Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

Lebedev, V. T.; Arkaev, Mikhail; Dremova, Mariya; Pozdniakov, Ivan; Shestibratov, Konstantin A.

doi:10.3390/plants8010003

Cited by 22 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result could be explained by the rising proportion of plantlets showing hyperhydricity symptoms during the eight-week exposure to Al, which reached about 30% in the presence of a concentration of 1 mM AlCl 3 . Usually, the hyperhydricity of in vitro-cultured plantlets is due to several factors such as the composition of culture medium, notably the concentration of phytohormones [39] and the type of gelling agent [40]. Van den Dries et al [41] proposed that hyperhydricity is caused by a water logging of the apoplast, leading to an impairment of gas exchanges.…”

Section: Discussionmentioning

confidence: 99%

Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets

Bonet

Lelu‐Walter²,

Faugeron

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

Phytoextraction of Cd is a growing biotechnology although we currently know few Cd hyperaccumulators, i.e., plant species able to accumulate at least 0.1 mg Cd g(-1) dry weight in aerial organs. Owing their deep root system and high biomass, trees are more and more preferred to herbaceous species for phytoextraction. Assuming that conifers could be relevant models under cold climates, we investigated cadmium tolerance of the hybrid larch Larix × eurolepis Henry (Larix decidua × Larix kaempferi) and the efficiency of this species to store this metal. In vitro grown larches were chosen in order to reduce time of exposure and to more rapidly evaluate their potential efficiency to accumulate Cd. One-month-old plantlets were exposed for 2 and 4 weeks to 250 and 500 μM Cd. Results showed that they tolerated a 4-week exposure to 250 μM Cd, whereas the content of photosynthetic pigment strongly dropped in plantlets growing in the presence of 500 μM Cd. In the presence of 250 μM Cd, shoot growth slightly decreased but photosynthetic pigment and total soluble carbohydrate contents were not modified and no lipid peroxidation was detected. In addition, these plantlets accumulated proline, particularly in shoots (two to three times more than control). In roots, Cd concentration in the intracellular fraction was always higher than in the cell wall fraction contrary to shoots where Cd concentration in the cell wall fraction increased with time and Cd concentration in the medium. In shoots, Cd concentration was lower than in roots with a ratio of 0.2 after 4 weeks of exposure but stayed around 0.2 mg g(-1) dry weight, thus a value higher than the threshold requested for Cd hyperaccumulators. Hybrid larch would thus be a relevant candidate for field test of Cd phytoextraction.

show abstract

Section: Discussionmentioning

confidence: 99%

Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets

Bonet

Lelu‐Walter²,

Faugeron

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…Most importantly, the plantlets developed through the ex vitro rooting method, similar to the natural root system of S. grosvenorii, had lateral roots without any callus at the base of microcuttings, making the ex vitro rooting method more suitable than the in vitro development of roots. Lebedev et al (2019) reported that ex vitro rooting of raspberry could reduce the cost and labor intensity of plant micropropagation. Unrooted microshoots of the primocane-fruiting raspberry cultivar ' Atlant' were simultaneously rooted and acclimatized in the greenhouse with a survival rate of up to 97.2%.…”

Section: Discussionmentioning

confidence: 99%

“…Ex vitro rooting is not only advantageous for the acclimatization of plantlets, but also for the reduction of labor and costs of micropropagation (Annapurna & Rathore, 2010;Benmahioul et al, 2012;Huang et al, 2011;Phulwaria, Rai, Harish, et al, 2012;Phulwaria et al, 2011;. Successful ex vitro rooting has been applied in the micropropagation of various plant species, including blueberries (Vaccinium corymbosum L. and Vaccinium angustifolium × corymbosum) (Isutsa & Pritts, 1994), raspberries (Rubus idaeus L.) (Lebedev et al, 2019), pistacia (Pistacia vera L.) (Benmahioul et al, 2012), and other ornamental and medicinal species (Annapurna & Rathore, 2010;Huang et al, 2011;Phulwaria, Rai, Harish, et al, 2012;Phulwaria et al, 2011;Podwyszynska & Gabryszewska, 2003;Yan et al, 2010). Reports on the simultaneous rooting and acclimatization of Pyrus species on different substrates are scarce and show little success.…”

Section: Introductionmentioning

confidence: 99%

An Optimized Micropropagation Protocol by Ex Vitro Rooting of Pear Rootstock OHF 333 (Pyrus communis L.)

