Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Tonomura, Hitoshi; Takahashi, Kenji; Mazda, Osam; Shin‐Ya, Masaharu; Honjo, Kuniaki; Hojo, Tatsuya; Imanishi, Jirô; Kubo, Toshikazu

doi:10.1002/jor.20421

Cited by 39 publications

(47 citation statements)

References 26 publications

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“…Heat stimulation regulates T cell proliferation and activation, promotes the priming and activation of dendritic cells, and enhances the migration of Langerhans cells from the epidermis to lymph nodes [9]. It also increases matrix production at cartilage [10].…”

Section: Introductionmentioning

confidence: 99%

Heat stimulation reduces early adipogenesis in 3T3-L1 preadipocytes

Ezure¹,

Amano²

2009

Endocr

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In this study, we used 3T3-L1 preadipocytes as a model to investigate the effects of heat stimulation on adipogenesis, which is a key process in the development of obesity. Heat stimulation at 43 degrees C for 60 min significantly reduced lipid accumulation, as measured by Oil Red-O assay. In the early phase of adipogenesis, heat stimulation almost completely blocked the increase of CCAAT/enhancer binding protein delta (C/EBPdelta) gene expression and delayed the onset of the increase of C/EBPbeta gene expression. The expression of proliferator-activated receptor gamma (PPARgamma), which is regulated by these factors, was also reduced. In the later phase of adipogenesis, the induction of adipocyte-specific genes, such as C/EBPalpha, adipocyte protein 2 (aP2), lipoprotein lipase (LPL), adiponectin, and glucose transporter 4 (Glut4), which are regulated by PPARgamma, was reduced. However, adipogenesis was not significantly reduced if heat stimulation was carried out after the early phase of adipogenesis. These results suggest that heat stimulation reduces adipogenesis by decreasing the expression of adipogenesis-related transcriptional factors during early adipogenesis.

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Section: Introductionmentioning

confidence: 99%

Heat stimulation reduces early adipogenesis in 3T3-L1 preadipocytes

Ezure¹,

Amano²

2009

Endocr

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“…1,4 In this study, PG was significantly induced by MES þ HS, an effect thought to occur via HSP70. Although col2 increased with HS by microwave without the involvement of HSP70 expression, 1 we found that col2 was not affected by MES þ HS. The differences in the HS method (microwave and heat conduction) might influence the expression of col2.…”

Section: Discussionmentioning

confidence: 55%

“…In recent years, the effectiveness of several drugs, such as chondroitin sulfate and glucosamine, has been reported, but these supplements cannot completely halt the progression of OA. 1 Thus, it is necessary to develop an efficient, safe, and inexpensive therapeutic modality that can prevent cartilage degeneration at the initial stages of OA.…”

mentioning

confidence: 99%

Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte

Hiraoka

Takahashi

Mazda

et al. 2013

Journal Orthopaedic Research

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The objective of this study is to investigate the effects of mild electrical stimulation (MES) and heat stress (HS) on heat shock protein 70 (HSP70), that protects chondrocytes and enhances cartilage matrix metabolism, in chondrocyte and articular cartilage. Rabbit articular chondrocytes were treated with MES and/or HS. The safeness was assessed by LDH assay and morphology. HSP70 protein, ubiquitinated proteins and HSP70 mRNA were examined by Western blotting and real-time PCR. Rat knee joints were treated with MES and/or HS. HSP70 protein, ubiquitinated proteins, HSP70 mRNA and proteoglycan core protein (PG) mRNA in articular cartilage were investigated. In vitro, HS increased HSP70 mRNA and HSP70 protein. MES augmented ubiquitinated protein and HSP70 protein, but not HSP70 mRNA. MES þ HS raised HSP70 mRNA and ubiquitinated protein, and significantly increased HSP70 protein. In vivo, HS and MES þ HS treatment augmented HSP70 mRNA. HS modestly augmented HSP70 protein. MES þ HS significantly increased HSP70 protein and ubiquitinated proteins. PG mRNA was markedly raised by MES þ HS. This study demonstrated that MES, in combination with HS, increases HSP70 protein in chondrocytes and articular cartilage, and promotes cartilage matrix metabolism in articular cartilage. MES in combination with HS can be a novel physical therapy for osteoarthritis by inducing HSP70 in articular cartilage. ß

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“…This suggests that stimulation at 60 W induces excess stress in chondrocytes, from which cells cannot recover, and that HSP70 serves primarily to protect chondrocytes rather than to promote aggrecan expression. 9 Stimulation at 40 W was therefore assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. In the present study, the intraarticular temperature just after heating at 40 W was 38.38C.…”

Section: Discussionmentioning

confidence: 99%

“…While excessive heat stimulation (438C or greater) induced HSP70 expression higher, metabolic activity decreased. 9 MW-induced HSP70 expression therapy at appropriate intensities may therefore be effective.…”

mentioning

confidence: 99%

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

Fujita

Nakagawa

Takahashi

et al. 2011

Journal Orthopaedic Research

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The objective of the present study was to investigate the effects of heat stimulation and glutamine (Gln) on the expression of extracellular matrix genes and heat shock protein 70 (HSP70) in rat articular cartilage in vivo and to determine whether HSP70 expression achieved with a combination of microwave (MW) and Gln suppresses osteoarthritis (OA) progression in a rat OA model. Stimulation at 40 W was assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. Articular cartilage was collected at 8 h after heat stimulation and/or intraarticular Gln administration, and total RNA was extracted. The expression of HSP70, aggrecan, and type II collagen was quantified using real-time RT-PCR. Cartilage samples from the OA model were subjected to hematoxylin and eosin (HE) and safranin O staining. HSP70 and aggrecan expression was greatest in a group receiving both MW and Gln. In the rat OA model, the severity of OA was significantly milder in a group receiving MW and Gln than in the control group. HSP70, stimulated by the combination of MW heat and Gln, may be involved in the suppression of OA progression. ß

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Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Cited by 39 publications

References 26 publications

Heat stimulation reduces early adipogenesis in 3T3-L1 preadipocytes

Heat stimulation reduces early adipogenesis in 3T3-L1 preadipocytes

Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

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