Effects of Guanidinoacetic Acid, Creatine, and Choline on Protein Deposition and Creatine Status in Growing Cattle

Grant, Madeline S; Miesner, Matt D.; Titgemeyer, Evan C.

doi:10.4148/2378-5977.8032

Cited by 2 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Ardalan et al (2021) observed no change in N retention when GAA was supplemented to steers extremely deficient in Met, but a tendency ( P = 0.10) for GAA to increase N retention was observed when 6 g/d Met was provided to steers. Using a research model similar to the current experiment, Grant et al (2021) observed increases in N retention when 15 g/d GAA was abomasally supplemented to growing steers, whereas Grant et al (2022) observed that abomasal infusion of 15 g/d GAA did not affect N retention. The similar nature of our study and those of Grant et al (2021 , 2022 ) makes the different responses hard to explain, although the improvements observed by Grant et al (2021) were not extraordinarily large.…”

Section: Resultsmentioning

confidence: 50%

“…Using a research model similar to the current experiment, Grant et al (2021) observed increases in N retention when 15 g/d GAA was abomasally supplemented to growing steers, whereas Grant et al (2022) observed that abomasal infusion of 15 g/d GAA did not affect N retention. The similar nature of our study and those of Grant et al (2021 , 2022 ) makes the different responses hard to explain, although the improvements observed by Grant et al (2021) were not extraordinarily large. Several studies have evaluated the effect of feeding GAA on growth performance of finishing Angus bulls.…”

Section: Resultsmentioning

confidence: 50%

See 1 more Smart Citation

Effect of guanidinoacetic acid supplementation on nitrogen retention and methionine methyl group flux in growing steers fed corn-based diets

Speer

Grant

Miesner

et al. 2022

Journal of Animal Science

View full text Add to dashboard Cite

Six ruminally cannulated Holstein steers (256 ± 14 kg) were used in a 6 × 6 Latin square design to assess effects of guanidinoacetic acid (GAA) supplementation on N retention and methionine (Met) methyl group flux in growing cattle fed corn-based diets. Factorial treatments were 2 levels of Met (0 or 5 g/d) and 3 levels of GAA (0, 7.5, or 15 g/d) delivered by continuous abomasal infusion. Periods were 10 d in length and included 6 d of treatment adaptation, 3 d for total fecal and urine collections, and 1 d for blood sampling and flux measurements. Urinary N linearly increased (P < 0.01) with GAA supplementation and decreased (P < 0.05) with Met supplementation. Fecal N excretion was unaffected (P ≥ 0.42) by treatment. Retained N was not affected by GAA supplementation, but it was increased (P < 0.01) by Met supplementation. Use of methionine for transmethylation reactions, as well remethylation of homocysteine, was not affected by GAA supplementation when Met was not supplemented, but tended to be linearly increased by GAA supplementation when Met was supplemented (GAA-linear × Met interaction; P = 0.07), with the increases matching the amount of GAA provided. This response suggests that methylation reactions for compounds other than GAA were reduced by GAA supplementation when Met supply was deficient. Plasma concentrations and urinary excretion of creatine increased linearly (P = 0.03 and P = 0.06, respectively) when GAA was supplemented. There was a linear increase (P < 0.01) in urinary GAA excretion with GAA supplementation. Neither plasma concentration nor urinary excretion of creatinine was affected (P ≥ 0.17) by treatment. No treatment differences (P ≥ 0.13) were observed for plasma haptoglobin concentrations. Plasma urea-N linearly increased (P < 0.05) with GAA supplementation. Concentrations of Met and taurine increased (P < 0.05) when Met was supplemented. Plasma arginine was greatest at the intermediate level of supplemental GAA (quadratic, P < 0.05). The increase in N retention when Met was supplemented demonstrates Met was limiting in the corn-based diet. Supplementation of GAA alone or with Met as a methyl donor did not increase N retention in growing steers, perhaps because creatine production was favored over protein deposition as a use for Met.

show abstract