Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of Spodoptera frugiperda

Chen, Yaping; Li, Yahong; Sun, Zhongxiang; Du, Ewei; Lu, Zhihui; Li, Hao; Gui, Furong

doi:10.3390/insects13040373

Cited by 11 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The influences of different genotypes of maize ( Zea mays ), including B73, Tx601, and Mp708, on S. frugiperda larval midgut community structure and composition were also explored 22 . Significant differences in gut microbial community structure and diversity were exhibited when the larvae fed on different hosts 23 , 24 . These studies could contribute to the research of the host adaptation of S. frugiperda and the development of efficient and environmentally friendly control strategies.…”

Section: Introductionmentioning

confidence: 99%

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

Yuning

Luyang

Xueming

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Spodoptera frugiperda is a highly polyphagous pest worldwide with a wide host range that causes serious losses to many economically important crops. Recently, insect-microbe associations have become a hot spot in current entomology research, and the midgut microbiome of S. frugiperda has been investigated, while the effects of cruciferous vegetables remain unknown. In this study, the growth of S. frugiperda larvae fed on an artificial diet, Brassica campestris and Brassica oleracea for 7 days was analyzed. Besides, the microbial community and functional prediction analyses of the larval midguts of S. frugiperda fed with different diets were performed by high-throughput sequencing. Our results showed that B. oleracea inhibited the growth of S. frugiperda larvae. The larval midgut microbial community composition and structure were significantly affected by different diets. Linear discriminant analysis effect size (LEfSe) suggested 20 bacterial genera and 2 fungal genera contributed to different gut microbial community structures. The functional classification of the midgut microbiome analyzed by PICRUSt and FUNGuild showed that the most COG function categories of midgut bacterial function were changed by B. oleracea, while the guilds of fungal function were altered by B. campestris significantly. These results showed that the diversity and structure of the S. frugiperda midgut microbial community were affected by cruciferous vegetable feeding. Our study provided a preliminary understanding of the role of midgut microbes in S. frugiperda larvae in response to cruciferous vegetables.

show abstract

Section: Introductionmentioning

confidence: 99%

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

Yuning

Luyang

Xueming

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In recent years, there has been an increasing number of studies on the gut microbial diversity of S. frugiperda from different environments or fed on different diets ( Gomes et al, 2020 ; Rozadilla et al, 2020 ; Ugwu et al, 2020 ; Chen et al, 2021 , 2022 ; Higuita Palacio et al, 2021 ; Lv et al, 2021 ; Ye et al, 2021 ). However, these studies mainly focused on the gut microbial community associated with a certain stage of larvae in S. frugiperda , providing only a single snapshot of the bacterial community.…”

Section: Introductionmentioning

confidence: 99%

Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction

Fu,

Wang,

Huang

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionSpodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.MethodsHere, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction.ResultsProteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction.DiscussionOur findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.

show abstract

“…Shatangju [ 7 ]. Host plants have a significant impact on the structure and composition of gut bacteria in Spodoptera frugiperda [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Source and Transmission Pathway of Gut Bacteria in the Diamondback Moth, Plutella xylostella

Han

2023

Insects

View full text Add to dashboard Cite

Plutella xylostella (L.), commonly known as the diamondback moth, is currently a major worldwide pest. Gut bacteria play an important role in the physiology and insecticide resistance of P. xylostella, but little is known about the sources and transmission routes of its gut bacteria. In this study, traditional microbial culture methods were used to analyze the sources and transmission modes of gut bacteria in P. xylostella, which could help develop pest control strategies based on gut bacteria. The main findings are as follows: gut bacterial diversity was significantly higher in P. xylostella-fed radish sprouts than those fed an artificial diet, indicating a potential association between gut bacteria and food bacteria. In addition, sequence analysis revealed the isolation of Enterobacter sp., Pantoea sp., Cedecea sp., and Pseudomonas sp. from both radish sprouts and P. xylostella. Importantly, Enterobacter sp. was found in all tested samples (radish sprouts, gut of P. xylostella, ovaries, and eggs), suggesting that bacteria acquired from food could be transferred from the gut to the ovaries and eggs. This was confirmed through experiments, which also showed that eggs could carry bacteria and transmit them to the gut, indicating vertical transmission of gut bacteria via eggs. Furthermore, the 3rd instar larvae of P. xylostella with and without gut bacteria were mixed and raised until the 4th instar. Then, we found that all the gut of the 4th instar larvae carried the same bacteria, indicating that the gut bacteria of P. xylostella can be horizontally transmitted through social behavior. This study lays a foundation for further exploration of the sources, transmission, and coevolution of the host of gut bacteria in P. xylostella, and provides new insights into pest control strategies based on the source and transmission of gut bacteria.

show abstract

Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of Spodoptera frugiperda

Cited by 11 publications

References 49 publications

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables

Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction

Potential Source and Transmission Pathway of Gut Bacteria in the Diamondback Moth, Plutella xylostella

Contact Info

Product

Resources

About