Effects of high intensity interval training and aging on cardiac muscle apoptosis markers in C57BL/6 Mice

Soori, Rahman; Shahneh, Maryam Zare; Choobineh, Siroos; Costa, Pablo B.; Voltarelli, Fabrí­cio Azevedo

doi:10.1007/s11332-020-00670-2

Cited by 6 publications

(13 citation statements)

References 32 publications

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“…In subjects of different ages, regular aerobic exercise has cardioprotective effects and prevents cardiovascular disease (Boeno et al, 2020;Roh et al, 2020). Exercise training by regulating Bax/ Bcl-2 can control apoptosis in mice hearts (Soori et al, 2021). Indeed, we observed that 12 weeks of aerobic exercise training decreased F I G U R E 2 Green tea extract supplementation and exercise-induced changes in the content of Bcl-2 (a, effect of exercise: Yes, effect of green tea: Yes, and effect of both in combination: No), caspase-3 (b, effect of exercise: Yes, effect of green tea: Yes, and effect of both in combination: No), and MDA (c, effect of exercise: Yes, effect of green tea: Yes, and effect of both in combination: No), and GPx activity (d, effect of exercise: Yes, effect of green tea: Yes, and effect of both in combination: No) in rats' heart tissue, after 12 weeks of intervention.…”

Section: Discussionmentioning

confidence: 99%

Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF‐1α/IGFBP3 pathway

Arabzadeh

Kamareh

Ramírez‐Campillo

et al. 2022

Journal of Food Biochemistry

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In this study, we consider the effect of treadmill exercise training, green tea extract, and combination of exercise training with green tea extract, in aging rat cardiac myocytes apoptosis markers (i.e., HIF‐1α, BNIP3, Bax, IGFBP3, Bcl‐2, caspase‐3, MDA, GPx, Bax/Bcl‐2 ratio, and hematoxylin and eosin). Twenty‐four rats (male, Wistar) were divided into four groups: (I) control (n = 6), (II) green tea extract (n = 6), (III) exercise (n = 6), and (IV) exercise + green tea extract (n = 6). Exercise groups performed 12 weeks of running on a rodent treadmill at 17–27 m.min−1 (60–75% vo2peak) for 5 days per week. Green tea extract involved 300 mg.kg−1, 5 days per week for 12 weeks. After being euthanized, the blood and heart were collected for glutathione peroxidase (GPx) activity, malondialdehyde (MDA), HIF‐1α, BNIP3, insulin‐like growth factor‐binding protein‐3 (IGFBP3), Bax, Bcl‐2, caspase‐3, Bax/Bcl‐2 ratio, and hematoxylin and eosin level measurements. Compared to control, the ANOVA demonstrated significant effects of green tea extract (F = 14.646 to 32.453, p = .009 to .001, η = 0.295 to 0.715) and exercise training (F = 9.213 to 133.828, p = .007 to .001, η = 0.315 to η = 0.870) on HIF‐1a, BNIP3, Bax, IGFBP3, Bcl‐2, caspase‐3, MDA, GPx, and Bax/Bcl‐2 ratio. However, the combination of green tea extract and exercise had no effect on the aforementioned apoptosis markers when compared to isolated green tea extract or isolated exercise (F = 0.002 to 4.068, p = .057 to .968, and η = 0.001 to 0.169). Practical applications Isolated exercise training and green tea extract may provide a cardioprotective effect on aging‐induced apoptosis through the downregulation of HIF‐1α, BNIP3, and IGFBP3 in the heart muscle. However, further research is needed to clarify the effects of combining exercise and green tea.

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Section: Discussionmentioning

confidence: 99%

Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF‐1α/IGFBP3 pathway

Arabzadeh

Kamareh

Ramírez‐Campillo

et al. 2022

Journal of Food Biochemistry

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“…However, one should note that the diminished Bcl‐2 expression does not end only with redox failure, while it can trigger the Bax overexpression and/or oligomerization in the outer membrane of mitochondria (Mosadegh et al, 2017; Soori et al, 2021). This situation will be quite particular when it comes to an over‐expression/oligomerization of Bax that in turn can facilitate massive cytochrome c release from mitochondria into the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…The extrinsic apoptosis basically develops through Fas/Fas ligand and TNF‐α/TNFR1 genes interaction (Yasin et al, 2018). Nevertheless, the intrinsic pathway mostly depends on mitochondrial membrane integrity and anti‐ (Bcl‐2 and Bcl‐XL) and pro‐apoptotic (Bax, Bim, and Bad) oncogene expression (Mosadegh et al, 2017; Soori et al, 2021). Consequently, any disruption of Bcl‐2 expression or stability leads to overexpression and oligomerization of Bax, which facilitates the export of Cytochrome C from the mitochondria to the cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

“…Then, Cytochrome c triggers caspase family gene expression (caspases‐3, 6, 7, 8, and 9) in mammalian cells (Mosadegh et al, 2017). The caspase‐3 member of the caspase family is involved in the final stages of apoptosis through the cleavage of DNA and proteins contents of cells (Mosadegh et al, 2017; Soori et al, 2021; Yasin et al, 2018) As a matter of fact, emerging research indicates a cross‐talk between intrinsic (mitochondria‐dependent) apoptosis and oxidative stress (OS); since mitochondria are both sources of reactive oxygen species (ROS) and targets of ROS under OS conditions (Shoorei et al, 2019; Yasin et al, 2018; Zhao et al, 2017). Two major respiratory chain regions of the mitochondrial respiratory system, complex I (NADH coenzyme Q reductase) and complex III (ubiquinol cytochrome c reductase), are known to be major producers of ROS in mammalian cells (Davila et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Running exercise training‐induced impact on oxidative stress and mitochondria‐related apoptosis in rat's testicles

