Effects of granulocyte colony-stimulating factor on electrocardiogram changes after carbon monoxide poisoning in rats

Abstract

Carbon monoxide (CO), which is produced by the incomplete combustion of hydrocarbons, has many toxic effects on different organs, especially the brain and heart. CO-induced cardiotoxicity leads to several deleterious effects, including electrocardiogram (ECG) abnormalities. The present study aimed to evaluate the protective effect of recombinant human granulocyte colony-stimulation factor (G-CSF) on ECG after CO poisoning in rats. Single and multiple doses of G-CSF (10, 50, and 100 µg/kg) were administered to groups, each containing 5 male Wistar rats (16 groups for ECG analysis and 16 groups for pathological analysis). Rats were already exposed to CO at either 1,500 or 3,000 ppm concentrations for 60 minutes. ECG findings (e.g., ST-segment and T-wave changes), cardiac arrhythmias (e.g., heart blocks and ventricular and supraventricular arrhythmias), and histological changes were determined after G-CSF administration. At 3,000 ppm, frequencies of ST elevation, depression, and T inversion in ECG were significantly reduced after G-CSF treatment. Also, some of the cardiac arrhythmias (e.g., atrioventricular block type 1 and 2) after CO poisoning were suppressed after G-CSF treatment. However, G-CSF did not show protective effects on cardiomyocyte pathological consequences in CO-poisoned rats. Therefore, G-CSF could protect against ECG changes after CO-induced cardiac ischemia, but did not affect pathological changes.

Neuro‐Protective Effects of Resveratrol on Carbon Monoxide‐Induced Toxicity in Male Rats

“…In previous studies, it was shown that by measurement of COHb levels in the blood of the exposed animals, we can confirm the induction of CO poisoning (Ghorbani et al , ; Mohamadpour et al , ). Our results showed that exposure to CO 3000 ppm for 1 h leads to a COHb level of 75 ± 9 ppm which confirmed CO intoxication.…”

“…Our previous study showed that RES could ameliorate CO‐induced cardiovascular effects in an animal model (Hashemzaei et al , ). In order to find new substances that can fight the deleterious effects of CO poisoning in the heart and brain and also to shed light on the underlying mechanisms, in our previous studies, we discussed the involved pathways (Ghorbani et al , ; Hashemzaei et al , ; Mohamadpour et al , ). To the best of our knowledge, it is for the first time that the neuroprotective effect of multiple‐dose RES administration on CO poisoning is evaluated in rats.…”

“…In order to find new substances that can fight the deleterious effects of CO poisoning in the heart and brain and also to shed light on the underlying * Correspondence to: Mahmoud Hashemzaei, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. E-mail: mhtoxicologist@gmail.com mechanisms, in our previous studies, we discussed the involved pathways (Ghorbani et al, 2017;Hashemzaei et al, 2016b;Mohamadpour et al, 2012). To the best of our knowledge, it is for the first time that the neuroprotective effect of multiple-dose RES administration on CO poisoning is evaluated in rats.…”

Magnesium sulfate protects the heart against carbon monoxide-induced cardiotoxicity in rats

“…ECG in lead I was recorded using PowerLab (ADInstruments, Bella Vista, New South Wales, Australia) before, during, and after CO poisoning. The ECG was analyzed with special focus on ST segment, T wave, atrioventricular (AV) block type 1 and 2, Q wave, premature ventricular contraction (PVC), sick sinus syndrome, ventricular tachycardia and fibrillation and atrial fibrillation ( 7 ).…”

“…Despite considerable advances in poisoning management, CO is still the leading cause of unintentional poisoning worldwide ( 5 6 ). CO is a colorless, odorless, tasteless and non-irritating gas which exerts its deleterious effects mostly in the organs with high oxygen demand including the brain and heart ( 5 7 8 ). Despite advances in toxicology, CO poisoning management has not been markedly changed over the last 100 years and still employs normobaric and hyperbaric oxygen along with symptomatic therapy ( 7 ).…”