Effects of halothane and colchicine on microtubules and electrical activity of rabbit vagus nerves

Hinkley, Robert E.; Green, Louis S.

doi:10.1002/neu.480020202

Cited by 52 publications

(13 citation statements)

References 13 publications

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“…One could argue that the Fluo-DLT accumulation observed here at the level of DRG had, in fact, resulted from retrograde transport of Fluo-DLT molecules internalized by presynaptic afferent axons in the dorsal horn of the spinal cord. This was not the case, however, because rats injected intrathecally with colchicine, at doses shown previously to efficiently inhibit axonal transport (Hinkley and Green, 1971;Tohda et al, 2001;Lee et al, 2002) without affecting opioid receptor internalization (Lee et al, 2002), and found here to greatly reduce ␤-tubulin immunostaining, failed to show a significant difference in the intraperikaryal accumulation of Fluo-DLT.…”

Section: Discussioncontrasting

confidence: 71%

“…To test whether -Bodipy 576/589 deltorphin-I 5-aminopentylamide (Fluo-DLT) was transported axonally, in vivo internalization experiments were repeated in rats (n ϭ 3) injected intrathecally with a saline solution (30 l) containing 0.2 mg colchicine 16 h earlier (Hinkley and Green, 1971). Control rats (n ϭ 3) were injected intrathecally with saline.…”

Section: Methodsmentioning

confidence: 99%

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Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Gendron¹,

Lucido²,

Mennicken³

et al. 2006

J. Neurosci.

140

157

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The present study demonstrates that perikaryal ␦-opioid receptors (␦ORs) in rat dorsal root ganglion (DRG) neurons bind and internalize opioid ligands circulating in the CSF. Using confocal and electron microscopy, we found that prolonged morphine treatment increased the cell surface density of these perikaryal ␦ORs and, by way of consequence, receptor-mediated internalization of the fluorescent deltorphin (DLT) analog -Bodipy 576/589 deltorphin-I 5-aminopentylamide (Fluo-DLT) in all three types of DRG neurons (small, medium, and large). In contrast, chronic inflammatory pain induced by the injection of complete Freund's adjuvant (CFA) into one hindpaw selectively increased Fluo-DLT internalization in small and medium-sized DRG neurons ipsilateral to the inflammation. Based on our previous studies in the spinal cord of -opioid receptor (OR) knock-out mice, it may be assumed that the enhanced membrane recruitment of ␦ORs observed after sustained morphine is attributable to stimulation of ORs. However, the selectivity of the effect induced by inflammatory pain suggests that it involves a different mechanism, namely a modality-specific and pain-related activation of C and A␦ fibers. Indeed, stimulation by capsaicin of transient receptor potential vanilloid 1 receptors, which are selectively expressed by small diameter (Ͻ 600 m 2 ) DRG neurons, increased Fluo-DLT internalization exclusively in this cell population. The present results, therefore, demonstrate that DRG neurons express perikaryal ␦ORs accessible to CSF-circulating ligands and that the density and, hence, presumably also the responsiveness, of these receptors may be modulated by both pain-related stimuli and sustained exposure to OR agonists.

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Section: Discussioncontrasting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Gendron¹,

Lucido²,

Mennicken³

et al. 2006

J. Neurosci.

140

157

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“…A detailed electrophysiological characterization and demonstration that this effect is a long-lasting synaptic change which persists after unloading the insect have been previously reported (Donoso and Luco, 1973;Luco, 1978). In addition, the present study shows that the increase in the probability of synaptic transmission was reversibly inhibited by colchicine treatment, without altering the normal propagation of action potentials along the nerve cord, the latter result being entirely consistent with that indicating that colchicine, at the concentration used, does not affect nerve conduction in the cockroach nerve cord (Donoso and Fernandez, 1973) or in other preparations (Hinkley and Green, 1971;Inestrosa and Fernandez, 1976). In view of the demonstration that colchicine blocked axonal transport and temporarily prevented the arrival of labeled proteins at the metathoracic ganglion, we conclude that inhibition of the synaptic modification studied is a direct consequence of the inhibitory effect of the drug on axonal transport.…”

Section: Effect Of Colchicine O N Synaptic Responsessupporting

confidence: 93%

Axonal transport involvement in long‐lasting synaptic modifications in blatta orientalis

Fernández

Donoso

1983

J. Neurobiol.

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To determine whether axonal transport plays a role in the establishment of long-lasting changes in synaptic transmission, the effects of colchicine on transport and on synaptic modifications induced by hyperactivity were studied in the nerve cord of the cockroach Blatta orientalis. Application of a lead weight on the insect's dorsum, and the consequent exaggerated use of antigravity reflexes, facilitated synaptic transmission along a particular nervous pathway in the metathoracic ganglion. Application of colchicine in the prothoracic ganglion reversibly blocked such synaptic facilitation and temporarily interfered with the transport of proteins along the cord. Five components of axonal transport, moving at 2, 10, 25, 75, and 150 mm/day, were altered by colchicine treatment with a temporal course that coincided with the reversible inhibition of synaptic facilitation. These results were brought about by colchicine acting directly on axonal transport at the level of the prothoracic ganglion, rather than on synaptic transmission measured at the metathoracic ganglion. The temporal correlation observed between the effects of colchicine on axonal transport and on synaptic facilitation strongly suggest that the transport process is essential for long-lasting synaptic modifications to take place.

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“…Previously, we showed that halothane (Fluothane ; Ayerst Laboratories, New York) in appropriate concentrations increases the number of formed microtubules in isolated rabbit vagus nerves (4) . This observation and those of Seeds et al .…”

Section: Introductionmentioning

confidence: 99%

Anesthetic-Induced Transformation of Axonal Microtubules

Hinkley

Samson

1972

The Journal of Cell Biology

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Effects of halothane and colchicine on microtubules and electrical activity of rabbit vagus nerves

Cited by 52 publications

References 13 publications

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Axonal transport involvement in long‐lasting synaptic modifications in blatta orientalis

Anesthetic-Induced Transformation of Axonal Microtubules

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