Effects of high temperature on nodulation and nitrogen fixation by Phaseolus vulgaris L.

Abstract

Screening of Rhizobium leguminosarum bv. phaseoli strains showed some that were able to nodulate common beans (Phaseolus vulgaris L.) at high temperatures (35 and 38°C/8h/day). The nodulation ability was not related to the capability to grow or produce melanin-like pigment in culture media at high temperatures. However, nodules formed at high temperatures were ineffective and plants did not accumulate N in shoots. Two thermal shocks of 40°C/8 h/day at flowering time drastically decreased nitrogenase activity and nodule relative efficiency of plants otherwise grown at 28°C. Recovery of nitrogenase activity began only after seven days, when new nodules formed; total incorporation of N in tops did not recover for 2 weeks. Non-inoculated beans receiving mineral N were not affected by the thermal shock, and when growing continuously at 35 or 38°C had total N accumulated in shoots reduced by only 18%.

Consequences of elevated temperatures on legume biomass and nitrogen cycling in a field warming and biodiversity experiment in a North American prairie

“…Soil temperature affects many aspects of the N 2 fixation process, including nodulation (Barrios et al 1963;Purwantari et al 1995), nodule development (Piha and Munns 1987) and nodule activity (Meyer and Anderson 1959;Hungria and Franco 1993). Both extreme soil temperatures and drought inhibit all of these aspects (Serraj et al 1999;Aranjuelo et al 2007).…”

Characterization of heat resistant mutant strains of Rhizobium sp. [Cajanus] for growth, survival and symbiotic properties

“…These results are in conformity with those obtained by many other workers. It has been reported that with constant high temperature (35 o C or more) treatment, either there is no nodulation 19 or the nodules are ineffective 20 . But, similar to our results, the diurnal cyclic temperature regimes like 41 o C for 6 or 9 h day -1 have shown to cause nodulation on the host plants by all the strains of bradyrhizobia 21 .…”

Infestação por larvas de Cerotoma arcuata (Olivier) (Coleoptera: Chrysomelidae) em nódulos de feijoeiro em cultivo com cobertura morta ou em consórcio com milho ou com caupi

“…Rhizobium sp. é afetado em temperaturas acima de 35 o C (HUNGRIA & FRANCO, 1993). Entretanto, durante o experimento, houve uma variação de temperatura entre 20,2 e 25,5 o C. Uma vez que o experimento foi irrigado sempre que necessário e o feijoeiro foi inoculado com estirpes de rizóbio tolerantes a altas temperaturas, é provável que os benefícios da cobertura morta na produção de grãos tenham decorrido, em grande parte, da proteção contra as larvas de C. arcuata.…”