Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (&amp;lt;i&amp;gt;Mentha pi-perita&amp;lt;/i&amp;gt;) Micropropagated &amp;lt;i&amp;gt;in Vitro&amp;lt;/i&amp;gt;

Santoro, Maricel Valeria; Nievas, Fiorela; Zygadlo, Julio Alberto; Giordano, Walter; Banchio, Erika

doi:10.4236/ajps.2013.45a008

Cited by 54 publications

(28 citation statements)

References 23 publications

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“…The use of cytokinin in the cultivation in vitro of peppermint (Mentha piperita) increased the number of nodes, branches and leaves (although a reduced shoot fresh matter) and improved the performance of essential oil extraction, per fresh mass unit. It was also observed that these plants had much longer roots and with higher dry weight than those of control plants (Santoro et al, 2013).…”

Section: Resultsmentioning

confidence: 82%

Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.)

2014

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The production of medicinal plants as raw material for industry must associate quality with biomass formation and, with this purpose, the application of plant growth regulators has been studied in these crops. The objective of this study was to evaluate the effect of a biostimulant on growth, inflorescence production and flavonoid content in marigold. The experiment was conducted in a greenhouse and the treatments consisted of increasing doses of the biostimulant (0, 3, 6, 9, 12 and 15 mL L-1) applied by foliar spraying in ten consecutive applications. The experiment was arranged in a completely randomized design, with six treatments and ten repetitions. The number of leaves and flowerheads and dry matter of roots increased linearly with increasing doses of the growth promoter, with 20%, 36.97% and 97.28% increases, respectively, compared with the control. The total dry mass and shoot dry mass showed maximum values at the highest dose tested of 15 mL L-1 (with increases of 40.09% and 46.30%, respectively). Plant height and flavonoid content reached the highest values at a dose of 6 mL L-1. The biostimulant promoted the development of marigold and positively influenced the synthesis of the secondary compound of medicinal interest. Among the tested doses, the application of rates between 6 and 9 mL L-1 of the biostimulant is recommended for more efficient large-scale production of marigold.

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Section: Resultsmentioning

confidence: 82%

Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.)

2014

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“…(2007) over Salvia officinalis, S. albicaulis and S. dolomitica plants on different stages of aseptic culture demonstrated the presence of shield-shaped glandular scales that produced high quality essential oils. Glandular trichomes were also described on leaf surface in Mentha piperita regenerants (Santoro et al, 2013). Glandular hairs are formed from epidermal outgrowths that divide asymmetrically to produce basal cells (Turner et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Brailko¹,

Митрофанова²,

Лесникова-Седошенко³

et al. 2017

AgricultForest

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SUMMARYLavender and lavandin are widely used in food, pharmaceutical, cosmetic industries and ornamental gardening. Gene pool collection of Lavandula angustifolia and lavandin is presented in Nikita Botanical Gardens plots and the same collection is being created in vitro.Initial explants of L. angustifolia, its cultivars 'Belyanka', 'Record' and L. hybrida -'Rabat' and 'Snezhnyi Bars' were introduced in vitro. Plant regeneration occurred on MS medium supplemented with 0.3-0.5 mg l -1 kinetin, 0.025 mg l -1 NAA, 0.25 mg l -1 GA 3 . Samples were analyzed after 2 and 8 months of culture.Structural analysis of regenerated plants vegetative organs enabled assessment of their morphogenetic capacity. Under in vitro culture 2-5 microshoots/explants were produced with the height of 2.3 -8.2 cm. Leaves were opposite, sessile linear or linear-lanceolate, with entire edges curved outward, densely pubescent, and 10-30 leaves/microshoot. Leaf lamina length was 0.9-1.5 cm. Leaves were bifacial, amphystomatic, lamina thickness -132-215 µm, palisade rate was 0.38-0.41 after 2 months culture and 0.56-0.58 -after 8 months. Epidermal thickness was 8-19 µm, cells were relatively small with thin, cellulose walls covered with cuticle. Stomata are of anomocyte type, small, raised above the epiderm cells, 48-150 pcs/mm 2 on adaxial surface, 115-432 pcs/mm 2 -on abaxial one. Essential oils are produced in simple and glandular, unicellular and multicellular hairs of epidermal origin, located above the leaf chlorophyll tissue. Features of xeromorphic structure appeared at a long-term in vitro cultivation.

