Effects of green light photobiomodulation on Dental Pulp Stem Cells: enhanced proliferation and improved wound healing by cytoskeleton reorganization and cell softening

Malthiéry, E; Chouaib, Batoul; Hernández-López, Ana María; Martin, Marta; Gergely, Csilla; Torres, J.; Cuisinier, Frédéric; Collart-Dutilleul, Pierre-Yves

doi:10.1007/s10103-020-03092-1

Cited by 13 publications

(4 citation statements)

References 43 publications

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“…The synergetic effect of biostimulation on regenerative endodontic therapy that was demonstrated in the present study was in agreement with the results of many in vitro studies (34,36,(39)(40)(41)(42) . It enhanced stem cell proliferative capacity of stem cells from human exfoliated deciduous teeth at power as low as 10 mW in two irradiation setting (41) .…”

Section: Discussionsupporting

confidence: 93%

“…Moreover, they have a rapid healing rate, large number of teeth that could be employed in the study as well as the availability of suitable cavity size that would facilitate the conduction of the study (18) . Low level laser therapy (LLLT) has a proven stimulatory effect on regenerative processes (34)(35)(36) without causing any significant temperature increase. It enhances cell proliferation, migration, differentiation as well as enhanced cellular metabolism and protein expression (37) .…”

Section: Discussionmentioning

confidence: 99%

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Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

et al. 2022

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Purpose: This study was carried out to evaluate the effect of biostimulation on the regenerative response of immature teeth with necrotic pulp and apical periodontitis in dogs. Materials and Methods: Seventy-two root canals were employed in the present study as follows: Sixty root canals for experimental groups and twelve as positive and negative controls. After the induction of pulp necrosis and apical periodontitis, roots were divided into two groups, group I: Regenerative Endodontic Therapy (RET) with subsequent biostimulation (seven sessions at 808 nm diode laser at output power of 300 mW for 90 seconds) and group II: RET without biostimulation. The groups were followed up for 1, 2 and 3 months (subgroups A, B&C). The maturation of the roots was assessed both radiographically and histologically. All data were statistically analyzed.Results: the application of biostimulation in group I demonstrated marked increase in root length, thickness and decrease in apical diameter compared to group II however, it was statistically significant only in subgroup C (12.43% versus 7.66%, 33.09% versus 17.81% and 38.12% versus 23.35 % at third month) respectively (p<0.05). Furthermore, it showed histologically the highest score of vital tissue infiltration and least inflammatory scores which was statistically significant. Conclusion: Biostimulation enhanced the response of immature teeth with necrotic pulp and apical periodontitis to regenerative endodontic therapy improving root maturation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

et al. 2022

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“…A recent study showed that DPSC treatment stimulated re-epithelialization and ameliorated collagen deposition in healing wounds; in dystrophic mice, DPSC treatment resulted in reduced fibrosis and collagen content, and infiltration of higher numbers of proangiogenic M2-like macrophages, which causes more satisfactory healing status (Martínez-Sarrà et al, 2017). Preclinical studies suggest that highintensity laser therapy and photobiomodulation therapy may collaborate with DMSCs to soften cells, reorganize cytoskeleton, and improve the efficacy of wound healing (Daigo et al, 2020;Malthiery et al, 2020).…”

Section: Wound Healingmentioning

confidence: 99%

Dental-Derived Mesenchymal Stem Cells: State of the Art

Ouchi

Cao

et al. 2021

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSCs) could be identified in mammalian teeth. Currently, dental-derived MSCs (DMSCs) has become a collective term for all the MSCs isolated from dental pulp, periodontal ligament, dental follicle, apical papilla, and even gingiva. These DMSCs possess similar multipotent potential as bone marrow-derived MSCs, including differentiation into cells that have the characteristics of odontoblasts, cementoblasts, osteoblasts, chondrocytes, myocytes, epithelial cells, neural cells, hepatocytes, and adipocytes. Besides, DMSCs also have powerful immunomodulatory functions, which enable them to orchestrate the surrounding immune microenvironment. These properties enable DMSCs to have a promising approach in injury repair, tissue regeneration, and treatment of various diseases. This review outlines the most recent advances in DMSCs’ functions and applications and enlightens how these advances are paving the path for DMSC-based therapies.

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“…LLLT, which activates tissues and cells at a power <500 mW to promote healing and reduce pain, is known as photobiomodulation therapy (PBMT) [ 4 , 5 ]. PBMT uses the beam of an LED light illuminator as well as a laser [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

Yarita,

Kitajima,

Morita

et al. 2024

Dentistry Journal

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This study aimed to determine the effect of photobiomodulation therapy induced by semiconductor laser irradiation on human dental pulp stem cell (hDPSC) proliferation and their differentiation into odontoblast-like cells (OLCs). The effects of various semiconductor laser irradiation conditions on hDPSCs were examined. Three groups were evaluated: a single laser irradiation at 6 h post-seeding, multiple laser irradiations up to four times every 4 days after the first dose, and a control with no laser irradiation. The cells were irradiated at 10, 30, and 150 mW using a semiconductor laser. The effect of laser irradiation on hDPSC differentiation into OLCs was also determined. Four groups were evaluated, including co-culture using basic medium and dentin discs, simple culture using OLC differentiation-inducing medium, co-culture using OLC differentiation-inducing medium and dentin discs, and control culture with basic medium. The expression of the nestin, ALP, DSPP, and DMP-1 genes was measured using real-time PCR. The multiple irradiation group irradiated at 30 mW exhibited significantly more cell proliferation than the control. The expression of nestin associated with differentiation into OLCs during each culture period tended to be lower, whereas DSPP and ALP expression was higher compared with that of the control. Multiple laser irradiations at a low power of 30 mW induced significant hDPSC proliferation and might induce differentiation into OLCs.

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Effects of green light photobiomodulation on Dental Pulp Stem Cells: enhanced proliferation and improved wound healing by cytoskeleton reorganization and cell softening

Cited by 13 publications

References 43 publications

Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

Dental-Derived Mesenchymal Stem Cells: State of the Art

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

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