Effects of hCG and salmon gonadoliberine analogue on spermiation in the Eurasian perch ( Perca fluviatilis )

Żarski, Daniel; Bernáth, Gergely; Król, Jarosław; Cejko, Beata Irena; Bokor, Zoltán; Palińska‐Żarska, Katarzyna; Milla, Sylvain; Fontaine, Pascal; Krejszeff, Sławomir

doi:10.1016/j.theriogenology.2017.08.022

Cited by 28 publications

(14 citation statements)

References 29 publications

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“…Cells were activated with an ionic solution (45 mM NaCl, 5 mM KCl, 30 mM Tris, pH: 8.0 ± 0.2) designed for common carp (Saad, Billard, Theron, & Hollebecq, ). Motility parameters, such as progressive motility (criteria according to the Sperm VisionTM v. 3.7.4.‐straight line distance >5 µm, pixel to µm ratio: 151–100, pMOT %), curvilinear velocity (VCL, µm/s), straight linear velocity (VSL, μm/s), linearity of movement (LIN, %), amplitude of lateral head displacement (ALH, μm), beat cross frequency (BCF, Hz) were chosen in to characterize carp sperm movement (Żarski et al, ). Measurement was carried out at least in duplicates where moving cells were identified (1–100 µm 2 ) with a digital camera (JAI CV‐A10 CL; Minitube of America) using a frame rate of 60 s −1 .…”

Section: Methodsmentioning

confidence: 99%

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Várkonyi

Bokor

Molnár

et al. 2018

Reprod Domestic Animals

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Contents In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large‐scale (elevated volume of sperm) freezing method in a controlled‐rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled‐rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

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Section: Methodsmentioning

confidence: 99%

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Várkonyi

Bokor

Molnár

et al. 2018

Reprod Domestic Animals

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“…To promote and synchronize the spawning act of both sexes, fish were hormonally stimulated using salmon gonadoliberin analog (sGnRHa, BACHEM, Switzerland) (injection at a dose of 50 μg kg −1 ) 53 . Sperm were collected 7 days post hormonal stimulation (which was within the optimal period of sperm collection of this species) 54 , whereas eggs were collected between 3- and 5-days following injection depending on the maturation stage of the females 55 . Prior to any manipulation, such as gamete collection, the fish were anesthetized in MS-222 (Argent, USA) at a dose of 150 mg L −1 .…”

Section: Discussionmentioning

confidence: 99%

Paternal-effect genes revealed through semen cryopreservation inPerca fluviatilis

Panda,

Judycka,

Palińska-Żarska

et al. 2023

Preprint

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Knowledge about paternal-effect genes (the expression of which in progeny is controlled by the paternal genome) in fish is very limited. To explore this issue, we used semen cryopreservation as a specific challenge test for sperm cells, thus enabling selection amidst cryo-sensitivity. We created two groups of Eurasian perch (Perca fluviatilis) as a model – eggs fertilized either with fresh (Fresh group) or cryopreserved (Cryo group) semen from the same male followed by zootechnical-transcriptomic examination of consequences of cryopreservation in obtained progeny (at larval stages). Most of the zootechnical observations were similar in both groups, except the final weight was higher in the Cryo group. Semen cryopreservation appeared to act as a “positive selection” factor, upregulating most paternal-effect genes in the Cryo group. Transcriptomics profile of freshly hatched larvae sourced genes involved in the development of visual perception as paternal-effect genes. Consequently, larvae from the Cryo group exhibited enhanced eyesight, potentially contributing to more efficient foraging and weight gain compared to the Fresh group. This study unveils, for the first time, the significant influence of the paternal genome on the development of the visual system in fish, highlightingpde6g,opn1lw1, andrbp4las novel paternal-effect genes.

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“…The use of the hCG hormone as a spawning inducer has been used in the last decades in tilapia mainly with the purpose of obtaining gametes for in vitro fertilization, through the extrusion of breeders (Alvarenga et al, 2020; Carrillo & Romagosa, 2004; El‐Gamal & El‐Greisy, 2005; Fernandes et al, 2013; Garcia‐Abiado et al, 1994; Guzmán et al, 2011; Owusu‐Frimpong, 2008; Senthilkumaran et al, 2002; Souza et al, 2016; Yoshiura et al, 2003; Zarski et al, 2017). The hCG has LH‐like activity and stimulates the final oocyte maturation (Mylonas et al, 2010) through the induction of ultrastructural and molecular changes in ovarian follicles (Patiño & Thomas, 1990; York et al, 1993) and steroid production.…”

Section: Discussionmentioning

confidence: 99%

Use of hCG hormone in the natural and artificial reproduction of Nile tilapia ( Oreochromis niloticus )

et al. 2021

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This study aimed to develop a protocol to synchronize final oocyte maturation and oocyte collection in both natural (first experiment) and artificial reproduction (second experiment) of Nile tilapia. In the first experiment, 80 young females (1 year old), 40 old females (3 years old) and 75 young males were used and splitted into three different groups. Each group bred once by natural mating during 7 days. Each female received an injection of hCG (3 IU/g of female) or saline solution (control) just before being stocked with males. In the second experiment, we evaluated different doses (0.5, 1.5, 2.5 and 3.5 IU/g female), number of injections (1 or 2) and administration route (intracoelomic or intramuscular) of hCG hormone in 40 females for artificial reproduction by stripping. The use of hCG (3 IU/g of female) was not effective for synchronization of natural reproduction in Nile tilapia, and the negative effect of age on reproductive performance was not reversed by its use. In the second assay, the use of a single injection with lower doses of hCG (0.5 and 1.5 IU/g of female) resulted in 100% of spawned females in artificial reproduction.

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Effects of hCG and salmon gonadoliberine analogue on spermiation in the Eurasian perch ( Perca fluviatilis )

Cited by 28 publications

References 29 publications

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Paternal-effect genes revealed through semen cryopreservation inPerca fluviatilis

Use of hCG hormone in the natural and artificial reproduction of Nile tilapia ( Oreochromis niloticus )

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