Effects of Grf(1–40) and Domperidone on Gh Secretion in Normal Man

Giusti, Massimo; Mazzocchi, G.; Sessarego, P; Mignone, D.; Lomeo, A.; Monachesi, Mario; Giordano, Giulio

doi:10.1111/j.1365-2265.1984.tb03220.x

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…This is compatible with recent data demonstrating that metoclopramide also has no effect on the GH response to GHRH [17]. We were unable to replicate the findings of Giusti et al [13] that domperidone enhances the GH response to GHRH, al though the total dose and dosage regimens were slightly different in the two studies. If dopamine receptors do in deed stimulate GH release via inhibition of somatostatin, these results imply that under basal conditions hypotha lamic somatostatin is under little or no dopaminergic con trol.…”

Section: Discussioncontrasting

confidence: 39%

Dopaminergic and Cholinergic Influences on the Growth Hormone Response to Growth Hormone-Releasing Hormone in Man

et al. 1987

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It is well established that compounds that modify dopaminergic and cholinergic activity in man may induce changes in circulating growth hormone (GH). We have, therefore, investigated the effect of a dopamine agonist, bromocriptine, and a dopamine antagonist, domperidone, as well as a muscarinic cholinergic antagonist, pirenzepine, on the GH response to an analogue of GH-releasing hormone (GHRH) in normal male subjects. GHRH(1–29)NH₂ induced a rise in serum GH that was augmented by bromocriptine, antagonized by pirenzepine, but was unaltered by domperidone. As this dose of GHRH(l-29) NH₂ has been shown to be maximally stimulatory to GH release, it is suggested that there are dopamine stimulatory and cholinergic inhibitory receptors to GH release independent of GHRH in man.

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Section: Discussioncontrasting

confidence: 39%

Dopaminergic and Cholinergic Influences on the Growth Hormone Response to Growth Hormone-Releasing Hormone in Man

et al. 1987

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“…The peptide was diluted and stored as previously reported (Giusti et al 1984) and the test was performed by iv administration of 0.01 µg/kg body weight of GRH (hp-GRF 1-40, CRB Cambridge) in 8 CAPD, 8 CT, 9 NC and 10 NA subjects. In order to save blood the number of samples was reduced: the venipuncture was carried out at 09.00 h. The following hormones were measured: GH, LH, FSH, TSH, Prl, and F. The insulin test (0.1 U/kg body weight, iv) was carried out in 5 CAPD, 5 CT, 10 NC and 9 NA subjects on blood samples taken every 30 min for 2 h measuring GH and glycaemia.…”

Section: Methodsmentioning

confidence: 99%

Growth hormone response to growth hormone-releasing hormone in normal and uraemic children. Comparison with hypoglycaemia following insulin administration

Bessarione¹,

Perfumo²,

Giusti³

et al. 1987

Acta Endocrinologica

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The uraemic syndrome is characterized by several endocrinological disturbances. This study was undertaken in order to evaluate the GH response to growth hormone-releasing hormone (GRH) in children with chronic renal failure (CRF) and to compare the results with those observed after insulin hypoglycaemia. Twenty-two children with CRF, 10 undergoing continuous ambulatory peritoneal dialysis (CAPD) and 12 on conservative treatment (CT), age ranges 2\p=n-\15 years, were studied and the data were compared with those from 14 children with normal renal function and normal hormonal behaviour, affected by short stature (NC), and those form 13 healthy adult volunteers (NA). The GRH test (1 \g=m\g/kgbody weight, iv) was carried out in 8 CAPD, 8 CT, 9 NC and 10 NA subjects. The blood samples were taken every 30 min for 3 h in CAPD and CT and for 2 h in NC and NA starting at 09.00 h. The following hormones were measured: GH, LH, FSH, Prl, TSH and cortisol (F). The insulin test (0.1 U/kg body weight, iv) was carried out in 5 CAPD, 5 CT, 10 NC and 9 NA on blood samples taken every 30 min for 2 h, measuring GH and glycaemia. No adverse effects were observed after the infusion of GRH. GRH administration induced a prompt response in all subjects, but GH plasma levels were significantly higher in uraemic children than in adults (peak value of 43.5 \ m=+-\ 8.2, 45.0 \ m=+-\ 8.4, 27.8 \ m=+-\ 6.0; 13.5 \ m=+-\ 2.6 \g=m\g/ml in CAPD, CT, NC and NA, respectively). The secretory areas were significantly narrower in NC (P < 0.05) and NA (P < 0.01) than in CAPD, and in NA than in CT (P < 0.01). The GH response to insulin did not differ in the 4 groups. The secretory area in CAPD and CT was wider after GRH than after insulin. The GH peak value of CAPD and NC was significantly higher after GRH than following insulin. No significant variation in TSH, LH and FSH was observed after the infusion of the neuropeptide, whereas Prl and F showed a reduction.The behaviour of Prl and F in NA and NC was similar after placebo and GRH.Our data show: a) There is a greater response of GH to GRH in children than in adults; b) compared with the insulin test, GRH stimulation seems to be a reliable means of evaluating GH secretion both in normal and uraemic children owing to the absence of qualitatively different responses and to the freedom from the adverse effects or risks which follow insulin infusion; c) GRH administration does not induce any significant variation on other hypophyseal hormones and the reduction of Prl and F seems to follow the normal sl eep\ x=req-\ wake and circadian behaviour.

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Effect of simultaneous administration of GHRH (1–40) and TRH on GH, PRL and TSH secretion in normal man

Giusti

Lomeo

Mazzocchi

et al. 1986

J Endocrinol Invest

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The GHRH test represents a new tool in the study of secretion in man. Nine normal fasting males received on separate occasions in random order 1) GHRH 1-40 (1 microgram/Kg bw) iv at time 0; 2) TRH (6 micrograms/min) infusion between -30 and +120 min; 3) GHRH 1-40 (1 microgram/Kg bw) iv at time 0 plus TRH (6 micrograms/min) infusion between -30 and +120 min. Blood samples were drawn for GH, PRL and TSH at -90, -60, -30, 0 min and then every 15 min for 2 h. GHRH significantly increased GH in all subjects. The same GH response was found during GHRH plus TRH test. No effect was found either on PRL and TSH secretion after GHRH administration, or on GH pattern after TRH administration. A significant decrease of TSH, but not of PRL response was observed after GHRH plus TRH administration in comparison to TRH alone. These results underline that the inhibitory effect exerted by TRH on GH secretion during some experimental conditions is not linked to a pituitary interference between GHRH and TRH. The difference in TSH secretion, following GHRH plus TRH in comparison with TRH alone, could be due to a GHRH-induced central inhibitory mechanism, probably GHRH-related.

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Effects of Grf(1–40) and Domperidone on Gh Secretion in Normal Man

Cited by 10 publications

References 19 publications

Dopaminergic and Cholinergic Influences on the Growth Hormone Response to Growth Hormone-Releasing Hormone in Man

Dopaminergic and Cholinergic Influences on the Growth Hormone Response to Growth Hormone-Releasing Hormone in Man

Growth hormone response to growth hormone-releasing hormone in normal and uraemic children. Comparison with hypoglycaemia following insulin administration

Effect of simultaneous administration of GHRH (1–40) and TRH on GH, PRL and TSH secretion in normal man

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