Effects of High-Pressure Carbonation on Intracellular ATP and NADH Levels and DNA Damage in Escherichia coli Cells

Abstract

In order to understand the microbial inactivation mechanism of high-pressure carbonation HPC , we examined the changes in the activity of the respiratory chain and DNA damage in Escherichia coli cells. HPC was performed under 1-6 MPa at 30 C for 1 min. The increase in CO 2 pressure decreased the number of viable cells of E. coli, intracellular ATP, and intracellular NADH, and increased the number of apurinic/apyrimidinic sites. These results indicate that HPC has a detrimental effect on the functioning of the respiratory chain in E. coli and induces DNA damage, which could result in the death of the bacterial cells.

Synergetic effect of petit‐high pressure carbon dioxide combined with cinnamon (Cinnamomum cassia) essential oil against Salmonella typhimurium

“…These results were coincident with previous reports that the viable cell population is closely correlated to the intracellular ATP content (Lee et al, 2002). Similar effect of pressurised CO 2 on the intracellular ATP in bacteria cells was also reported by Klangpetch et al (2013). Under pressurisation, CO 2 accumulated within bacteria cells could form carbonic acid and then easily dissociate into bicarbonate (HCO 3 À ), carbonate (CO 2 À ) and hydrogen (H + ) ionic species.…”

“…Similar effect of pressurised CO 2 on the intracellular ATP in bacteria cells was also reported by Klangpetch et al . (2013). Under pressurisation, CO 2 accumulated within bacteria cells could form carbonic acid and then easily dissociate into bicarbonate (HCO 3 − ), carbonate (CO 2 − ) and hydrogen (H + ) ionic species.…”

Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles

“…As the inactivation mechanisms of pressurized CO 2 , leakage of the cytoplasm by damaging the microbial cell membrane and intracellular acidification by the penetration of CO 2 and/or H + have been reported 14–18 . Studies about the inactivation mechanism of CO 2 MB have been performed using Saccharomyces pastorianus cells, and subsequently the following phenomena have been clarified: cell membrane fluidity was reduced by only increasing the temperature above 45°C or by the synergy effect of warming at a temperature below 45°C, CO 2 dissolved in the suspension and intracellular acidification via the penetration of H + , resulting in leakage of the cytoplasm 19,20 ; the synergistic effect of the warming, and H + and CO 2 penetrated in the cells caused the denaturation of intracellular enzymes, suggesting that this is the key factor on the inactivation of S. pastorianus cells 21,22 .…”