Effects of graphene oxide nanosheets in the polychaete Hediste diversicolor: Behavioural, physiological and biochemical responses

Pires, Adília; Figueira, Etelvina; Silva, M.S.S.; Sá, Carina; Marques, Paula A.A.P.

doi:10.1016/j.envpol.2022.118869

Cited by 18 publications

(7 citation statements)

References 78 publications

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“…Van Cauwenberghe et al 2015 [88] did not observe alterations in sugar, carbohydrate, and lipid content in the polychaete Arenicola marina exposed to polystyrene microspheres (10, 30, and 90 µm), and total energy content was not altered in H. diversicolor exposed to GO nanoflakes [89]. On the other hand, H. diversicolor exposed to PS NPs GO nanosheets for 28 days displayed decreased sugar content, which suggests that polychaetes use an immediately available energy source to fuel their defense mechanisms against GO nanosheet toxicity, since stored energy, as lipids and glycogen, was preserved [78].…”

Section: Discussionmentioning

confidence: 94%

“…For instance, a study evaluating the responses to waterborne polystyrene (PS) NPs of adult H. diversicolor, conducted by Silva et al (2020) [39], showed an increase in burrowing time with the increase in concentration (0.005, 0.05, 0.5 mg of PS NPS/L), apart from the highest two concentrations (5 and 50 mg of PS NPs/L), where the observed reduced burrowing activity, compared to the lowest concentrations, was associated with potential aggregation and lower availability of the particles. Other studies with copper and Graphene Oxide (GO) nanosheets also portrayed the loss of burrowing capacity of organisms [78,79]. AChE is an enzyme responsible, both in vertebrates and invertebrates, for the hydrolysis of the neurotransmitter acetylcholine in cholinergic synapses, which mainly regulates neuromuscular transmission.…”

Section: Discussionmentioning

confidence: 99%

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The Role of Life Stages in the Sensitivity of Hediste diversicolor to Nanoplastics: A Case Study with Poly(Methyl)Methacrylate (PMMA)

Neves,

Oliveira,

Frazão

et al. 2024

Toxics

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The presence of plastic particles in oceans has been recognized as a major environmental concern. The decrease in particle size increases their ability to directly interact with biota, with particles in the nanometer size range (nanoplastics—NPs) displaying a higher ability to penetrate biological membranes, which increases with the decrease in particle size. This study aimed to evaluate the role of life stages in the effects of poly(methyl)methacrylate (PMMA) NPs on the polychaete Hediste diversicolor, a key species in the marine food web and nutrient cycle. Thus, behavioral (burrowing activity in clean and spiked sediment) and biochemical endpoints (neurotransmission, energy reserves, antioxidant defenses, and oxidative damage) were assessed in juvenile and adult organisms after 10 days of exposure to spiked sediment (between 0.5 and 128 mg PMMA NPs/Kg sediment). Overall, the results show that H. diversicolor is sensitive to the presence of PMMA NPs. In juveniles, exposed organisms took longer to burrow in sediment, with significant differences from the controls being observed at all tested concentrations when the test was performed with clean sediment, whereas in PMMA NP-spiked sediment, effects were only found at the concentrations 8, 32, and 128 mg PMMA NPs/Kg sediment. Adults displayed lower sensitivity, with differences to controls being found, for both sediment types, at 8, 32, and 128 mg PMMA NPs/Kg sediment. In terms of Acetylcholinesterase, used as a marker of effects on neurotransmission, juveniles and adults displayed opposite trends, with exposed juveniles displaying increased activity (suggesting apoptosis), whereas in adults, overall decreased activity was found. Energy-related parameters revealed a generally similar pattern (increase in exposed organisms) and higher sensitivity in juveniles (significant effects even at the lower concentrations). NPs also demonstrated the ability to increase antioxidant defenses (higher in juveniles), with oxidative damage only being found in terms of protein carbonylation (all tested NPs conditions) in juveniles. Overall, the data reveal the potential of PMMA NPs to affect behavior and induce toxic effects in H. diversicolor, with greater effects in juveniles.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

The Role of Life Stages in the Sensitivity of Hediste diversicolor to Nanoplastics: A Case Study with Poly(Methyl)Methacrylate (PMMA)

Neves,

Oliveira,

Frazão

et al. 2024

Toxics

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“…After 24 h, graphene oxide (GO) at different concentrations (0, 0.1, 1.0, 10, and 20 mg/L) was added and the culture period extended for 96 h under the same growth conditions. The predicted environmental concentrations of this emerging contaminant in aquatic systems is projected to be approximately 0.001–1 mg/L [ 19 , 20 , 48 ]. Additionally, higher GO concentrations were used to ascertain the concentrations impacting the biofilm.…”

