Effects of green tea polyphenols and α-tocopherol on the quality of chilled cat spermatozoa and sperm IZUMO1 protein expression during long-term preservation

Wittayarat, Manita; Panyaboriban, Saritvich; Kupthammasan, Navapol; Otoi, Takeshige; Chatdarong, Kaywalee

doi:10.1016/j.anireprosci.2022.106926

Cited by 11 publications

(9 citation statements)

References 39 publications

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“…Another study showed that GTE contains catechins, epi catechins, gallocatechin, epigallocatechin, gallocatechin gallate, and Epigalocathecin gallate (EGCG). EGCG is the most promising bioactive compound due to its strong antioxidant activity [ 19 ], in turn improving sperm motility, and viability, and increase fertility [ 20 , 21 ]. EGCG is a kind of catechin that makes up 13% of the total polyphenols in GTE and can only be found naturally in GTE, while other types of catechins can only be found in fruits and vegetables [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

et al. 2023

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Background:The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, using an extender material in addition to semen increasing spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation.Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The divided samples into four treatments: T0 (no GTE added to the extender), T1 (0.05 mg GTE plus 100 mL extender), T2 (0.10 mg GTE plus 100 mL extender), and T3 (0.15 mg GTE plus 100 mL extender). The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and postthawing.Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion:Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.

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Section: Discussionmentioning

confidence: 99%

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

et al. 2023

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“…Some of them have the potential to act as scavengers, superoxidase, and hydroxyl and peroxy radicals released from oxidative phosphorylation. Together they can avoid the free radical's formation and protects the mitochondria, DNA, and plasma membrane of sperm [37][38][39]. Prevent oxidative damage could be achieved by a variety of diferent mechanisms, such as free radical scavenging, transition metal chelation and interactions with lipid membranes, proteins and nucleic acids [40].…”

Section: Discussionmentioning

confidence: 99%

The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk‐Based Extender Impacts Ram Sperm Quality

Allai

Druart

Terzioğlu

et al. 2023

Veterinary Medicine International

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Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH•) assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES) and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70% motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8% of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48, and 72 h. The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2% ETHEX in SM increased the sperm motility, viability, and membrane integrity and decreased the abnormality of spontaneous and catalyzed lipids peroxidation ( p < 0.05 ) up to 72 h. In addition, semen diluted with 1 and 2% ETHEX decreased the level of DNA fragmentation compared to the control group ( p < 0.05 ). In conclusion, the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on artificial insemination should be further studied.

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“…The IZUMO1 protein in sperm is a member of the immunoglobulin superfamily that contains an extracellular immunoglobulin domain [4] and is a crucial mediator in the interaction and fusion of sperm with eggs. This protein was recently reported in cat spermatozoa and is detected at 17 kDa by immunoblotting [5]. However, IZUMO1 alone might not be sufficient to facilitate sperm-egg fusion; instead, it may need assistance from other proteins such as those of the CRISP family [6].…”

Section: Introductionmentioning

confidence: 87%

“…The sperm of domestic cats (Felis catus) was collected from the cauda epididymides of six castrated males aged 1-4 years old, provided by the Poonnakan Veterinary Care Clinic, Songkhla province, Thailand, with three cats exhibiting good sperm quality (motility ≥ 80%) and three cats exhibiting low sperm quality (motility < 40%). The sperm collection method followed the procedures outlined by Wittayarat et al [5].…”

Section: Semen Collection and Evaluationmentioning

confidence: 99%

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Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii)

Wittayarat,

Kiatsomboon,

Kupthammasan

et al. 2024

Animals

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Effective wild cat conservation programs with assisted reproductive technologies are being developed in different parts of the world. The flat-headed cat, fishing cat, and Asiatic golden cat are three species among nine wild Felidae in Thailand that are in need of urgent conservation efforts. Here, we assessed routine sperm characteristics and we report the detection of protein biomarkers related to the fertilization process, IZUMO1 and the CRISP family, and apoptotic markers, active or cleaved caspase-3, in semen samples collected from these wild cats. IZUMO1 was located in the equatorial segment of the sperm head, which is the region involved in gamete interaction. The highest levels of IZUMO1 were found in both the sperm pellet and the seminal plasma of the flat-headed cat, as determined by immunoblotting. CRISP2, a sperm–egg fusion assisting protein, and CRISP3 were found in both the sperm pellet and the seminal plasma, and the highest levels were observed in the fishing cat. Positive correlations between certain semen parameters and IZUMO1, CRISP2, and CRISP3 expression were also demonstrated. Cleaved caspase-3 was found in all sperm samples in all three species and was associated with an increase in DNA fragmentation and a decrease in certain semen characteristics such as motility, viability, and intact acrosomes. Our results suggest that the analysis of IZUMO1, the CRISP family, and cleaved caspase-3, along with the routine sperm characteristics, may allow for better success in breeding management in wild Felidae, particularly in the flat-headed cat and the fishing cat.

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Effects of green tea polyphenols and α-tocopherol on the quality of chilled cat spermatozoa and sperm IZUMO1 protein expression during long-term preservation

Cited by 11 publications

References 39 publications

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk‐Based Extender Impacts Ram Sperm Quality

Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii)

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