Effects of heat stress on conception in Holstein and Jersey cattle
                    and oocyte maturation in vitro

Lee, Ji‐Hwan; Kim, Doo-San; Choi, Inchul; Kim, Donghyeon; Son, Jun-Kyu; Jeon, Eunjeong; Jung, Dajinsol; Han, Manhye; Ha, Seungmin; Hwang, Seongsoo

doi:10.5187/jast.2022.e113

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Denuded oocytes were frozen at −80 °C for later analysis of ATP. ATP content was assessed in individual embryos using the ATP Determination kit (Invitrogen™/Life Technologies/Thermo Fisher Scientific) [ 59 ]. A six-point standard curve (0–5 pmol) was considered in each test series.…”

Section: Methodsmentioning

confidence: 99%

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Wrzecińska

Kowalczyk

Kordan

et al. 2023

IJMS

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The main problem in dairy herds is reproductive disorders, which are influenced by many factors, including temperature. Heat stress reduces the quality of oocytes and their maturation through the influence of, e.g., mitochondrial function. Mitochondria are crucial during oocyte maturation as well as the process of fertilization and embryonic development. Disturbances related to high temperature will be increasingly observed due to global warming. In present studies, we have proven that exposure to high temperatures during the cleaving of embryos statistically significantly (at the level of p < 0.01) reduces the percentage of oocytes that cleaved and developed into blastocysts eight days after insemination. The study showed the highest percentage of embryos that underwent division in the control group (38.3 °C). The value was 88.10 ± 6.20%, while the lowest was obtained in the study group at 41.0 °C (52.32 ± 8.40%). It was also shown that high temperature has a statistically significant (p < 0.01) effect on the percentage of embryos that developed from the one-cell stage to blastocysts. The study showed that exposure to a temperature of 41.0 °C significantly reduced the percentage of embryos that split relative to the control group (38.3 °C; 88.10 ± 6.20%). Moreover, it was noted that the highest tested temperature limits the development of oocytes to the blastocyst stage by 5.00 ± 9.12% compared to controls (33.33 ± 7.10%) and cleaved embryos to blastocysts by 3.52 ± 6.80%; the control was 39.47 ± 5.40%. There was also a highly significant (p < 0.0001) effect of temperature on cytoplasmic ROS levels after 6 and 12 h IVM. The highest level of mitochondrial ROS was found in the group of oocytes after 6 h IVM at 41.0 °C and the lowest was found in the control group. In turn, at 41.0 °C after 12 h of IVM, the mitochondrial ROS level had a 2.00 fluorescent ratio, and the lowest in the group was 38.3 °C (1.08). Moreover, with increasing temperature, a decrease in the expression level of both LC3 and SIRT1 protein markers was observed. It was proved that the autophagy process was impaired as a result of high temperature. Understanding of the cellular and molecular responses of oocytes to elevated temperatures will be helpful in the development of heat resistance strategies in dairy cattle.

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Section: Methodsmentioning

confidence: 99%

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Wrzecińska

Kowalczyk

Kordan

et al. 2023

IJMS

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show abstract

“…The mitochondrial distribution of the oocytes was assayed by staining with Mito-Tracker Red CMXRos (M7512, Invitrogen, Carlsbad, CA, USA), with a slight modification to the method of Lee [112]. After 22-24 h of maturation, the oocytes were placed in 1 mg/mL hyaluronidase solution to remove granulosa cells.…”

Section: Mitochondrial-distribution Examination Of Oocytesmentioning

confidence: 99%

Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience

Feng,

Li,

Zhang

et al. 2024

IJMS

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Extreme temperature during summer may lead to heat stress in cattle and compromise their productivity. It also poses detrimental impacts on the developmental capacity of bovine budding oocytes, which halt their fertility. To mitigate the adverse effects of heat stress, it is necessary to investigate the mechanisms through which it affects the developmental capacity of oocytes. The primary goal of this study was to investigate the impact of heat stress on the epigenetic modifications in bovine oocytes and embryos, as well as on oocyte developmental capacity, reactive oxygen species, mitochondrial membrane potential, apoptosis, transzonal projections, and gene expression levels. Our results showed that heat stress significantly reduced the expression levels of the epigenetic modifications from histone H1, histone H2A, histone H2B, histone H4, DNA methylation, and DNA hydroxymethylation at all stages of the oocyte and embryo. Similarly, heat stress significantly reduced cleavage rate, blastocyst rate, oocyte mitochondrial-membrane potential level, adenosine-triphosphate (ATP) level, mitochondrial DNA copy number, and transzonal projection level. It was also found that heat stress affected mitochondrial distribution in oocytes and significantly increased reactive oxygen species, apoptosis levels and mitochondrial autophagy levels. Our findings suggest that heat stress significantly impacts the expression levels of genes related to oocyte developmental ability, the cytoskeleton, mitochondrial function, and epigenetic modification, lowering their competence during the summer season.

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Heat stress effect on fertility of two imported dairy cattle breeds from different Algerian agro-ecological areas

Ferag,

Gherissi,

Khenenou

et al. 2024

Int J Biometeorol

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Effects of heat stress on conception in Holstein and Jersey cattle and oocyte maturation in vitro

Cited by 3 publications

References 33 publications

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization

Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience

Heat stress effect on fertility of two imported dairy cattle breeds from different Algerian agro-ecological areas

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