Section: Methodsmentioning

confidence: 99%

“…In the lab, 15 mL of biofilm were pipetted to plastic boxes paved with 15 ceramic tiles (4 × 5 cm), containing 500 mL of 10 times diluted Chu No.10 medium [47] and cultured under 10,100 lx in 12 h:12 h light/dark cycle at 18 ± 1 • C. After 24 h, graphene oxide (GO) at different concentrations (0, 0.1, 1.0, 10, and 20 mg/L) was added and the culture period extended for 96 h under the same growth conditions. The predicted environmental concentrations of this emerging contaminant in aquatic systems is projected to be approximately 0.001-1 mg/L [19,20,48]. Additionally, higher GO concentrations were used to ascertain the concentrations impacting the biofilm.…”

Section: Biological Materials and Experimental Setupmentioning

confidence: 99%

Effects of Graphene Oxide Nanosheets in Freshwater Biofilms

et al. 2023

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Graphene oxide (GO) properties make it a promising material for graphene-based applications in areas such as biomedicine, agriculture, and the environment. Thus, its production is expected to increase, reaching hundreds of tons every year. One GO final destination is freshwater bodies, possibly affecting the communities of these systems. To clarify the effect that GO may impose in freshwater communities, a fluvial biofilm scraped from submerged river stones was exposed to a range (0.1 to 20 mg/L) of GO concentrations during 96 h. With this approach, we hypothesized that GO can: (1) cause mechanical damage and morphological changes in cell biofilms; (2) interfere with the absorption of light by biofilms; (3) and generate oxidative stress, causing oxidative damage and inducing biochemical and physiological alterations. Our results showed that GO did not inflict mechanical damage. Instead, a positive effect is proposed, linked to the ability of GO to bind cations and increase the micronutrient availability to biofilms. High concentrations of GO increased photosynthetic pigment (chlorophyll a, b, and c, and carotenoids) content as a strategy to capture the available light more effectively as a response to the shading effect. A significant increase in the enzymatic (SOD and GSTs activity) and low molecular weight (lipids and carotenoids) antioxidant response was observed, that efficiently reduced oxidative stress effects, reducing the level of peroxidation, and preserving membrane integrity. Being complex entities, biofilms are more similar to environmental communities and may provide more accurate information to evaluate the impact of GO in aquatic systems.

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“…Furthermore, 21 days after the exposition, the time that the polychaetes needed to feed was recorded. For this assay, commercial fish food was added to the aquariums, and the time needed to polychaetes detect (the time that polychaetes need to come to the sediment surface) and grab the food (time that polychaetes needed to capture the food) in the sediment surface was recorded by video [ 58 ]. Nine organisms per condition were used for this endpoint (3 organisms per aquarium × 3 conditions).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and Behavioural Alterations Induced by Arsenic and Temperature in Hediste diversicolor of Different Growth Stages

Valente

Cardoso

Giménez

et al. 2022

IJERPH

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Contamination with Arsenic, a toxic metalloid, is increasing in the marine environment. Additionally, global warming can alter metalloids toxicity. Polychaetes are key species in marine environments. By mobilizing sediments, they play vital roles in nutrient and element (including contaminants) cycles. Most studies with marine invertebrates focus on the effects of metalloids on either adults or larvae. Here, we bring information on the effects of temperature increase and arsenic contamination on the polychaete Hediste diversicolor in different growth stages and water temperatures. Feeding activity and biochemical responses—cholinesterase activity, indicators of cell damage, antioxidant and biotransformation enzymes and metabolic capacity—were evaluated. Temperature rise combined with As imposed alterations on feeding activity and biochemical endpoints at different growth stages. Small organisms have their antioxidant enzymes increased, avoiding lipid damage. However, larger organisms are the most affected class due to the inhibition of superoxide dismutase, which results in protein damage. Oxidative damage was observed on smaller and larger organisms exposed to As and temperature of 21 °C, demonstrating higher sensibility to the combination of temperature rise and As. The observed alterations may have ecological consequences, affecting the cycle of nutrients, sediment oxygenation and the food chain that depends on the bioturbation of this polychaete.

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Effects of graphene oxide nanosheets in the polychaete Hediste diversicolor: Behavioural, physiological and biochemical responses

Cited by 18 publications

References 78 publications

The Role of Life Stages in the Sensitivity of Hediste diversicolor to Nanoplastics: A Case Study with Poly(Methyl)Methacrylate (PMMA)

The Role of Life Stages in the Sensitivity of Hediste diversicolor to Nanoplastics: A Case Study with Poly(Methyl)Methacrylate (PMMA)

Effects of Graphene Oxide Nanosheets in Freshwater Biofilms

Biochemical and Behavioural Alterations Induced by Arsenic and Temperature in Hediste diversicolor of Different Growth Stages

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