“…The use of sorghum in ethanol production has increased in recent years; 30% to 35% of the total crop was used for ethanol production in 2011 (USDA, 2011). Previous studies have shown that the amylose-to-amylopectin ratio, starch content, protein digestibility, protein content, free amino nitrogen, formation of amylose-lipid complex, and particle size of ground grain meal have significant effects on ethanol yield and fermentation efficiency (Wu, 1989;Zhan et al, 2003;Wu et al, 2006Wu et al, , 2007Wu et al, , 2008Pérez-Carrillo and Serna-Saldívar, 2007;Yan et al, 2009Yan et al, , 2010Yan et al, , 2011. Therefore, sorghum grain qualities are important and greatly affect end-use product qualities.…”