Effects of heat-shock and inhibitors of protein synthesis on irreversible membrane damage occurring during the hypersensitive reaction of Lactuca sativa L. to Bremia lactucae Regel

Woods, Alison M.; Fagg, Jean; Mansfıeld, John W.

doi:10.1016/0885-5765(89)90077-5

Cited by 16 publications

(4 citation statements)

References 22 publications

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“…1988). Woods et al 1989, in their experiments to investigate the effect of heat-shock on IMD occurring during the HR reaction of Lactuca sativa L. to infection of Bremia lactucae Regel demonstrated the application of heat shock would affect the protein synthesis by inhibiting the translation of mRNA other than those newly synthesis. The temporary suppression of the protein synthesis results in delay both the occurrence of IMD and also the onset of HR reaction.…”

Section: Accumulation Of Lettucenin a After Different Abiotic Elicitamentioning

confidence: 99%

Aging Effect to Accumulation of Lettucenin A in Lettuce after Elicitation with Various Abiotic Elicitors

Ong¹,

Chong²

2009

MAS

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Aging Effect to Accumulation of Lettucenin A in Lettuce after Elicitation with Various Abiotic Elicitors

Ong¹,

Chong²

2009

MAS

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Localization of Hydrogen Peroxide Accumulation during the Hypersensitive Reaction of Lettuce Cells to Pseudomonas syringae pv phaseolicola

Bestwick¹,

Brown²,

Bennett³

et al. 1997

The Plant Cell

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The active oxygen species hydrogen peroxide (H2O2) was detected cytochemically by its reaction with cerium chloride to produce electron-dense deposits of cerium perhydroxides. In uninoculated lettuce leaves, H2O2 was typically present within the secondary thickened walls of xylem vessels. Inoculation with wild-type cells of Pseudomonas syringae pv phaseolicola caused a rapid hypersensitive reaction (HR) during which highly localized accumulation of H2O2 was found in plant cell walls adjacent to attached bacteria. Quantitative analysis indicated a prolonged burst of H2O2 occurring between 5 to 8 hr after inoculation in cells undergoing the HR during this example of non-host resistance. Cell wall alterations and papilla deposition, which occurred in response to both the wild-type strain and a nonpathogenic hrpD mutant, were not associated with intense staining for H2O2, unless the responding cell was undergoing the HR. Catalase treatment to decompose H2O2 almost entirely eliminated staining, but 3-amino-1,2,4-triazole (catalase inhibitor) did not affect the pattern of distribution of H2O2 detected. H2O2 production was reduced more by the inhibition of plant peroxidases (with potassium cyanide and sodium azide) than by inhibition of neutrophil-like NADPH oxidase (with diphenylene iodonium chloride). Results suggest that CeCl3 reacts with excess H2O2 that is not rapidly metabolized during cross-linking reactions occurring in cell walls; such an excess of H2O2 in the early stages of the plant-bacterium interaction was only produced during the HR. The highly localized accumulation of H2O2 is consistent with its direct role as an antimicrobial agent and as the cause of localized membrane damage at sites of bacterial attachment.

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“…In many plants, treatment with inhibitors of protein synthesis has been found to prevent or delay the HR, implying a requirement for active metabolism within the plant cell leading to cell collapse (Woods et al, 1989;He et al, 1993). The occurrence of lesion mimics (notably in Arabidopsis), which rapidly form HR-like lesions in the absence of an avirulent pathogen, suggests that the HR is under genetic control.…”