Dimitrova

Nacheva

2021

Acta Agrobot

View full text Add to dashboard Cite

Pear rootstock OHF 333 ( Pyrus communis L.), which is included in the US ‘Old Home’ × ‘Farmingdale’ series, is characterized by good compatibility with most other varieties, high yields, and a moderate degree of resistance to fire blight. Micropropagation in vitro has shown promise for rapid, large-scale cloning of disease-free plant material throughout the year. However, pear shoots are often recalcitrant to rooting, and this process is highly genotype-dependent. This study aimed to improve the micropropagation protocol by ex vitro rooting of pear rootstock OHF 333 ( Pyrus communis L. ‘Old Home’ × ‘Farmingdale’). Charkor, a new plant growth regulator of natural origin was used, which contains metabolism products of symbiotic fungus-endophytes of ginseng roots, as an alternative to synthetically produced plant growth regulators (PGRs). Microcuttings were obtained from in vitro cultured shoots and subjected to four different treatments for ex vitro rooting: 1 g L −1 1-naphthaleneacetic acid (NAA) (as a powder), 0.5 mL L −1 Charkor for 3 hr or 6 hr, or the same concentration of Charkor prepared as a powder. Microshoots dipped in sterile distilled water with no additional hormonal treatments served as controls. Cultures were kept in a growth chamber under a 16-hr photoperiod, with air humidity maintained close to 100% (above 96%) for 2 weeks and then gradually reduced to 60%. Data on final acclimatization rate (survival rate; %), mean number of roots per plant, stem and root length, mean number of leaves per plant, and final acclimatization rate were collected 90 days after transplanting to ex vitro conditions. All treatments induced a successful acclimatization rate of more than 31%. The highest survival rate (86%) and longest stems were achieved by treatment with 0.5 mL L −1 Charkor for 6 hr. The greatest mean number of roots per plant, root length, and number of leaves was achieved in the variant treated with 1 g L −1 powdered NAA.

show abstract

“…In this experiment, 2iP and TDZ caused the vitrification of axillary shoots. Therefore, it is important to select a proper concentration of cytokinin, because high concentrations cause vitrification and somaclonal variations [27]. Cytokinins (BA, 2iP, and TDZ) had multifunction in the physiological processes and the development of the plant as cell division and enlargement and stimulation of protein synthesis and enzyme activity [28].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

Ghareb

Ibrahim

Hegazi

2020

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background: Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. Results: To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA 3) , 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of multiple axillary shoots were rooted on quarter-strength MS medium supplemented with 4.92 μM indole-3-butyric acid (IBA) and 10.75 μM NAA. Rooted plants were transferred to pots containing a soil-peat mixture (1: 2 v/v) and successfully acclimatized in the greenhouse. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of Silene schimperiana was carried out using two chloroplast DNA markers (cpDNA): 1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) and RNA polymerase subunit (rpoC1) and a nuclear ribosome DNA marker (ncDNA), internal transcribed spacer (ITS). Phylogenetic analysis revealed a successful identification of Silene schimperiana on the species and genus levels and supported the inclusion of Silene schimperiana in genus Silene. Conclusions: In this study, a relevant in vitro propagation method was established to facilitate the recovery of Silene schimperiana, in addition to DNA barcoding of the plant as a tool for effective management and conservation of plant genetic resources.

show abstract

Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

Cited by 22 publications

References 40 publications

Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets

Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets

An Optimized Micropropagation Protocol by Ex Vitro Rooting of Pear Rootstock OHF 333 (Pyrus communis L.)

In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

Contact Info

Product

Resources

About