2022

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The current study has been designed to explore the effects of running exercise training protocols (ETPs), with different intensities, on testicular redox and antioxidant capacities. Moreover, the crosstalk between oxidative stress (OS) and mitochondriarelated apoptosis was analysed. To this end, 24 Wistar rats were subdivided into sedentary control, low-(LICT), moderate-(MICT), and high (HICT)-intensity continuous running ETP groups. Following 8 weeks, the Johnsen score, sperm count, testicular malondialdehyde (MDA) content, total oxidant status (TOS), and redox biomarkers, including glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) levels were evaluated. Additionally, the expression levels of Bcl-2, Bax, caspase-3, proteins involving in the mitochondria-related apoptosis, and the apoptotic index were analysed. The LICT and MICT running ETPs did not affect the spermatogenesis development, sperm count, and antioxidant and redox capacities.Accordingly, no significant changes were revealed in Bcl-2, Bax, and caspase-3 expression levels and apoptosis index compared to sedentary rats. In contrast, the HICT-induced rats showed a significant (p < 0.05) reduction in spermatogenesis development, sperm count, antioxidant and redox capacities versus control, LICT, and MICT groups. Moreover, the expression of Bcl-2 was decreased, while the Bax and caspase-3 expression levels were increased in the HICT-induced group. Finally, the apoptosis index was increased in the HICT group. In conclusion, the suppressed redox system after HICT can trigger the mitochondria-mediated ROS overload, result in OS condition in the testicular tissue, and reversely target the mitochondrial membrane permeability. All of these molecular alterations are suspected to initiate progressive mitochondria-related apoptosis after HICT.

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“…Our study results were in line with the findings from Tolouei Azar et al study which reported that 12 weeks of HIIT reduced the number of apoptotic cells in the testicular tissue of high-fat, dietinduced obese rats (19). Soori et al (27) and Pourrazi et al (20) also determined that HIIT training increased the Bcl-2 gene expression but decreased the Bax/Bcl-2 in the cardiac of young and healthy rats. Lee et al suggested that both Fas-dependent (extrinsic pathway) and mitochondrial-dependent apoptotic pathways were more active in obese Zucker (inherited) rats, and three months of exercise training reduced cardiomyocyte apoptosis through both pathways (28).…”

Section: Discussionmentioning

confidence: 96%

The Effect of High Intensity Interval Training on the Intrinsic and Extrinsic Pathways of Myocardial Apoptosis in Diet-Induced Obese Rats

Astani

Bashiri

Pourrazi

et al. 2022

Hormozgan medical journal

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Background: Diet-induced obesity is associated with several cardiovascular diseases and myocardial apoptosis. This study aimed to investigate the effect of high intensity interval training (HIIT) on the myocardial apoptosis in the diet-induced obese rats. Methods: Thirty-two male rats, after an obesity induction period, were randomly selected and assigned to four groups including non-obese control (NOC; n=8), basal obese control (BOC; n=8), obese control (OC; n=8), and obese+HIIT (OT; n=8). NOC and BOC groups were sacrificed before the training period. The OT group underwent a HIIT program performed 5 times/week over 12 weeks. Rat hearts were removed 48 hours after the last training session. The Bax, Bcl-2, cytochrome-c, Bid, caspase-8, as well as caspase-3 protein expressions were analyzed using western blotting. Data were analyzed using independent t test. Results: After obesity induction period, the Bax, Bid, cytochrome-c, caspase-8, caspase-3 proteins, and Bax/Bcl-2 in BOC were found to be significantly higher than those in NOC (P=0.025, P=0.0001, P=0.013, P=0.017, P=0.01, P=0.18, respectively). However, Bcl-2 protein in BOC was detected to be significantly lower than that in NOC (P=0.025). The results obtained after completing HIIT training showed that Bid, cytochrome-c, caspase-8, caspase-3, and Bax/Bcl-2 in OT were lower than those in OC (P=0.005, P=0.039, P=0.001, P=0.04, P=0.05, respectively). However, Bcl-2 protein in OT was significantly higher than that in OC (P=0.004). No significant difference was found between OT and OC regarding Bax protein (P=0.32). Conclusion: Diet-induced obesity may have exacerbated the myocardial apoptosis through both intrinsic and extrinsic pathways. However, it seemed that HIIT training significantly prevented the increase of myocardial apoptosis in obese rats.

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Effects of high intensity interval training and aging on cardiac muscle apoptosis markers in C57BL/6 Mice

Cited by 6 publications

References 32 publications

Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF‐1α/IGFBP3 pathway

Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF‐1α/IGFBP3 pathway

Running exercise training‐induced impact on oxidative stress and mitochondria‐related apoptosis in rat's testicles

The Effect of High Intensity Interval Training on the Intrinsic and Extrinsic Pathways of Myocardial Apoptosis in Diet-Induced Obese Rats

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