Aims & Objective: Wearing high heels regularly can lead to biomechanical issues, including tight calf muscles and shorter gastrocnemius muscle fascicles, which compromise musculoskeletal health, affect gait and balance, and heighten the risk of falls. This study compares the effects of two interventions, Instrument-Assisted Soft Tissue Mobilization (IASTM) and Dynamic Cupping Therapy (DCT), on flexibility, balance, and pain reduction in women who regularly wear high heels. Methods: Thirty female participants regularly wearing high heels were randomly assigned to either IASTM or DCT interventions. Outcomes were assessed using measures of flexibility (knee to wall test), balance (App-Coo test), and pain thresholds (algometer). Results: Both IASTM and DCT groups exhibited significant increases in flexion, with IASTM showing a larger mean difference (-3.80, p < 0.01) compared to DCT (-2.73, p < 0.01). Balance with open and closed eyes significantly improved in both groups, with IASTM displaying larger mean differences (0.63, p < 0.01, and 0.57, p = 0.001, respectively) compared to DCT (0.46, p < 0.01, and 0.40, p < 0.01, respectively). Regarding pain reduction, both interventions were effective, but IASTM had a larger mean difference (1.28, p < 0.01) compared to DCT (0.87, p < 0.01). Conclusion: Both IASTM and DCT interventions effectively improved flexibility, balance, and reduced pain in women wearing high heels.