Effects of high ELF magnetic fields on enzyme‐catalyzed DNA and RNA synthesis in vitro and on a cell‐free DNA mismatch repair

Harada, Shinichi; Yamada, S.; Kuramata, Osamu; Gunji, Y.; Kawasaki, Masatoshi; Miyakawa, Tomokazu; Yonekura, Hideto; Sakurai, Shigeru; Bessho, K.; Hosono, Ryuji; Yamamoto, Hiroshi

doi:10.1002/bem.48

Cited by 14 publications

(9 citation statements)

References 14 publications

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“…Our attention focused on the enzymatic activity, which is known to be underlying many biological processes. The hypothesis that ELF-MF could affect enzyme activities was tested on a number of soluble enzymes [Litovitz et al, 1991;Zhang and Berg, 1992;Dutta et al, 1994;Thumm et al, 1999;Harada et al, 2001] as well as on membrane-associated enzymes [Blank and Soo, 2001]. Among the latter, two lipidlinked enzymes such as alkaline phosphatase and acetylcholinesterase (AChE) lowered dramatically their activities by about 54% and 58%, respectively [Morelli et al, 2005], when exposed to ELF-MF generated by a square wave of 75 Hz.…”

Section: Introductionmentioning

confidence: 99%

Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes

Ravera¹,

Bianco²,

Cugnoli³

et al. 2009

Bioelectromagnetics

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The effects of extremely low frequency magnetic fields (ELF-MF) on acetylcholinesterase (AChE) activity of synaptosomal membranes were investigated. Sinusoidal fields with 50 Hz frequency and different amplitudes caused AChE activity to decrease about 27% with a threshold of about 0.74 mT. The decrease in enzymatic activity was independent of the time of permanence in the field and was completely reversible. Identical results were obtained with exposure to static MF of the same amplitudes. Moreover, the inhibitory effects on enzymatic activity are spread over frequency windows with different maximal values at 60, 200, 350, and 475 Hz. When synaptosomal membranes were solubilized with Triton, ELF-MF did not affect AChE activity, suggesting the crucial role of the membrane, as well as the lipid linkage of the enzyme, in determining the conditions for inactivation. The results are discussed in order to give an interpretation at molecular level of the macroscopic effects produced by ELF-MF on biological systems, in particular the alterations of embryo development in many organisms due to acetylcholine accumulation.

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Section: Introductionmentioning

confidence: 99%

Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes

Ravera¹,

Bianco²,

Cugnoli³

et al. 2009

Bioelectromagnetics

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“…The assessment of the potential association of magnetic fields and cancer risk is hampered by uncertainties about the effective (magnetic-field) frequency, difficulties of exposure assessment, and the lack of a clear biological mechanism. As possible mechanisms by which low-frequency magnetic fields could be associated with carcinogenesis, their impact on cell proliferation and cell death (17,18), on the occurrence of chromosomal aberrations (19) or DNA damages (18), on the induction or amplification of oxidative stress (20), and on the inhibition of DNA/RNA polymerase (21) were studied.…”

Section: Introductionmentioning

confidence: 99%

The Risk of Lymphoma in AKR/J Mice does not Rise with Chronic Exposure to 50 Hz Magnetic Fields (1 μT and 100 μT)

Sommer

Lerchl

2004

Radiation Research

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Some epidemiological studies suggest that exposure to 50 or 60 Hz magnetic fields might increase the risk of leukemia, especially in children with a comparable high residential exposure. To investigate this possibility experimentally, the influence of 50 Hz magnetic-field exposure on lymphoma induction was determined in a mouse strain that is genetically predisposed to this disease. The AKR/J mouse genome carries the AK virus, which leads within 1 year to spontaneous development of thymic lymphoblastic lymphoma. Beginning at an age of 4-5 weeks, groups of 160 female mice were sham-exposed or exposed to 50 Hz magnetic fields at 1 or 100 microT for 24 h per day, 7 days per week, for 38 weeks. Animals were checked visually daily and were weighed and palpated weekly. There was no effect of magnetic-field exposure on body weight gain or survival rate, and lymphoma incidence did not differ between exposed and sham-exposed animals. Therefore, these data do not support the hypothesis that chronic exposure to 50 Hz magnetic fields is a significant risk factor for developing hematopoietic malignancy.

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“…There were increments in X-ray-induced gene mutations by exposure to AC MFs in NF-kappa B-inhibited cells and in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to electric fields [75]. There was a weak influence of ELF MFs on both in vitro DNA and RNA polymerase activity and cell-free mismatch repair [76]. An effect of UVB radiation and MFs at 100 Hz on DNA synthesis in Jurkat cells was detected [77].…”

Section: Biotargets Of Lower Complexity Exhibited a Relatively Highermentioning

confidence: 97%

A Theoretical Overview of Bioresponse to Magnetic Fields on the Earth’s Surface

Volpe¹

2014

IJG

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This survey points to the mechanisms of bioresponse caused by magnetic fields (MFs), paying attention to their action not only on ions, molecules and macromolecules, but also on cells, tissues and organisms. The significance of findings concerning the MF-dependence of cell proliferation, necrosis or apoptosis was judged by comparing the results obtained in a solenoid, where an MF can be added to the geomagnetic field (GMF), with those obtained in a magnetically shielded room, where the MFs can be attenuated or null. This comparative criterion was particularly appropriate when the differences detectable between the data provided by experimental samples and the data provided by control samples were rather small, as observed in estimating the MF-influence on total DNA replication, RNA transcription and polypeptide translation. The MF-induced inhibition of apoptosis was considered as a risk potentially leading to accumulation of cancer cells. The analysis also surveyed the MF-dependence of the interactions between host animal cells and infecting bacteria. In relation to studies on the origin and adaptation of life on the Earth, theoretical insights paving the way to elucidating the MF-interactions with biostructures and biosystems of different orders of organization evaluated the possible involvement of the so-called "biological windows". Analogously to what is known for ionizing radiations, the efficiency of the applied MFs appeared to depend on the complexity of their biological targets.

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Effects of high ELF magnetic fields on enzyme‐catalyzed DNA and RNA synthesis in vitro and on a cell‐free DNA mismatch repair

Cited by 14 publications

References 14 publications

Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes

Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes

The Risk of Lymphoma in AKR/J Mice does not Rise with Chronic Exposure to 50 Hz Magnetic Fields (1 μT and 100 μT)

A Theoretical Overview of Bioresponse to Magnetic Fields on the Earth’s Surface

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