Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

Abstract

Many factors affect successful virus propagation and plant defence responses. Heat shock protein (Hsp) expression after heat shock plays an ambiguous role in viral infection. On the one hand, Hsp70 participates in plant defence response; on the other hand, Hsp70 could interact with viral proteins and facilitate virus propagation.• Here, we studied metabolic adaptations of Nicotiana tabacum L. subjected to heat shock (42°C, 2 h) before or after inoculating the plants with Potato virus Y (potyvirus). RT-qPCR and ELISA were used for potyvirus quantification. Hsp70 and Hsp90 isoforms were analysed by Western blotting. Salicylic, quinic and chlorogenic acid content was determined by LC-MS. The activity of Hatch-Slack enzymes (as markers of potyviral infection in tobacco) and glycosidases was assayed.• Application of heat shock before or after inoculation showed accelerated potyviral propagation in comparison with only inoculated plants. Plants exposed to heat shock and concurrently inoculated showed higher potyviral content, higher amount of Hsp70, together with late decline of quinic acid content and low chlorogenic acid content. Spread of potyviral infection correlated with enhanced salicylic acid content and activities of enzymes of the Hatch-Slack cycle, aand b-galactosidase, a-mannosidase, a-glucosidase and b-N-acetylhexosaminidase.• Heat shock proteins accelerate potyviral propagation. The lower weight cytosolic and mitochondrial Hsp70 (~50-75 kDa) persist throughout the viral infection. Also, the plant defense response results in increase of salicylic and chlorogenic acids but decrease of quinic acid content.

Modulation of Expression of PVYNTN RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars

“…They postulated that PVY 0 exerted no notable effects on the heat-stress response in resistant plants, whereas in susceptible hosts, PVY 0 infection induced the HSP signaling pathway and modulated the HSP response triggered by heat stress. Hýsková et al [90] observed that the application of heat shock before or after inoculation of the Petit Havana tobacco with PVY accelerated the propagation of infection.…”

Regulation of heat shock proteins 70 and their role in plant immunity

“…References: 1–2, Liebrand et al (2012 , 2014 ); 3, Nekrasov et al (2009) ; 4–5, Park et al (2010 , 2014 ); 6, Nagashima et al (2014) ; 7, Poór et al (2019) ; 8, Wang et al (2005) ; 9, Carvalho et al (2014) ; 10, Qiang et al (2012) ; 11, Xu et al (2012) ; 12, Kanzaki et al (2003) ; 13, Noël et al . (2007) ; 14, Thao et al (2007) ; 15, Nakashima et al (2008) ; 16, Shirasu (2009) ; 17, X.-C. Zhang et al (2015) ; 18, Jing et al (2016) ; 19, Zhou et al (2021) ; 20–21, Jelenska et al (2007 , 2010 ); 22, Lee et al (2018) ; 23, Kim and Hwang (2015) ; 24, Hýsková et al (2021b) ; 25, Ye et al (2011) ; 26, Černý et al . (2016) ; 27, Krishnakumar et al (2017) .…”

“…Similarly, a positive effect of HSP70s on viral replication was reported for Chinese wheat mosaic furovirus ( Yang et al , 2017 ) and Cowpea severe mosaic virus ( Paiva et al , 2016 ; Varela et al , 2017 ), and indicated for Tomato bushy stunt virus ( Pogany et al , 2008 ). Further, Nicotiana tabacum plants exposed to heat shock and inoculated with Potato virus Y showed higher amounts of HSP70 genes and higher virus content than the corresponding controls inoculated at standard temperature ( Hýsková et al , 2021b ).…”

“…References: 1, Song et al (2015) ; 2, Noël et al . (2007) ; 3, Lyu et al (2020) ; 4, Wu et al (2019) ; 5, Góngora-Castillo et al (2012) ; 6, Saiz-Fernández et al (2020) ; 7, Gupta et al (2017) ; 8, Klink et al (2007) ; 9, Berka et al (2020) ; 10, Molitor et al (2011) ; 11, Sharma Poudel et al (2019) ; 12, C. Zhang et al (2015) ; 13, Ye et al (2011) ; 14, Hafrén et al (2010) ; 15, Caplan et al (2009) ; 16, Hýsková et al (2021b) ; 17, Crampton et al (2009) ; 18, Cerna et al (2017) ; 19, Coelho et al (2021) ; 20, Singh et al (2019) ; 21, Vitale et al (2014) ; 22, Naveed and Ali (2018) ; 23, Gorovits et al (2013) ; 24, Chen et al (2013) ; 25, Pan et al (2013) ; 26, Szajko et al (2020) ; 27, Guo et al (2021) ; 28, Yang et al (2017) ; 29, Paiva et al (2016) ; 30, Varela et al (2017) ; 31, Liu et al (2021) ; 32, Chen et al (2017) .…”

Regulatory hubs in plant stress adaptation

“…Hýsková et al . (2021) found that heat shock and subsequent HSP70 expression (especially cytoplasmic and mitochondrial isoforms) accelerate the systemic propagation of viruses in plants by inducing metabolic changes in levels of phenolic acids, glycosides and enzymes of the Hatch‐Slack cycle.…”