Public forest agencies are obligated to take steps to conserve and where possible enhance biodiversity, but they often lack information and tools that support and evidence their decision making. To help inform and monitor impact of management actions and policies aimed at improving forest biodiversity, we have co-developed a quantitative, transparent and repeatable approach for assessing the biodiversity potential of the United Kingdom's (UK) publicly owned forests over space and time. The FOrest Biodiversity Index (FOBI) integrates several forest biodiversity indicators or ‘metrics’, which characterise management-sensitive woodland and landscape features associated with biodiversity. These are measured or modelled annually using spatially comprehensive forest survey data and other well-maintained spatial environmental datasets. Following metric normalisation and a correlation analysis, a statistically robust selection of these metrics is aggregated using a hierarchical procedure to provide composite index scores. The FOBI metric and index results are provided for every individual public forest, and can be summarised across any reporting region of interest. Compared to existing indicators that rely on sample-based forest data, the results thus better support decisions and obligations at a range of scales, from locally targeted action to national, long-term biodiversity monitoring and reporting. We set out how the FOBI approach and associated bespoke online interfaces were co-developed to meet public forest agency needs in two constituent countries of the UK (England and Scotland), whilst providing a conceptual framework that can be adapted and transferred to other geographic areas and private forests. Example results are reported for England’s public forests for four annual timestamps between 2014 and 2021, which indicate improvements to the biodiversity potential of public forests and surrounding landscapes over this time via increases in their diversity, extent, condition and connectivity.