar 'Huangguogan' of different age

Xiong¹,

Qiu²,

Huang³

et al. 2020

Photosynt.

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Citrus trees of cv. Huangguogan, 8-, 30-, and 280-year-old (8s, 30s, and 280s, respectively), were used to explore the photosynthesis and related enzyme activity. In spring shoot leaves, net photosynthetic rate (PN) of the 280s was significantly lower than that of the 30s and 8s, but it was opposite in summer shoot leaves. PN of the summer shoots was higher than that of the spring shoots. PN was significantly positively correlated with stomatal conductance, intercellular CO2 concentration, and transpiration rate, but was negatively correlated with maximum photochemical quantum yield of PSII photochemistry. The effect of age on variable fluorescence/minimal fluorescence was similar to that of minimal fluorescence, variable fluorescence, maximal fluorescence yield of the dark-adapted state. Fruits of the 280s had significantly lower sugar components than those of the 8s and 30s. Results showed that fruits of the 8s and 30s actively accumulated photosynthesis products, which was verified by the higher PN in the spring shoots, lower acidic invertase and neutral invertase activities, and higher sucrose phosphate synthase than that of the 280s.

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Effects of grafting on key photosynthetic enzymes and gene expression in the citrus cultivar Huangguogan

Cited by 5 publications

References 35 publications

Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’

Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’

Photosynthetic model for citrus cultivar Huangguogan

Comparative analysis of leaf photosynthetic characteristics and fruit sugar content in trees of Citrus cultivar 'Huangguogan' of different age

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