Effects of grape pomace and vitamin E on performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens

Abstract

We investigated the effects of grape pomace (GP) and vitamin E on the performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens. Two hundred and fifty male broiler chickens (Ross 308) were allocated to five dietary treatments (50 birds/treatment) in a completely randomized design. The experimental diets were as follows: i) Control corn-soybean meal diet (C); ii) C + Vitamin E (200 mg/kg of α-tocopherol acetate); iii) The diet containing 5%GP; iv) The diet containing 7.5% GP; v) The diet containing 10% GP. No differences were found in performance of the experimental birds. Birds fed 7.5% GP showed a significant increase in superoxide dismutase (SOD), glutathione peroxidise (GSH-Px) activity and reduced malondialdehyde (MDA) concentration. Antibody titer against Newcastle disease virus (NDV) at 42 days (d) of age in the control and 7.5% GP fed groups were lower than others. The primary IgM concentration of birds fed 10% GP was higher than the birds fed the control diet, the vitamin E supplemented diet or the diet containing 5% GP. The secondary titer against sheep red blood cells (SRBC) was increased significantly in the birds fed 10% GP and the secondary IgG concentration of birds fed 10% GP was higher than the birds fed the control diet and the diets containing 5% or 7.5% GP. In duodenum part of small intestine, villus height/crypt depth ratio and muscularis thickness were decreased. The results of the present study suggest that the inclusion of up to 10% GP in diets did not adversely affect broiler chickens' performance and improved the antioxidant and immune responses of broiler chickens. ______________________________________________________________________________________

Effect of Dietary Grape Seed Supplementation as a Natural Growth Promoter on the Growth Performance of Japanese Quail

“…Grape seed supplementation in the diet did not significantly affect blood plasma AST and ALT levels of broilers. Similarly, Ebrahimzadeh et al (2018) GP supplementation to chicken diets did not have any significant impact on serum glucose while decreased triglycerides, LDL and increased HDL concentration of serum blood of broilers. In this study, the hypolipidemic effects of GP might not originate from their direct effect on lipid metabolism; however, it could be mediated by their effect on lowering oxidized lipid products.…”

“…In this study, the hypolipidemic effects of GP might not originate from their direct effect on lipid metabolism; however, it could be mediated by their effect on lowering oxidized lipid products. This effect may be associated with an increase in the plasma HDL concentration of the respective treatment groups (Ebrahimzadeh et al, 2018). Either antioxidant parameters (GPx and TBAR) or immune responses significantly (P≤0.001) affected by treatment effect (Table 6).…”

“…Similarly, Fawzia et al (2014) indicated that GSP supplementation in the diet did not significantly affect all of carcass traits studied, except for pre-slaughter and carcass weight percentages, which were significantly higher in rabbits receiving 1.0% GSP. Recent results from Ebrahimzadeh et al (2018) showed that GP supplementation had no significant effect on carcass, abdominal fat, liver or heart weights in birds. Table 4 revealed that neither treatment nor sex significantly influenced all carcass chemical composition of quail meat, except ash%.…”

Effect of Using Grape Seeds on Productive Performance and Nutrients Utilization for Broiler Chicks during Fattening Period

“…Similarly, Aditya et al (2018) reported that carcass traits for commercial broilers were not significantly affected by GP addition (5, 7.5 and 10 g/kg) compared with the control. Dietary grape pomace (5, 7.5 and 10%) addition had no significant effect on relative weights of carcass, abdominal fat, liver and heart for broilers (Ebrahimzadeh et al, 2018). El-Kelawy et al (2018) found that natural sources of polyphenols supplementations to broiler chickens diet increased percentage of dressing and total edible parts compared with control.…”

Effect of Dietary Proanthocyanidin Supplementation on Productive Performance and Carcass Quality of Local Domyati Ducklings

“…Also, Farahat et al (2017) found that GSE can be used as an effective natural antioxidant and immune stimulating agent in broiler diets from 125 to 250 mg/kg diet. Recently, Ebrahimzadeh et al (2018) reported that dietary addition of grape pomace could increase the antioxidant and immune responses parameters of treated broilers. Regarding the blood biochemical parameters, Hajati et al (2014) indicated that addition of 450 mg GSE/kg to broiler diet decreased total cholesterols, triglycerides, LDL and vLDL.…”