Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarctica

Abdulrasheed, Mansur; Roslee, Ahmad Fareez Ahmad; Zakaria, Nur Nadhirah; Zulkharnain, Azham; Lee, Gillian Li Yin; Convey, Peter; Napis, Suhaimi; Ahmad, Siti Aqlima

doi:10.22438/jeb/41/5/mrn-1319

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…In a shake flask setting, pH changes are largely due to the accumulation of metabolic wastes, which can be compensated for through the use of suitable buffer systems. TPH degradation and microbial growth were both maximal in the current study between pH 7 and 7.5, consistent with similar studies [15,41,46].…”

Section: Discussionsupporting

confidence: 92%

Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica

Roslee

Gómez-Fuentes

Zakaria

et al. 2021

Biology

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Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g/L NH4Cl and 2.0% v/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10‒15 °C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g/L NH4Cl, 12.5 °C and 1.75% v/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, μmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h−1, 1.356% v/v and 1.238% v/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils.

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Section: Discussionsupporting

confidence: 92%

Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica

Roslee

Gómez-Fuentes

Zakaria

et al. 2021

Biology

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“…In the case of other hydrocarbons, the presence of Ni 2+ ions caused: acceleration of the toluene degradation by P1 and P19 strains [33] and inhibition of the crude oil or phenanthrene biodegradation [20,[34][35][36][37]. The bacteria: Pseudomonas aeruginosa CA207Ni, Burkholderia cepacia AL96Co and Corynebacterium kutscheri FL108Hg grew in hydrocarbon media amended with nickel and cobalt (at 5.0 mM) without any changes due to metal toxicity.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nickel as Stress Factor on Phenol Biodegradation by Stenotrophomonas maltophilia KB2

et al. 2021

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This study focuses on the phenol biodegradation kinetics by Stenotrophomonas maltophilia KB2 in a nickel-contaminated medium. Initial tests proved that a nickel concentration of 33.3 mg·L−1 caused a cessation of bacterial growth. The experiments were conducted in a batch bioreactor in several series: without nickel, at constant nickel concentration and at varying metal concentrations (1.67–13.33 g·m−3). For a constant Ni2+ concentration (1.67 or 3.33 g·m−3), a comparable bacterial growth rate was obtained regardless of the initial phenol concentration (50–300 g·m−3). The dependence µ = f (S0) at constant Ni2+ concentration was very well described by the Monod equations. The created varying nickel concentrations experimental database was used to estimate the parameters of selected mathematical models, and the analysis included different methods of determining metal inhibition constant KIM. Each model showed a very good fit with the experimental data (R2 values were higher than 0.9). The best agreement (R2 = 0.995) was achieved using a modified Andrews equation, which considers the metal influence and substrate inhibition. Therefore, kinetic equation parameters were estimated: µmax = 1.584 h−1, KS = 185.367 g·m−3, KIS = 106.137 g·m−3, KIM = 1.249 g·m−3 and n = 1.0706.

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“…(0.211 to 0.335 ppm) and Rhodococcus sp. (0.454 ppm) isolated from the Antarctic in degrading diesel and canola oil, respectively [18,19]. A low IC 50 value means that the metal is more potent at a low concentration and will thus show higher systemic toxicity to the bacteria.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Heavy Metal Tolerance Level of the Antarctic Bacterial Community in Biodegradation of Waste Canola Oil

et al. 2021

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Heavy metal contamination is accidentally becoming prevalent in Antarctica, one of the world’s most pristine regions. Anthropogenic as well as natural causes can result in heavy metal contamination. Each heavy metal has a different toxic effect on various microorganisms and species, which can interfere with other pollutant bioremediation processes. This study focused on the effect of co-contaminant heavy metals on waste canola oil (WCO) biodegradation by the BS14 bacterial community collected from Antarctic soil. The toxicity of different heavy metals in 1 ppm of concentration to the WCO-degrading bacteria was evaluated and further analyzed using half maximal inhibition concentration (IC50) and effective concentration (EC50) tests. The results obtained indicated that Ag and Hg significantly impeded bacterial growth and degradation of WCO, while interestingly, Cr, As, and Pb had the opposite effect. Meanwhile, Cd, Al, Zn, Ni, Co, and Cu only slightly inhibited the bacterial community in WCO biodegradation. The IC50 values of Ag and Hg for WCO degradation were found to be 0.47 and 0.54 ppm, respectively. Meanwhile, Cr, As, and Pb were well-tolerated and induced bacterial growth and WCO degradation, resulting in the EC50 values of 3.00, 23.80, and 28.98 ppm, respectively. The ability of the BS14 community to tolerate heavy metals while biodegrading WCO in low-temperature conditions was successfully confirmed, which is a crucial aspect in biodegrading oil due to the co-contamination of oil and heavy metals that can occur simultaneously, and at the same time it can be applied in heavy metal-contaminated areas.

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Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarctica

Cited by 7 publications

References 37 publications

Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica

Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica

Effect of Nickel as Stress Factor on Phenol Biodegradation by Stenotrophomonas maltophilia KB2

Evaluation of Heavy Metal Tolerance Level of the Antarctic Bacterial Community in Biodegradation of Waste Canola Oil

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