Effects of Growth Regulators, Media and Explant Types on Microtuberization of Potato

Yağız, Ayten Kübra; Yavuz, Caner; Tarım, Cehibe; Demirel, Ufuk; Çalışkan, Mehmet Emin

doi:10.1007/s12230-020-09801-4

Cited by 17 publications

(9 citation statements)

References 35 publications

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“…Drought‐sensitive Agria and drought‐tolerant Sante cultivars were used in the present study (Alhoshan & Ramin, 2019 ; Demirel et al, 2020 ). The potato plantlets were initially propagated with nodal culture in standard Murashige and Skoog (MS) medium supplemented with 3% sucrose and 8% phytoagar (Yagiz et al, 2020 ). Plants were grown under white fluorescence light (16/8 h light/dark photoperiod) and 25/16°C day/night temperatures in a growth room before stress treatment.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Aksoy,

Yavuz,

Yagiz

et al. 2024

Plant Direct

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GATA is one of the prominent transcription factor families conserved among many organisms in eukaryotes and has different biological roles in many pathways, particularly in light regulation in plants. Although GATA transcription factors (TFs) have been identified in different crop species, their roles in abiotic stress tolerance have not been studied in potato. In this study, we identified 32 GATA TFs in potato (Solanum tuberosum) by in silico analyses, and expression levels of selected six genes were investigated in drought‐tolerant (Sante) and sensitive (Agria) cultivars under light, drought, and combined (light + drought) stress conditions. According to the phylogenetic results, StGATA TFs were divided into four main groups (I, II, III, and IV) and different sub‐groups in I and II (eight and five, respectively). StGATA genes were uniformly localized to each chromosome with a conserved exon/intron structure. The presence of cis‐elements within the StGATA family further supported the possible involvement in abiotic stress tolerance and light response, tissue‐specific expression, and hormonal regulation. Additional PPI investigations showed that these networks, especially for Groups I, II, and IV, play a significant role in response to light and drought stress. Six StGATAs were chosen from these groups for expressional profiling, and their expression in both Sante and Agria was mainly downregulated under purple and red lights, drought, and combined stress (blue + drought and purple + drought). The interactomes of selected StGATAs, StGATA3, StGATA24, and StGATA29 were analyzed, and the accessions with GATA motifs were checked for expression. The results showed that the target proteins, cyclin‐P3‐1, SPX domain‐containing protein 1, mitochondrial calcium uniporter protein 2, mitogen‐activated protein kinase kinase kinase YODA, and splicing factor 3 B subunit 4‐like, mainly play a role in phytochrome‐mediated stomatal patterning, development, and activity. Understanding the interactions between drought stress and the light response mechanisms in potato plants is essential. It will eventually be possible to enhance potato resilience to climate change by manipulating the TFs that play a role in these pathways.

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Section: Methodsmentioning

confidence: 99%

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Aksoy,

Yavuz,

Yagiz

et al. 2024

Plant Direct

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“…So far, a few studies were used TDZ either alone or in combination with other growth regulators for microtuberization in potato (Kefi et al 2000;Lajayer et al 2011;Kianmehr et al 2012;Kepenek et al 2017;Yagiz et al 2020), and only one report was found on the effect of TDZ and BAP on microtuberization in potato (Abd Elaleem et al 2015).…”

Section: The Effect Of Bap and Tdz On Microtuberization In Potato CV ...mentioning

confidence: 99%

Effect of Thidiazuron and 6-Benzylaminopurine on in vitro microtuberization in potato cv. Spunta.

Ibrahim¹

2022

Annals of Agricultural Science, Moshtohor

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The present study aimed to investigate the effect of TDZ and BAP on in vitro microtuberization in potato cv. Spunta. Different concentrations (4, 6, 8, and 10 mg/l) of BAP either alone or in combination with three concentrations (1, 2, and 3 mg/l) of TDZ were used for investigating their influence on microtuberization. The results showed that the individual treatment of BAP at the high level (10 mg/l) recorded the highest value of the average number of the produced microtubers/jar (7.1 microtubers/jar) compared to the other treatments (4, 6, and 8 mg/l). The combinational treatments of BAP and TDZ exhibited an increase compared to the individual corresponding concentration of BAP and this increase reached a significant level with the concentrations (4, 6, and 8 mg/l) of BAP, while a slight increase was recorded with 10 mg/l. Furthermore, the combinational treatment of BAP (6 mg/l) and TDZ (1 mg/l) resulted in the highest average number of the produced microtubers/jar (8.1 microtubers/jar) which represents about 41.1 % increase over the individual treatment of BAP (6 mg/l) with the highest average weight of microtuber (116.94 mg) and the highest average diameter of microtuber (5.3 mm). These results confirm the positive effect of the combinational treatment of BAP and TDZ on enhancing microtuber formation in potato cv. Spunta. Therefore, we report here that an efficient protocol for microtuberization in potato cv. Spunta was established. In addition, these promising results are important for developing advanced technology for microtubers mass production in Egypt.

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“…One avenue of investigation has been the use of micropropagation techniques. This, when combined with methods for disease detection, has allowed for the production of seedlings with signi cantly improved phytosanitary status with e cient multiplication rates (Yagiz et al 2020). Moreover, a range of bioreactors have been developed (Valdiani et al 2019) with various applications.…”

Section: Main Textmentioning

confidence: 99%

Agronomic Performance of Temporary Immersion Bioreactor – Derived Potato Microtubers in a Peruvian Low Input Cropping Agriculture System

Figueroa¹,

Tapia²,

Hajari³

et al. 2021

Preprint

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Potato cultivation is limited by a lack of access to quality propagation material. The application of micropropagation techniques combined with the diagnosis and sanitation of the main pathogens of the crop, has contributed to increased production efficiencies. In this regard, the use of temporary immersion bioreactors (TIBs) has improved the quality of microtubers micropropagated along with savings in costs of production. With the final goal of applying these technologies for commercial production, the current study investigated the agronomic performance of Peruvian Canchan potato microtubers derived from TIBs (basic agamic seed 1 and 2) under low-input agro-technology in the coastal zone of Peru. The results indicated that following 75 d of growth, plants derived from microtubers produced in TIBs displayed slower vegetative growth than those from conventional tubers. However, at harvest, these differences were no longer apparent. Although plants raised from conventional tubers produced the highest fresh mass of tubers, significantly more propagules were produced by plants regenerated from basic agamic seed 1 and 2 derived from micropropagation in liquid media. These results demonstrate that much more planting material (seed tubers) can be obtained from microtubers in the field (basic agamic seed 1) than from the conventional commercial seed tubers.

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Effects of Growth Regulators, Media and Explant Types on Microtuberization of Potato

Cited by 17 publications

References 35 publications

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Effect of Thidiazuron and 6-Benzylaminopurine on in vitro microtuberization in potato cv. Spunta.

Agronomic Performance of Temporary Immersion Bioreactor – Derived Potato Microtubers in a Peruvian Low Input Cropping Agriculture System

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