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“…Micropropagation is appropriate for species that cannot be efficiently propagated by conventional horticultural techniques and an important tool to select, multiply, and conserve the critical genotypes of medicinal plants in particular (Debnath et al, 2006;Tripathi and Tripathi, 2003). Various medicinal plants and plants of Lamiaceae family in particular have been cloned through micropropagation protocols (Aicha et al, 2013;Chaturvedi et al, 2007;Debnath et al, 2006;Gonc xalves and Romano, 2013;Santoro et al, 2013;Shtereva et al, 2015;Zuzarte et al, 2010). Moreover, germination of seeds in some species, which show low or no germination using conventional techniques, due to dormancy or specific germination requirements, could be greatly increased using in vitro methods (Fay, 1994).…”

Section: )mentioning

confidence: 99%

“…Moreover, BA promoted shoot multiplication in several Lavandula sp. (Gonc xalves and Romano, 2013;Zuzarte et al, 2010), while 6-benzyl-aminopurine (BAP) was used in micropropagation of Mentha piperita (Santoro et al, 2013). In T. fruticans, BAP and kinetin induced similar shoot proliferation (Frabetti et al, 2009); while in T. polium, kinetin gave higher shoot production than BA (Al-Qudah et al, 2011).…”

Section: Pretreatmentmentioning

confidence: 99%

In Vitro Seed and Clonal Propagation of the Mediterranean Aromatic and Medicinal Plant Teucrium capitatum

Papafotiou¹,

Martini²

2016

horts

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The effect of various pretreatments, culture conditions, and storage time on in vitro germination of seeds, as well as the effect of explant origin and plant growth regulators on in vitro propagation of Teucrium capitatum L. (Teucrium polium sp. capitatum Arcang., Lamiaceae) were examined. Seeds, collected from native plants and stored at room temperature for 3, 7, and 12 months, were cultured for germination in vitro in petri dishes with solid half-strength (½) Murashige and Skoog (1962) growth medium (MS) at 5, 10, 15, 20, 25, and 30 °C, and 16 hours light or continuous darkness. Pretreatments, such as cold stratification, scarification with sandpaper, dipping in concentrated sulfuric acid (H2SO4 > 95%), or dipping in boiling water were tested. Seeds without any pretreatment germinated at lower than 10%. Dipping in concentrated H2SO4 for 15 or 20 minutes was the most effective pretreatment, but still seed germination achieved was low (36%). Seeds preserved their germination capacity for at least 1 year, and germinated satisfactorily at a wide temperature range, from 15 to 25 °C (optimum), while photoperiod did not affect seed germination. Explants excised from in vitro-grown seedlings were established in vitro on MS medium with 1.0 mg·L−1 6-benzyladenine (BA) at much higher rates (≈90%) compared with those collected from plants grown from cuttings in a greenhouse (25%), while explants collected from adult wild plants failed to do so. Explants from seedlings showed strong variability in their response; those excised from branched seedlings formed shoots at significantly higher percentage (90%) at the establishment stage (cultured on MS medium with 0.5–2.0 mg·L−1 BA) than explants excised from unbranched seedlings (36% to 43%), while during subcultures on MS medium with 1.0 mg·L−1 BA, explants from branched seedlings also showed higher multiplication rates than those from unbranched ones. BA at 0.5–2.0 mg·L−1 induced shoot multiplication during both establishment and multiplication stages (7–8 and 14–15 shoots per explant, respectively), while kinetin and 6-γ-γ-(dimethylallylamino)-purine (2iP) were less effective than BA, and zeatin the least appropriate. Microshoot rooting was enhanced by 1-week culture on (½) MS medium with 1.0–4.0 mg·L−1 indole-3-butyric acid (IBA), followed by transfer to auxin-free, (½) MS medium (93% to 98%, 7–8 roots per microshoot), compared with culture on the same medium continuously for 5 weeks (69% to 80%, 5–6 roots per microshoot) or at lower IBA concentrations. Plantlets were acclimatized to ex vitro conditions at 98% on a peat–perlite (1:1, v/v) mixture.

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Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (<i>Mentha pi-perita</i>) Micropropagated <i>in Vitro</i>

Cited by 54 publications

References 23 publications

Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.)

Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.)

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

In Vitro Seed and Clonal Propagation of the Mediterranean Aromatic and Medicinal Plant Teucrium capitatum

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