Effects of homobrassinolide in barley callus culture

Temel, Aslıhan; Gözükırmızı, Nermin

doi:10.17221/305/2012-pse

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Barley ( Hordeum vulgare cv. Tokak) seeds were provided by the Aegean Agricultural Research Institute and cultured as described previously ( Temel and Gozukirmizi, 2012 ). Embryos were cultured in MS (Murashige and Skoog) medium supplemented with 0 (Control), 50 and 100 mM NaCl, in a growth chamber (Sanyo).…”

Section: Methodsmentioning

confidence: 99%

Genetic and epigenetic effects of salinity on in vitro growth of barley

et al. 2013

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Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.

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Section: Methodsmentioning

confidence: 99%

Genetic and epigenetic effects of salinity on in vitro growth of barley

et al. 2013

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Diversity of individuals' methylation patterns to different moisture regimes in Einkorn wheat revealed by CRED-RA technique

Hosseini,

Zarei,

Cheghamirza

2023

Genet Resour Crop Evol

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Among the wild relatives of wheat, diploid species with desirable characteristics have high potential in crop modi cation. One of the important epigenetic changes that is associated with the pattern of gene expression and causes genetic instability is DNA methylation. The coupled restriction enzyme digestionrandom ampli cation (CRED-RA) technique and ve RAPD primers were used to study methylation changes in two Einkorn wheat genotypes including S10 and H9 genotypes and four soil water availability treatments included control, mild, moderate and severe stress (irrigation at 100%, 75%, 50% and 25% of eld capacity, respectively) in two stages (14 days after water de ciency and 14 days after re-watering). The experiment was laid out as 2×4 factorial experiment within completely randomized design replicated three times. Measured physiological traits included relative leaf water content, Fv / Fm and stomatal conductance. After 14 days of exposure to drought stress, the results showed that the lowest relative water content of leaves, Fv/Fm and stomatal conductance were related to severe stress treatment. Also, S10 genotype was superior to H9 after application of moisture stress treatments and after re-watering. Different patterns of methylation were observed in different replications of each treatments in response to severe water de ciency, which can be due to the diversity of individuals' response to stress and the speci city of epigenetics changes. Most of the fragments that showed a change in methylation pattern due to water de ciency, after stress removal, returned to the state before stress, which indicates the reversibility of methylation changes in the genome.

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