Effects of histone deacetylase inhibitors on regenerative cell responses in human dental pulp cells

Luo, Zheng; Wang, Z.; He, Xinyao; Liu, N.; Liu, B.; Sun, Liguo; Wang, J.; Duncan, Henry F.; Wang, He; Cooper, Paul R.

doi:10.1111/iej.12779

Cited by 16 publications

(18 citation statements)

References 46 publications

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“…The proliferation of DPSCs is increased on exposure to 2 nmol/L and 20 nmol/L of TSA via the activation of the JNK/c-Jun pathway; however, higher concentrations of TSA lead to apoptosis. A 20 nmol/L solution of TSA can promote migration and adhesion of DPSCs [ 137 – 140 ]. Valproic acid (VPA), the short-chain fatty acid, can inhibit class I HDACs.…”

Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

“…Valproic acid (VPA), the short-chain fatty acid, can inhibit class I HDACs. Similar to TSA, the effect of VPA on DPSCs is dose-dependent, and at a certain concentration, it can improve the proliferation, adhesion, and migration of DPSCs [ 137 ]. In addition, VPA increases the mineralization and osteo/odontogenic differentiation [ 138 , 139 ].…”

Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

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Epigenetic Regulation of Dental Pulp Stem Cell Fate

Zhou

Gan

Yan

et al. 2020

Stem Cells International

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Epigenetic regulation, mainly involving DNA methylation, histone modification, and noncoding RNAs, affects gene expression without modifying the primary DNA sequence and modulates cell fate. Mesenchymal stem cells derived from dental pulp, also called dental pulp stem cells (DPSCs), exhibit multipotent differentiation capacity and can promote various biological processes, including odontogenesis, osteogenesis, angiogenesis, myogenesis, and chondrogenesis. Over the past decades, increased attention has been attracted by the use of DPSCs in the field of regenerative medicine. According to a series of studies, epigenetic regulation is essential for DPSCs to differentiate into specialized cells. In this review, we summarize the mechanisms involved in the epigenetic regulation of the fate of DPSCs.

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Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Zhou

Gan

Yan

et al. 2020

Stem Cells International

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“…HDACis reduced cell proliferation and viability at relatively high doses, but at lower doses did not show cytotoxic or anti-proliferative effects (Duncan et al, 2013;Paino et al, 2014;Duncan et al, 2017). SAHA was also shown to promote other reparative processes in DPC populations, including cell migration (Duncan et al, 2016;Luo et al, 2018) and cell adhesion (Luo et al, 2018). In addition to the direct regulation of SCs, HDACis also induce bioactive DMC release from dentine (Duncan et al, 2017).…”

Section: Hdaci In Regenerative Endodontic Therapiesmentioning

confidence: 99%

“…Several HDAC inhibitors (HDACis), including trichostatin A (TSA), valproic acid (VPA), and suberoylanilide hydroxamic acid (SAHA), have been shown to have clinical application in a range of diseases including cancer and inflammatory and neurodegenerative disorders (Bolden et al, 2006;Das Gupta et al, 2016;Naftelberg et al, 2017). The medical and dental literature also reports that HDACis are associated with anti-inflammatory effects, pro-mineralisation, increased SC differentiation, and overall improved regenerative responses (Halili et al, 2009;Xu et al, 2009;Wang et al, 2010;Duncan et al, 2013;Luo et al, 2018). Consequently, HDACis have the potential to enhance dentine regenerative processes in VPT by directly influencing DSC populations (Duncan et al, 2012;Luo et al, 2018) and indirectly, by inducing the solubilisation of dentine matrix components (DMCs) rich in growth factors (GFs) and other bioactive molecules (Smith et al, 2016;Duncan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The medical and dental literature also reports that HDACis are associated with anti-inflammatory effects, pro-mineralisation, increased SC differentiation, and overall improved regenerative responses (Halili et al, 2009;Xu et al, 2009;Wang et al, 2010;Duncan et al, 2013;Luo et al, 2018). Consequently, HDACis have the potential to enhance dentine regenerative processes in VPT by directly influencing DSC populations (Duncan et al, 2012;Luo et al, 2018) and indirectly, by inducing the solubilisation of dentine matrix components (DMCs) rich in growth factors (GFs) and other bioactive molecules (Smith et al, 2016;Duncan et al, 2017). An emerging role for HDACs in tooth development and regeneration presents an opportunity for HDACi use in novel dental regenerative materials.…”

Section: Introductionmentioning

confidence: 99%

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Histone Acetylation as a Regenerative Target in the Dentine-Pulp Complex

et al. 2020

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MicroRNA expression profiles of neural stem cells following valproate inducement

Peng

et al. 2018

J of Cellular Biochemistry

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Neural stem cells (NSCs) possess self-renewal and multilineage differentiation ability, thus are considered to be a potential source for cell replacement therapy of many nervous system diseases, such as neurodegenerative diseases. Valproate (VPA), a member of histone deacetylase inhibitor family, is an epigenetic regulator and can promote NSCs to differentiate into neurons, nevertheless, the underlying mechanisms of the process remain unclear. MicroRNAs (miRNAs) exert a crucial part in the posttranscriptional regulation of gene expression. Epigenetic mechanisms involve in the regulation of miRNAs expression. Therefore we speculated that miRNAs may be important factors during the promotion of neuronal differentiation by VPA. Here, after selecting appropriate concentration and treatment time of VPA, we conducted microRNA arrays at 24 h on the treatment of 1 mM VPA or vehicle. After validation, we obtained 5 significantly upregulated miRNAs (miR-29a-5p, miR-674-5p, miR-155-5p, miR-652-3p, and miR-210-3p) in VPA group compared with control. We predicted the target genes of these miRNAs on the website. Through gene ontology (GO) and pathway analyses, we obtained preliminary comprehension of the function of these genes. The bioinformatics analyses indicated the involvement of them during neurogenesis. In addition, we observed high expression of miR-210-3p, miR-29a-5p, and miR-674-5p in central nervous system, which suggested that they were likely to play crucial roles in neuronal differentiation. We then defined the upregulation of Map2 by transfecting mimic of miR-674-5p, which indicated the promotion of miR-674-5p on NSCs differentiation. The present study explored the miRNAs potentially mediated the function of VPA on promoting NSCs to differentiate into neurons.

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Effects of histone deacetylase inhibitors on regenerative cell responses in human dental pulp cells

Abstract: Histone deacetylase inhibitors at specific concentrations promoted proliferation, migration and adhesion of hDPSCs, which may contribute to novel regenerative therapies for pulpal disease treatment.

Cited by 16 publications

References 46 publications

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Histone Acetylation as a Regenerative Target in the Dentine-Pulp Complex

MicroRNA expression profiles of neural stem cells following valproate inducement

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