Effects of growth substances on rice seedlings grown from seeds irradiated with gamma rays

El-Aishy, S. M.; Abd-Alla, S.; El-Keredy, M. S.

doi:10.1016/0098-8472(76)90035-6

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…According to Datta 27 , the reduction of plant height could be due to the damage to plant tissues which induced the inhibitory effect on the exposed plants. This observation was also reported in rice seedlings by El-Aishy, et al 28 . The rice seedlings exhibited an increased number of stunted roots after being exposed to high doses of gamma radiation, due to alterations in its genome 28 .…”

Section: Discussionsupporting

confidence: 85%

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Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Raffi

Rosli

et al. 2023

Sci Rep

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Due to their sessile nature, plants are exposed to various environmental stressors such as exposure to high levels of harmful ultraviolet (UV), ionizing, and non-ionizing radiations. This exposure may result in various damages, ranging from DNA and chromosomal aberrations to phenotypic abnormalities. As an adaptation, plants have evolved efficient DNA repair mechanisms to detect and repair any damage caused by exposure to these harmful stressors to ensure their survival. In this study, the effects of gamma radiation (as a source of ionizing radiation) on clonal Ananas comosus var. MD2 was evaluated. The morphology and physiology of the clonal plantlets before and after exposure to gamma radiation were monitored at specific time intervals. The degree of genetic variation between the samples pre- and post-irradiation was also analyzed by using inter-simple sequence repeat (ISSR) markers. The resulting data revealed that the heights of the irradiated plantlets were significantly reduced (compared to control), but improved with the recovery period. Irradiated samples also exhibited relatively good photosynthetic efficiency that further improved as the plantlets recover. These observations were supported by the ISSR analysis, where the genetic dissimilarities between the irradiated samples and control were reduced by 0.1017, after 4 weeks of recovery. Overall, our findings suggested that the phenotype recovery of the clonal A. comosus var. MD2 plantlets was contributed by their ability to detect and repair the DNA lesions (as exemplified by the reduction in genetic dissimilarity after 4 weeks) and hence allow the plantlets to undergo phenotype reversion to normal plant stature.

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Section: Discussionsupporting

confidence: 85%

“…This observation was also reported in rice seedlings by El-Aishy, et al 28 . The rice seedlings exhibited an increased number of stunted roots after being exposed to high doses of gamma radiation, due to alterations in its genome 28 . Analysis of physiological data in the current study further supported these observations.…”

Section: Discussionsupporting

confidence: 85%

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Raffi

Rosli

et al. 2023

Sci Rep

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“…X-ray stimulation of certain characters in some varieties at certain specific levels of irradiation in the present study may be due to the growth factor formed after irradiation (Moutschen 1958) or due to the increase cell size and/or cell divi sion or combined action of both (EL-Aishy et al 1976).…”

Section: Discussionmentioning

confidence: 89%

Radiosensitivity of the excised rice embryo.

Shome

Bhaduri

1980

CYTOLOGIA

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Hitherto, whole seeds have been mostly used for radiosensitivity studies and comparatively less attention has been paid on the effect of radiation on plant em bryos. When embryos in developing plant are exposed to radiation, they showed a greater amount of radiosensitivity than detached seed. These differences were in regard to germination, seedling lethality, growth and development, R, fertility and morphological anomalies (Mericle and Mericle 1957, Chatterjee et al. 1965, Kawai and Inoshita 1965.It is evident from the published literature that except the authors work (Shome 1966, Bhaduri and Shome 1969), till recently, no work has been done to study the radiosensitivity responses of excised rice embryos. In the present paper an attempt has been made to study the relative radiosensitivity responses of different varieties of rice embryos following embryo culture technique. Materials and methodsPureline seeds of rice (Oryza sativa L.)-'day-neutral' vars. Satika (early) and Ashkhata (late); 'short-day' vars. Badkalamkati-65 (early), Kumargore (late) and Kerangserang (late, Javanese rice) were used in this experiment. Excision of mature embryoDecoated rice grains were superficially sterilized with 95% alcohol for 1min followed by 1min in a 0.1% mercuric chloride solution. Grains were then rinsed throughly in sterile distilled water. Afterwards the sterilized grains were soaked in sterile distilled water for 3 to 4hr (in the case of excised embryos, soaking is ab solutely necessary). After removing the pericarp with a cataract knife, under a dis secting microscope, the whitish embryo becomes distinctly visible. Light pressure with the cataract knife between the boundary zone of embryo and endosperm was enough to free the embryo from the endosperm. Due caution was given during dissection so that the embryos were neither injured nor any endosperm tissue was kept adhering to the dissected embryo. Excision of immature embryoThe apiculus and the lower portion of the marked immature spikelet were care 1

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“…cirradiation induces various physiological and biochemical alterations in plants (Seung et al 2007). Changes in carbohydrate metabolism as affected by c-irradiation were shown to be a function of photosynthesis (El-Fiki et al 2003) and phytohormones (El-Aishy et al 1976). The extent of plant responsiveness to irradiation varied with species/cultivar and the dose of irradiation (Kim et al 2002).…”

Section: Introductionmentioning

confidence: 97%

Physiological effects of glycinebetaine on gamma-irradiated stressed fenugreek plants

Moussa

Jaleel

2010

Acta Physiol Plant

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Irradiation stress adversely affects plant growth and development. The radio protective activity by glycinebetaine in plants has not been reported, and its mechanism has not been known. Gamma rays at doses 0.0, 25, 50, 100, and 150 Gray (Gy) when applied pre-sowingly to dry seeds of fenugreek from a cobalt source ( 60 Co) with strength of 500 Ci and the dose rate of 0.54 Gy/min significantly reduced chlorophyll content, total protein, photosynthetic efficiency ( 14 CO 2 -fixation), total dry weight, the accumulation of reducing, non-reducing and total soluble sugars in comparison with un-irradiated control. It also significantly repressed the activities of hydrolytic enzymes (a-amylase and invertase), the carboxylating enzyme (ribulose-1,5-bisphosphate-carboxylase/oxygenase) in the developed fenugreek plants. Soaking irradiated seeds with glycinebetaine (50 mM) for 24 h partially alleviated the depression effects of irradiation in these parameters. c-irradiation treatment increased significantly H 2 O 2 content. Presoaking irradiated seeds with GB decreased significantly the H 2 O 2 level. The magnitude of the reversal decreased with increasing the irradiation dose. c-irradiation induced a significant decrease in the level of nucleic acids (DNA and RNA) accompanied by a corresponding induction of hydrolytic activities of DNase and RNase in the developed plants in comparison with un-irradiated control. Those changes were more significant at higher c-ray doses. Posttreatment of irradiated seeds with GB partially alleviated the adverse effects of radiation. It significantly increased nucleic acid levels and repressed the activities of DNase and RNase. The protective role played by glycinebetaine was more significant at lower c-ray doses. Pretreatment of seeds with GB may play an effective role in the radiorepair mechanism.

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Effects of growth substances on rice seedlings grown from seeds irradiated with gamma rays

Cited by 8 publications

References 12 publications

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Genetic and phenotype recovery of Ananas comosus var. MD2 in response to ionizing radiation

Radiosensitivity of the excised rice embryo.

Physiological effects of glycinebetaine on gamma-irradiated stressed fenugreek plants

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