Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato

Abstract

Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.

Development of an in vitro Microtuberization and Temporary Immersion Bioreactor System to Evaluate Heat Stress Tolerance in Potatoes (Solanum tuberosum L.)

“…Previous studies on media selection for microtuber induction in vitro in potatoes indicate that higher percentage content of sucrose gave satisfactory results (Hossain et al, 2017 ; Pantelić et al, 2018 ; Guedes et al, 2019 ). Eight percent (8%) sucrose was found to be favorable for microtuber induction.…”

“…High temperature in vitro screening has been achieved for potato clones and True Potato Seed (Khan et al, 2015 ; Guedes et al, 2019 ). The possibility of identifying heat-tolerant potato genotypes through in vitro screening has also been explored by other researchers (Gopal et al, 1998 ; Khan et al, 2015 ; Pantelić et al, 2018 ; Guedes et al, 2019 ). Several microtuberization protocols have been used with solid media or liquid media, with or without hormones, preconditioning with some chemicals or not.…”

Is Cattle Manure Application with Plastic-Film Mulch a Good Choice for Potato Production?

“…Extremely high soil temperature occurred under transparent plastic-film mulch as reported by Zhang et al [29], restraining potato above-ground growth at the seedling stage and tuber initiation and bulking [28]. High temperatures interfered with the onset of micro tuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage [30,31]. Mulching for the whole period of a crop can considerably lower the yield because of consistently higher soil carbon dioxide concentration in the microclimate [32].…”

Antagonism between abscisic acid and gibberellin regulates starch synthesis and corm development in Gladiolus hybridus

“…Tuberization is stimulated under short-day conditions and promoted by low night temperatures 1 . Under long-day or high-temperature conditions, inhibition of tuberization is mediated by the CONSTANTS/FLOWERING LOCUS T (CO/FT) protein 3 , 4 . In hydroponically cultivated potatoes, tuberization is induced by withdrawing nitrogen from the nutrient solution, indicating that nitrogen has an inhibitory effect on tuberization 5 .…”