Effects of grape seed-derived proanthocyanidin B2 pretreatment on oxidative stress, endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells in renal ischemia–reperfusion injury model of mice

Wang, Zhi-Shun; Shu, Bo; Han, Qianqian; Li, Guo-hao; Guo, Yonglian

doi:10.1007/s11255-023-03494-4

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…Thus, to clarify the therapeutic mechanism of PCs, we applied different-concentration PCs to interfere with the HSP-like cell model basing on the determination of drug safe concentrations and the establishment of cell model. Our outcomes exhibited that PCs greatly lowered the expressions of IgA, IL-4, IL-8, IL-17, TNF-α, NO, MDA and ROS, while markedly enhanced those of SOD, CAT and GSH, which was similar to the results from Wang and Chen, et al; 47 , 48 , 51–53 they discovered that PCs powerfully facilitated the decrease of inflammatory factors and oxidative substance, and the increase of antioxidants. Based on our experimental results, there does not appear to be a significant difference between the PCs groups at different concentrations when compared to the model group.…”

Section: Discussionsupporting

confidence: 89%

“…A serial of studies has shown that PCs actively exert in many diseases through inhibiting inflammatory factors production and arresting OS damage. [46][47][48][49] More importantly, our previous experiment tentatively uncovered that PCs could effectively alleviate cutaneous and renal injuries in HSP-like rats probably via suppression of inflammatory responses and OS insults, 50 but the exact mechanism of PCs against HSP still kept unclear. Thus, to clarify the therapeutic mechanism of PCs, we applied different-concentration PCs to interfere with the HSP-like cell model basing on the determination of drug safe concentrations and the establishment of cell model.…”

Section: Discussionmentioning

confidence: 98%

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Protection of Proanthocyanidins Against HSP Serum-Induced Inflammation and Oxidative Stress on Human Umbilical Vein Endothelial Cells

Liu,

Wang,

Guo

et al. 2024

CCID

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Background Immune-mediated inflammation and oxidative stress play pivotal roles in Henoch-Schonlein purpura (HSP), primarily through the TLR4/MyD88/NF-κB pathway. Proanthocyanidins (PCs) exert anti-inflammatory and antioxidant effects by regulating some signals like TLR4/MyD88/NF-κB. Previous research uncovered that PCs could alleviate purpura-like lesions and pathological changes on rats likely through attenuating inflammation and OS damage. The mechanism of PCs on HSP deserves further investigation. Objective To clarify the potential mechanism of PCs to HUVECs induced by the serum of HSP patients. Methods HUVECs were randomly divided into blank, control, model, and low-, medium-, and high-concentration PCs group. Then, 25% HSP serum was assigned to the latter four groups, while 25% serum from healthy subjects to control group and serum-free culture medium to blank one. The last three groups separately received different concentrations of PCs. In addition, TAK-242, a TLR4 inhibitor, was applied to investigate the effect of TLR4-related signals in PCs against HSP serum-induced damage. Finally, inflammatory and OS-related parameters were detected by using cytological/molecular-biological techniques. Results Treated with HSP serum later, the levels of immuno-inflammatory and oxidative indicators obviously went up (P < 0.05), and those of antioxidants remarkably went down (P < 0.05). PCs, however, reversed above phenomena (P < 0.05). Moreover, TLR4, MyD88 and NF-κB proteins/genes highly expressed in the model group; but significantly fell off in the presence of PCs (P < 0.05). Amazingly, all of above indicators showed no significant difference among the groups of different PCs concentrations (P > 0.05). These alterations likewise occurred after TAK-242 pretreatment with or without PCs, ie a notable drop of TLR4, MyD88 and NF-κB appeared in TAK-242 presence, few differences existing when compared to the PCs groups. Conclusion PCs effectively protect HUVECs from inflammatory and OS damage provoked by HSP serum via blocking TLR4/MyD88/NF-κB signals.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 98%

Protection of Proanthocyanidins Against HSP Serum-Induced Inflammation and Oxidative Stress on Human Umbilical Vein Endothelial Cells

Liu,

Wang,

Guo

et al. 2024

CCID

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Effect of dietary supplementation of macadamia oil on the growth, immune function, physio‐biochemical components and thyroid activity of growing rabbits

Jaber,

Nasr,

Al‐Sayed

et al. 2024

Animal Physiology Nutrition

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The current research aimed to assess the effects of dietary macadamia oil (MO) on carcass traits, growth performance, physio‐biochemical components, immune function, thyroid hormones and inflammation markers of growing rabbits. A total of 96 growing rabbits were randomly distributed into four treatments, with 24 rabbits in each group. The rabbits were fed a basal diet (control group) or a diet supplemented with MO at 0.5 (MO0.5), 1 (MO1.0) and 2 (MO2.0) mL/kg of diet for eight weeks. The daily body weight gain and feed conversion ratio showed a quadratic improvement with increasing levels of MO, and the optimal dose was 1.25 mL/kg of diet. Increasing levels of MO also had a quadratic effect on hepatic and renal functions. Dose‐response curves revealed that the optimal doses of MO were 1.50, 1.75 and 1.25 mL/kg of diet for total bilirubin, gamma‐glutamyl transferase, and creatinine respectively. A quadratic relationship was observed between the increased levels of MO and tumour necrosis factor‐α (p = 0.038), interleukin‐6 (p = 0.014) and immunoglobulins (p = 0.016 and IgM p = 0.026). Additionally, a linear relationship was observed between the increment in MO levels and both nitric oxide (p = 0.040) and interleukin‐4 (p = 0.001). The activities of superoxide dismutase and glutathione peroxidase showed a linear increase with increasing dietary MO content, while xanthine oxidase showed a linear decrease. Total antioxidant capacity showed quadratic improvement (p = 0.035) with the dietary treatment, with the optimal dose observed at 1.25 mL/kg of diet. The inclusion of MO in the diet had a linear effect on the activity of thyroxine (p = 0.001). Therefore, supplementation of MO at a dose of 1 or 1.5 mL/kg of diet in growing rabbits' diets can improve growth and carcass traits, sustain thyroid function by supporting immunity, and reduce oxidative/inflammation pathways.

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Effects of grape seed-derived proanthocyanidin B2 pretreatment on oxidative stress, endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells in renal ischemia–reperfusion injury model of mice

Cited by 2 publications

References 53 publications

Protection of Proanthocyanidins Against HSP Serum-Induced Inflammation and Oxidative Stress on Human Umbilical Vein Endothelial Cells

Protection of Proanthocyanidins Against HSP Serum-Induced Inflammation and Oxidative Stress on Human Umbilical Vein Endothelial Cells

Effect of dietary supplementation of macadamia oil on the growth, immune function, physio‐biochemical components and thyroid activity of growing rabbits

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