Effects of hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat cells (1982) FEBS Letters 146, 327–330.

Brooks, Brian J.; Arch, Jrs; Newsholme, Eric A.

doi:10.1016/0014-5793(82)81291-x

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“…It is unlikely that PUFA enrichment of the effluent was the result of phospholipase C activation by al-receptor activity. Brooks et al (29) Faily acid enrichment Figure 5 shows the proportions of radiolabeled oleate and a-linolenate released from the TG analog substrata The molar ratio a-linolenate/oleate in the substrate was 1.06 • 0.01 (n ---4). The enzyme reaction hydrolyzed 3-6% of the total esterified fatty acid during the 30-min incubation period.…”

Section: Resultsmentioning

confidence: 99%

Adipose hormone‐sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides

Gavino

1992

Lipids

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Rat adipose hormone-sensitive lipase-mediated release of fatty acids from triglycerides was studied in three model systems: i) cultured preadipocytes containing polyunsaturated fatty acid-enriched triglyceride; ii) perfused epididymal fat pads; and iii) in vitro incubations of crude preparations of hormone-sensitive lipase with synthetic triglyceride-analogues as substrates. We found that cultured preadipocytes challenged with 10 microM norepinephrine tended to release more omega 6 and omega 3 polyunsaturated fatty acids than saturated fatty acids. Fat pads perfused with 10 microM norepinephrine preferentially released arachidonate and alpha-linolenate but tended to retain oleate and linoleate. Finally, crude preparations of hormone-sensitive lipase released from the triglyceride-analogue substrates alpha-linolenate twice as fast as oleate. We conclude that rat adipose hormone-sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides. We suggest that this may be a mechanism by which these fatty acids are kept from being trapped in fat depots and maintained in the circulation.

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Section: Resultsmentioning

confidence: 99%

Adipose hormone‐sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides

Gavino

1992

Lipids

View full text Add to dashboard Cite

show abstract

Effects of hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat cells (1982) FEBS Letters 146, 327–330.

Cited by 1 publication

References 0 publications

Adipose hormone‐sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides

Adipose hormone‐sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides

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