Section: Introductionmentioning

confidence: 99%

“…These include a neutrophil-analogous, membrane-bound NADPH oxidase (Doke, 1983; Levine et al, 1994; Auh and Murphy, 1995; Doke and Miura, 1995; Dwyer et al, 1996), lipoxygenase (Croft et al, 1990), and apoplastic peroxidases (Vera-Estrela et al, 1992;Bolwell et al, 1995). Adam et al (1 995) have suggested that H202 accumulation may occur by a reduction in H202 scavenging and an increase in H,OZ production involving coordinated changes in peroxidase, superoxide dismutase, and catalase activities.In many plants, treatment with inhibitors of protein synthesis has been found to prevent or delay the HR, implying a requirement for active metabolism within the plant cell leading to cell collapse (Woods et al, 1989; He et al, 1993). The occurrence of lesion mimics (notably in Arabidopsis), which rapidly form HR-like lesions in the absence of an avirulent pathogen, suggests that the HR is under genetic control.…”

mentioning

confidence: 99%

Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola.

et al. 1997

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The active oxygen species hydrogen peroxide (H202) was detected cytochemically by its reaction with cerium chloride to produce electron-dense deposits of cerium perhydroxides. In uninoculated lettuce leaves, H202 was typically present within the secondary thickened walls of xylem vessels. Inoculation with wild-type cells of Pseudomonas syringae pv phaseolicola caused a rapid hypersensitive reaction (HR) during which highly localized accumulation of H202 was found in plant cell walls adjacent to attached bacteria. Quantitative analysis indicated a prolonged burst of H202 occurring between 5 to 8 hr after inoculation in cells undergoing the HR during this example of non-host resistance. Cell wall alterations and papilla deposition, which occurred in response to both the wild-type strain and a nonpathogenic hrpD mutant, were not associated with intense staining for H202, unless the responding cell was undergoing the HR. Catalase treatment to decompose H, O, almost entirely eliminated staining, but 3-amino-l,2,4-triazole (catalase inhibitor) did not affect the pattern of distribution of H202 detected. H202 production was reduced more by the inhibition of plant peroxidases (with potassium cyanide and sodium azide) than by inhibition of neutrophil-like NADPH oxidase (with diphenylene iodonium chloride). Results suggest that CeCI, reacts with excess H202 that is not rapidly metabolized during cross-linking reactions occurring in cell walls; such an excess of H202 in the early stages of the plant-bacterium interaction was only produced during the HR. The highly localized accumulation of H,Oz is consistent with its direct role as an antimicrobial agent and as the cause of localized membrane damage at sites of bacterial attachment. INTRODUCTIONThe active resistance of plants to colonization by bacteria and fungi is often expressed by the hypersensitive reaction (HR) of challenged plant cells (Ingram, 1978; Klement, 1982; Mansfield, 1990; Tenhaken et al., 1995). The HR can be recognized as the rapid and localized death of cells in response to an avirulent pathogen; it has been observed during most interactions involving race-specific resistance and also in many examples of non-host resistance (Heath, 1989; Mansfield, 1990; Mansfield et al., 1997). A second form of resistance, more commonly found in non-host reactions, is the highly localized alteration of the cell wall at sites attacked by fungi or bacteria. Modification of the cell wall per se is often associated with the formation of a papilla or apposition at reaction sites (Ride, 1986; Nicholson and Hammerschmidt, 1992; Bestwick et al., 1995). In phytopathogenic bacteria, hypersensitive response and pathogenicity (hrp) genes determine the ability to multiply within susceptible plants and to cause Current address: Division of Biochemical Sciences, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK. To whom correspondence should be addressed. a macroscopic HR in either resistant varieties of their host or in non-host plants (Bonas, 1994). Hrp ...

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Effects of heat-shock and inhibitors of protein synthesis on irreversible membrane damage occurring during the hypersensitive reaction of Lactuca sativa L. to Bremia lactucae Regel

Cited by 16 publications

References 22 publications

Aging Effect to Accumulation of Lettucenin A in Lettuce after Elicitation with Various Abiotic Elicitors

Aging Effect to Accumulation of Lettucenin A in Lettuce after Elicitation with Various Abiotic Elicitors

Localization of Hydrogen Peroxide Accumulation during the Hypersensitive Reaction of Lettuce Cells to Pseudomonas syringae pv phaseolicola

Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola.

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