Effects of High-Molecular-Weight Sodium Hyaluronate on Experimental Osteoarthrosis Induced by the Resection of Rabbit Anterior Cruciate Ligament

Yoshimi, Tomohisa; Kikuchi, T; Obara, Takeo; Yamaguchi, Toshuiro; Sakakibara, Yasuhiro; Itoh, Hironori; Iwata, Hiroji; Miura, Takayuki

doi:10.1097/00003086-199401000-00039

Cited by 126 publications

(110 citation statements)

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“…12,15 The histologic changes in synovial surface tissue and subsynovial tissue were evaluated and scored. 12,16 Briefly, the grading system assigned separate scores based on two categories: (a) three subcategories of the synovial lining layer: …”

Section: Histologic Assessmentsmentioning

confidence: 99%

Intraarticular gene transfer of thrombospondin‐1 suppresses the disease progression of experimental osteoarthritis

Hsieh

Shen

Shiau

et al. 2010

Journal Orthopaedic Research

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In osteoarthritis, angiogenesis, which occurs in the osteochondral junction and synovium, may accelerate inflammation and contribute to the severity of the disease. We used anterior cruciate ligament-transection (ACLT) to investigate the therapeutic effect of an angiogenesis inhibitor, thrombospondin-1 (TSP-1), in a rat model of osteoarthritis. Osteoarthritis was induced in Wistar rats in the knee of one hind leg. After ACLT, AdTSP-1 (adenoviral vector encoding mouse TSP-1) was intraarticularly injected into the knee joints. Transgene expression, angiogenesis, and inflammatory responses in the knee joints were examined. They were also assessed morphologically, radiographically, and histologically for manifestations of disease. The levels of TSP-1 peaked on day 3 and were substantially maintained for at least 9 days after AdTSP-1 infection. Adenovirus-mediated gene expression was detected in the synovial membrane and chondrocytes. TSP-1 gene transfer induced transforming growth factor-b (TGF-b) production, but it reduced microvessel density, macrophage infiltration, and interleukin-1b (IL-1b) levels. Gross morphological and histopathological examinations revealed that rats treated with AdTSP-1 had less severe osteoarthritis than controls. In vivo adenovirus-mediated TSP-1 gene transfer significantly reduced microvessel density, inflammation, and suppressed the progression of osteoarthritis. This study provides potential applications of TSP-1 gene delivery for treating osteoarthritis. ß

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Section: Histologic Assessmentsmentioning

confidence: 99%

Intraarticular gene transfer of thrombospondin‐1 suppresses the disease progression of experimental osteoarthritis

Hsieh

Shen

Shiau

et al. 2010

Journal Orthopaedic Research

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“…7 A decrease in HA levels in the synovial fluid may result in damage to cartilage surface and increase the permeability of cartilage, allowing proteins and other high molecular weight substances to penetrate into the cartilage matrix. 36 Intraarticularly injected HA binds free proteoglycan in the cartilage matrix, and inhibits further proteoglycan release, thus protecting the cartilage matrix and cells. 7,9,18 HA does not infiltrate normal cartilage.…”

Section: Discussionmentioning

confidence: 99%

“…7,9,18 HA does not infiltrate normal cartilage. 36 Exogenous HA is known to stimulate endogenous HA production by positive feedback. 30 It is speculated that HA may bind inflammatory mediators and free radicals, removing them from the joint space via the lymphatics, thereby reducing cartilage damage.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic acid attenuates osteoarthritis development in the anterior cruciate ligament‐transected knee: Association with excitatory amino acid release in the joint dialysate

