Effects of HSPA8, an evolutionarily conserved oviductal protein, on boar and bull spermatozoa

Elliott, R. M.; Lloyd, Rhiannon; Fazeli, Alireza; Šoštarić, Edita; Georgiou, A. S.; Satake, Nana; Watson, P. F.; Holt, William V.

doi:10.1530/rep-08-0298

Cited by 98 publications

(96 citation statements)

References 38 publications

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“…One member of this family, HSPA8, secreted by the oviduct, was shown to support the viability of spermatozoa in the boar, the ram, and the bull (46,51). The increase of abundance of HSPA8 we found in the oviduct fluid at estrus may be linked to a role in sperm survival around ovulation.…”

Section: Discussionmentioning

confidence: 64%

Proteomes of the Female Genital Tract During the Oestrous Cycle

Soleilhavoup

Riou

Tsikis

et al. 2016

Molecular & Cellular Proteomics

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The success of fertilisation in mammals is linked to the correct migration of spermatozoa in the different compartments of the female genital tract and the adequate timing of their interaction with the female gamete. In many mammalian species including human, the deposition of semen in the vagina is followed by the sperm migration through the cervix, the uterus and then the oviduct before reaching the site of fertilisation. This sperm transit within the female tract includes mechanical and biochemical interactions with the luminal fluids leading to selection of spermatozoa able to fertilize the oocyte.The first physiological barrier the spermatozoa will have to go through is the cervix whose vaginal side is covered by a highly viscous mucus, the cervical vaginal fluid (CVF) 1 . The CVF proteome was analyzed in humans in various physiological conditions (1, 2), mainly during pregnancy (3-7) or in several pathologies such as human papilloma virus (8) or candida albicans (9) infections. But few studies have investigated the CVF proteome during the cycle (10, 11). The amount of CVF increases at the time of ovulation concomitantly with a higher state of hydration and a reduced viscosity, to facilitate sperm migration (12). The mechanical properties of the mucus are essential to select spermatozoa with the highest fertilizing ability, i.e. with normal morphology and efficient mobility. The main structural components of the cervical mucus are mucins, highly glycosylated, high-molecular-weight proteins assembled into a filamentous and viscous mesh (13). The transcription of the mucin genes is increased at oestrus in the bovine cervical epithelium (14). The amount of mucins and their level of glycosylation are expected to contribute to the viscosity of the mucus (11). Therefore, the quantification of proteins such From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements,

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Section: Discussionmentioning

confidence: 64%

Proteomes of the Female Genital Tract During the Oestrous Cycle

Soleilhavoup

Riou

Tsikis

et al. 2016

Molecular & Cellular Proteomics

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“…Previous studies have shown that HSPA8 exerts protective effects on spermatozoa, enhancing sperm survival at body temperature in vitro in boars, rams, and bulls [7][8][9][10]. The objective of the present study was to extend these studies and investigate the possibility that the protective effects of HSPA8 might be exploited for the improvement of bovine sperm cryopreservation protocols.…”

Section: Discussionmentioning

confidence: 95%

“…Although it often functions at the intracellular level, it has also been detected in the extracellular space, where it exerts protective effects on cell viability, apparently possessing both proapoptotic and antiapoptotic properties depending on the situation [3,4]. Within the context of sperm biology, recent studies have shown that HSPA8 is produced by oviductal epithelial cells during sperm transport [5,6] and enhances boar, ram, and bull sperm survival [7][8][9][10] in vitro.…”

Section: Introductionmentioning

confidence: 99%

Heat shock protein A8 stabilizes the bull sperm plasma membrane during cryopreservation: Effects of breed, protein concentration, and mode of use

Holt¹,

Valle²,

Fazeli³

2015

Theriogenology

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“…These are potentially important findings, as the oviduct and oviductal sperm storage play key roles in reproduction by providing a secure reservoir in which spermatozoa can attain full fertilising properties. Hsc70 appears to interact with components of the sperm cell surface membrane (117,118) and exposure to Hsc70 prolongs the survival of boar, bull and ram sperm (117,120). Studies have now shown that a recombinant form of Hsc70 (HSPA8) rapidly promotes the viability of uncapacitated spermatozoa, the ability of spermatozoa to bind to oviductal epithelial cells, enhances the performance of in vitro fertilisation procedures, and decreases sperm mitochondrial activity (121).…”

Section: Extracellular Cell Stress Proteins In Cancermentioning

confidence: 99%

Extracellular cell stress (heat shock) proteins—immune responses and disease: an overview

Pockley

Henderson

2017

Phil. Trans. R. Soc. B

117

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Contribution to a theme issue 'Heat shock proteins in health and disease: integrating knowledge to better understand the role of heat shock proteins as therapeutic targets and disease modulators' Keywords:Heat shock (stress) proteins, extracellular, immunity Author for correspondence:A. Graham Pockley, PhD e-mail: graham.pockley@ntu.ac.ukHeat shock proteins in health and disease: integrating knowledge to better understand the role of heat shock proteins as therapeutic targets and disease modulators 2 | P a g e Abstract Extracellular cell stress proteins are highly conserved phylogenetically and have been shown to act as powerful signalling agonists and receptors for selected ligands in several different settings. They also act as immunostimulatory "danger signals" for the innate and adaptive immune systems. Other studies have shown that cell stress proteins and the induction of immune reactivity to self-cell stress proteins can attenuate disease processes. Some proteins (e.g. Hsp60, Hsp70, gp96) exhibit both inflammatory and anti-inflammatory properties, depending on the context in which they encounter responding immune cells.The burgeoning literature reporting the presence of stress proteins in a range of biological fluids in healthy individuals / non-diseased settings, the association of extracellular stress protein levels with a plethora of clinical and pathological conditions and the selective expression of a membrane form of Hsp70 on cancer cells now supports the concept that extracellular cell stress proteins are involved in maintaining / regulating organismal homeostasis and in disease processes and phenotype. Cell stress proteins therefore form a biologically complex extracellular cell stress protein network having diverse biological, homeostatic and immunomodulatory properties, the understanding of which offers exciting opportunities for delivering novel approaches to predict, identify, diagnose, manage and treat disease.Heat shock proteins in health and disease: integrating knowledge to better understand the role of heat shock proteins as therapeutic targets and disease modulators 3 | P a g e 1. Background 'Chance favours the prepared mind ' -Louis Pasteur (1822 -1895 The presence of additional new 'puffs' in the polytene chromosomes of cultured Drosophila larva which were induced following their incubation at an inadvertently high temperature and observed by Ferruccio Ritossa (25 th February 1936 -9 th January 2014) in the early 1960s was unexpected and puzzling. He realised the potential importance of this first evidence that stress can influence gene transcription and induce the synthesis of new proteins, yet found it surprisingly difficult to publish this discovery. It was eventually published in Experientia (1, 2).Ritossa's findings were extended and expanded upon during the next decade and by the mid-to-late 1960s it was clear that exposure of cells containing polytene chromosomes to a variety of environmental stressors resulted in the transcription of novel genes and, presumably, in the synthesi...

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Effects of HSPA8, an evolutionarily conserved oviductal protein, on boar and bull spermatozoa

Cited by 98 publications

References 38 publications

Proteomes of the Female Genital Tract During the Oestrous Cycle

Proteomes of the Female Genital Tract During the Oestrous Cycle

Heat shock protein A8 stabilizes the bull sperm plasma membrane during cryopreservation: Effects of breed, protein concentration, and mode of use

Extracellular cell stress (heat shock) proteins—immune responses and disease: an overview

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