Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Nagy, Szabolcs; Kakasi, Balázs; Pál, László; Havasi, Máté; Bercsényi, Miklós; Húsvéth, Ferenc

doi:10.1556/018.67.2016.2.1

Cited by 2 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Treatment of cells with Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (Sigma), a respiratory uncoupler/ protonophore, at 50µM [16,17] or 2% paraformaldehyde (Sigma) [18,19] for 30 minutes resulted in significant reduction of MMP (supplementary figure 1C). This certifies the usage of MTDR to investigate MMP, as has been reported in previous studies [20][21][22][23]…”

Section: Immunocytochemical Analyses Of Npcssupporting

confidence: 82%

Studying cellular functions in bipolar disorder: Are there specific predictors of lithium response?

Paul

Iyer

et al. 2019

Preprint

View full text Add to dashboard Cite

BackgroundLithium is the first-line mood stabilizer for the treatment of bipolar disorder (BD). In order to interrogate cellular phenotypes related to disease and lithium treatment response, this study used neural precursor cells (NPCs) and lymphoblastoid cell lines (LCLs) from BD patients who are well characterized for clinical lithium response. MethodsBD patients diagnosed according to the DSM-IV criteria; were recruited from the outpatient services of the National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, India. Clinical lithium response was assessed using the "Alda scale" and "NIMH Retrospective Life chart method". The controls were ethnically matched healthy subjects with no family history of neuropsychiatric illness. NPCs from two BD patients from the same family who clearly differed in their clinical response to lithium were chosen, and compared with healthy population controls. Whole transcriptome sequencing (RNA-Seq) and analysis were performed, with and without in vitro lithium (1mM for 7 days).In addition, mitochondrial membrane potential (MMP), cell viability and cell proliferation parameters were examined. Experiments were also performed in 25 LCLs from BD patients (16 lithium responders and 9 lithium non-responders), and 12 healthy control LCLs, to evaluate them in a system amenable to clinical translation. Results RNA-Sequencing and analysis did not reveal differences inNPCs on in vitro lithium treatment. MMP was lower in BD, both in NPCs and LCLs; reversal with in vitro lithium happened only in LCLs and was unrelated to lithium response. Cell proliferation was higher in BD compared to controls, and there was no change on lithium addition. Cell viability assays indicated greater cell death in BD; which could only be rescued in LCLs of clinical Mitradas M. Panicker (Retd.) Faculty of Neurobiology, NCBS, for providing critical inputs during various parts of the project. We are grateful towards Dr Manjunath, Assistant Professor of Neurovirology, NIMHANS and Phillippo, PhD Scholar, NIMHANS for their constructive criticism and valuable suggestions related to FACS experiment and alsoallowing us to access the Flowcytometry facility. We would also like to thank Ms.Varalakshmi R. and Mr. Suneela Kumar B. for the technical support. We would like to thank the clinicians and staff at the NIMHANS; as well as the subjects and their families for their co-operation.

show abstract

Section: Immunocytochemical Analyses Of Npcssupporting

confidence: 82%

Studying cellular functions in bipolar disorder: Are there specific predictors of lithium response?

Paul

Iyer

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Lithium response in bipolar disorder correlates with improved cell viability of patient derived cell lines

Paul

Iyer

Nadella

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Lithium is an effective, well-established treatment for bipolar disorder (BD). However, the mechanisms of its action, and reasons for variations in clinical response, are unclear. We used neural precursor cells (NPCs) and lymphoblastoid cell lines (LCLs), from BD patients characterized for clinical response to lithium (using the “Alda scale” and “NIMH Retrospective Life chart method”), to interrogate cellular phenotypes related to both disease and clinical lithium response. NPCs from two biologically related BD patients who differed in their clinical response to lithium were compared with healthy controls. RNA-Seq and analysis, mitochondrial membrane potential (MMP), cell viability, and cell proliferation parameters were assessed, with and without in vitro lithium. These parameters were also examined in LCLs from 25 BD patients (16 lithium responders and 9 non-responders), and 12 controls. MMP was lower in both NPCs and LCLs from BD; but it was reversed with in vitro lithium only in LCLs, and this was unrelated to clinical lithium response. The higher cell proliferation observed in BD was unaffected by in vitro lithium. Cell death was greater in BD. However, LCLs from clinical lithium responders could be rescued by addition of in vitro lithium. In vitro lithium also enhanced BCL2 and GSK3B expression in these cells. Our findings indicate cellular phenotypes related to the disease (MMP, cell proliferation) in both NPCs and LCLs; and those related to clinical lithium response (cell viability, BCL2/GSK3B expression) in LCLs.

show abstract

Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Cited by 2 publications

References 20 publications

Studying cellular functions in bipolar disorder: Are there specific predictors of lithium response?

Studying cellular functions in bipolar disorder: Are there specific predictors of lithium response?

Lithium response in bipolar disorder correlates with improved cell viability of patient derived cell lines

Contact Info

Product

Resources

About