Jean

Wen

Chang

et al. 2006

Journal Orthopaedic Research

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We previously reported increased release of the excitatory amino acid (EAA) neurotransmitters, glutamate and aspartate, during the early stage of experimental osteoarthritis (OA). Our present objective was to study the effect of intraarticular injection of hyaluronic acid (HA) on OA development, and to analyze concomitant changes in EAA levels in dialysates of anterior cruciate ligament-transected (ACLT) knee joints. OA was induced in Wistar rats by ACLT of one hindlimb; the knee of the other hindlimb was used as the sham-operated control. HA group (n ¼ 12) were injected intraarticularly in the ACLT knee with 1 mg of HA once a week for 5 consecutive weeks, starting at 8 weeks after surgery. Saline group (n ¼ 12) were injected as above with normal saline. The sham-operated group, underwent arthrotomy, but not ACLT, and received no treatment (n ¼ 14). Twenty weeks after surgery, knee joint dialysates were collected by microdialysis and EAA levels assayed by high-performance liquid chromatography, and gross morphological examination and histopathological evaluation were performed on the medial femoral condyles and synovia. Rats receiving intraarticular HA injections showed a significantly lower degree of cartilage degeneration on the medial femoral condyle at both the macroscopic level and on the Mankin grading scale than rats receiving saline injections. Intraarticular HA treatment also suppressed synovitis. Moreover, glutamate and aspartate levels were significantly reduced in the HA group compared to the saline group. Intraarticular injection of HA limits articular cartilage and synovium damage and OA formation, and, in parallel, reduces EAA levels in ACLT joint dialysates. This study suggests that the underlying mechanism of the anti-inflammatory effect of HA is to inhibit glutamate and aspartate release in ACLT knee joints, which attenuates the early development of OA. ß

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“…The synovium was subjected for cellular assessment by histological scoring system [17] this score assessed (1) synovial lining layer: hyperplasia of synovial lining cells (0-3 points), hypertrophy of synoival lining layer (0-3 points), and infiltration of inflammatory cells (0-3 points); (2) subsynovial tissue: proliferation of granulation tissue (0-3 points), vascularization (0-3 points), and infiltration of inflammatory cells (0-3 points), with a maximum of 18 points. The synvoitis scores were divided into three stages: 0-6 points (mild synovitis), 7-12 points (moderate synovitis), and 13 or more points (severe synovitis).The higher the score, the more the damage.…”

Section: Histological Preparationmentioning

confidence: 99%

Meniscal Degeneration following Anterior Cruciate Ligament Tear and the Role of Intra-Articular Injection of Sodium Hyaluronate. Histochemical and Electron Microscopic Study in the Rabbit Knee

Aly¹

2014

Rheumatology (Sunnyvale)

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Objective: The purpose of this study was to demonstrate the histological changes occurring in the synovium and meniscus after transection of the anterior cruciate ligament in rabbits, and to evaluate these changes after intraarticular injection of sodium hyaluronate.Methods: Fifteen rabbits were divided into three groups. The surgery was performed in the left knees only and the right knees served as controls. Group (I) served as sham-operated controls, Group (II) underwent unilateral anterior cruciate ligament transection of the left knees and received no treatment, and Group (III) received intra-articular injections of 0.3 ml sodium hyaluronate into the left knee beginning 1 week after surgery, once a week for 5 weeks. All rabbits were killed 8 weeks following surgery for assessment of knee meniscus by histological, histochemical and ultrastructural analyses. Results:The histological examination of group II demonstrated the synovium with multilayered synovioblasts, and extensive cellular and matrix deterioration of meniscus in the form of altered cell distribution, decreased cell density, and abnormalities in the collagen arrangement. In groups III, the synovium showed many blood vessels and the cells of menisci apparently increased. Histochemically, safranin-O staining revealed the increased presence of proteoglycan in the sodium hyaluronate treated menisci relative to non-treated one.Ultrastructurally, the chondrocytes of group II showed obvious decrease in their organelles associated with the synthesis and secretions of the matrix with an increase in the number of lysosomes and cytoplasmic vacuoles. In group III, some active chondrocytes containing RER and ribosomes were observed. Conclusions:The results in the present study documented that the treatment with sodium hyaluronate after anterior cruciate ligament transection, induced an improvement of several structural features of both synovial membrane and meniscus.

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Effects of High-Molecular-Weight Sodium Hyaluronate on Experimental Osteoarthrosis Induced by the Resection of Rabbit Anterior Cruciate Ligament

Cited by 126 publications

References 0 publications

Intraarticular gene transfer of thrombospondin‐1 suppresses the disease progression of experimental osteoarthritis

Intraarticular gene transfer of thrombospondin‐1 suppresses the disease progression of experimental osteoarthritis

Hyaluronic acid attenuates osteoarthritis development in the anterior cruciate ligament‐transected knee: Association with excitatory amino acid release in the joint dialysate

Meniscal Degeneration following Anterior Cruciate Ligament Tear and the Role of Intra-Articular Injection of Sodium Hyaluronate. Histochemical and Electron Microscopic Study in the Rabbit Knee

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