Effects of growth stage and hardening conditions on the association between frost resistance and the expression of the cold-induced protein COR14b in barley

Regulatory genes involved in the determination of frost tolerance in temperate cereals

“…differences in threshold induction temperatures of these genes were precisely mapped to the same 0.7 cM region including the differentially regulated CBF genes. Higher threshold induction temperatures of COR14b in frost resistant genotypes relative to frost susceptible ones have been also reported for common wheat [68] and barley [70,71]. The differences in COR14b induction between frost susceptible and frost tolerant genotypes observed at mild cold temperatures disappear when plants are exposed to lower temperatures (<4 8C) [41,68].…”

The development of frost tolerance and DHN5 protein accumulation in barley (Hordeum vulgare) doubled haploid lines derived from Atlas 68×Igri cross during cold acclimation

“…These discrepancies can be explained by the known fact that the acquired FT is a complex trait and the level of dehydrin (or COR/LEA protein) accumulation represents only one component of plant FT level, i.e., the resulting FT is determined not only by COR/LEA accumulation, but also by other factors -accumulation of saccharides, low-molecular compatible solutes, proline content, etc. The fact that the level of COR/LEA accumulation does not necessarily correlate with the acquired FT has recently been reported by Crosatti et al (2008) on chloroplast-located LEA III protein COR14b in five barley cultivars subjected to CA treatment. Crosatti et al (2008) monitored plant FT and COR14b accumulation under field conditions during winter and reported the difference between COR14b and plant FT in the samples from later sampling dates.…”

“…The fact that the level of COR/LEA accumulation does not necessarily correlate with the acquired FT has recently been reported by Crosatti et al (2008) on chloroplast-located LEA III protein COR14b in five barley cultivars subjected to CA treatment. Crosatti et al (2008) monitored plant FT and COR14b accumulation under field conditions during winter and reported the difference between COR14b and plant FT in the samples from later sampling dates. Our results, which were gained in controlled conditions (growth chambers), support the findings of Crosatti et al, as we also observed the discrepancy between DHN5 accumulation and FT in later stages of CA treatment.…”

Low temperature tolerance in plants: Changes at the protein level

“…In particular, in the case of COR14b, a chloroplast-located soluble protein of unknown function, it seems that a molecular relationship exists between this protein and the plastoquinone/-plastoquinol pool, suggesting that its accumulation during cold acclimation is dependent on the redox state of the electron transport chain components (Dal Bosco et al, 2003). However, Crosatti et al (2008) showed that accumulation of COR14b is not adjusted through developmental regulation and was independent of plant stage or genotype. Among the Cor protein families, dehydrin proteins are partially characterized in barley.…”