Effects of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish ( Takifugu obscurus )

Cheng, Chang-Hong; Guo, Zhiyong; Luo, Sheng‐Wei; Wang, An‐Li

doi:10.1016/j.ecoenv.2017.12.045

Cited by 127 publications

(43 citation statements)

References 57 publications

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“…As important markers for physiology, pathology, and toxicology research, the blood physiological and biochemical parameters of rhesus macaques have not been characterized comprehensively. These markers directly reflect physical health status and are useful for clinical diagnosis [18, 19]. Although the hematological and biochemical parameters of Macaca fascicularis [20, 21], Sulawesi macaques [22], and Tonkean macaques [23] have been reported, those of Chinese rhesus macaques have been rarely investigated.…”

Section: Introductionmentioning

confidence: 99%

Hematological and biochemical parameters for Chinese rhesus macaque

Hao

Yang

et al. 2019

PLoS ONE

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Rhesus macaque is an important animal model in biomedical research, especially human disease, developmental, translational, and pre-clinical research. Blood physiological and biochemical parameters are important markers for physiology, pathology, and toxicology research. However, these parameters have not been systematically reported for Chinese rhesus macaques. To characterize the reference for these parameters, this study collected 1805 Chinese rhesus macaques living in Southwestern China. A total of 24 blood physiological indexes and 27 biochemical parameters were determined. Sex and age were found to affect these parameters. In conclusion, a comprehensive and systematic reference of hematological and biochemical parameters for Chinese rhesus macaque was established in this work on the basis of a large cohort. Such reference will benefit biomedical research employing rhesus macaques as animal models.

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Section: Introductionmentioning

confidence: 99%

Hematological and biochemical parameters for Chinese rhesus macaque

Hao

Yang

et al. 2019

PLoS ONE

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“…Oxidative stress occurs when redox homeostasis is disrupted, and the cellular ROS overproduction results from the imbalance between oxidants and antioxidants [29]. SOD, GSH-Px, and CAT are key antioxidative enzymes in the defense against oxidative stress, and they act by scavenging ROS [30]. SOD and GSH-Px play an essential role in scavenging free radicals, and CAT can catalyze the decomposition of H 2 O 2 to produce molecular oxygen (O 2 ) and H 2 O [31].…”

Section: Discussionmentioning

confidence: 99%

Astragaloside IV Protects Against Oxidative Stress in Calf Small Intestine Epithelial Cells via NFE2L2-Antioxidant Response Element Signaling

Wang

Jiang

Cheng

et al. 2019

IJMS

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Oxidative stress can damage intestinal epithelial cell integrity and function, causing gastrointestinal disorders. Astragaloside IV (ASIV) exhibits a variety of biological and pharmacological properties, including anti-inflammatory and antioxidant effects. The purpose of this research was to investigate the cytoprotective action of ASIV and its mechanisms in calf small intestine epithelial cells with hydrogen peroxide (H2O2)-induced oxidative stress. ASIV pretreatment not only increased cell survival, but it also decreased reactive oxygen species generation and apoptosis, enhanced superoxide dismutase, catalase, and glutathione peroxidase levels, and it reduced malondialdehyde formation. Furthermore, pretreatment with ASIV elevated the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (NFE2L2), heme oxygenase-1 (HMOX1), and NAD(P)H quinone dehydrogenase 1 (NQO1). The NFE2L2 inhibitor ML385 inhibited NFE2L2 expression and then blocked HMOX1 and NQO1 expression. These results demonstrate that ASIV treatment effectively protects against H2O2-induced oxidative damage in calf small intestine epithelial cells through the activation of the NFE2L2-antioxidant response element signaling pathway.

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“…The activities of AST, ALT, and alkaline phosphatase (ALP) were used as indicators of liver damage in fish [19]. The emission of ALT and AST may be caused by liver damage under high temperatures and they increased in Temp group.…”

Section: Discussionmentioning

confidence: 99%

“…In general, the monitoring of hematological parameters such as plasma cortisol and metabolic enzyme activities are used to detect stress in fish [18][19][20]. However, these detectable physiological responses cannot always be elicited as stress responses, and the lack of these factors does not always mean that the fish are stressed.…”

Section: Introductionmentioning

confidence: 99%

Glutathione Injection Alleviates the Fluctuation of Metabolic Response under Thermal Stress in Olive Flounder, Paralichthys olivaceus

Kim

et al. 2019

Metabolites

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Continuous increases in water temperature disturb homeostasis and increase oxidative stress in fish. Glutathione (GSH) is an intracellular antioxidant that helps to relieve stress in animals. In this study, we observed the effect of GSH on olive flounder exposed to high temperature using serum parameters and NMR-based metabolomics. Based on the results from the first experiment, 20 mg of GSH was chosen as an effective dose with lower infection rates and mortality. Then, fish were divided into Control, Temp (PS injection), and GSH (glutathione injection) groups, and fish in Temp and GSH groups were exposed to temperature fluctuations (20 °C→24 °C→27 °C). In OPLS-DA score plots, Temp group was clearly distinguished from the other groups in the kidney. In the liver, the metabolic patterns of GSH group were close to the Temp group on day 4 and became similar to Control group from day 7. Serum parameters did not change significantly, but the deviation in Temp group was greater than that in GSH group. Metabolite levels that were significantly altered included GSH, lactate, O-phosphocholine, and betaine in the kidney and taurine, glucose, and several amino acids in the liver, which were related to antioxidant activity and energy system. Therefore, GSH supplements could relieve thermal stress influencing metabolic mechanisms in fish.

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Effects of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish ( Takifugu obscurus )

Cited by 127 publications

References 57 publications

Hematological and biochemical parameters for Chinese rhesus macaque

Hematological and biochemical parameters for Chinese rhesus macaque

Astragaloside IV Protects Against Oxidative Stress in Calf Small Intestine Epithelial Cells via NFE2L2-Antioxidant Response Element Signaling

Glutathione Injection Alleviates the Fluctuation of Metabolic Response under Thermal Stress in Olive Flounder, Paralichthys olivaceus

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