Effects of Human Growth Hormone on Haematopoietic Recovery of Rats Receiving Chemotherapy

Abstract

Human growth hormone (hGH) is a pleiotropic cytokine targeting a variety of tissues. Its protective effects on haematopoiesis during treatment with chemotherapeutic drugs were investigated. Sprague-Dawley rats were intraperitoneally administered with both carboplatin and 5-fluorouracil together with or without recombinant hGH (rhGH) at a dose of 1 IU/kg/day. Body weight, full blood count, bone marrow differential count and expression of proliferating cell nuclear antigen (PCNA) in bone marrow were measured weekly for 4 weeks in chemotherapy-treated (CT) or chemotherapy plus rhGH-treated (CT+GH) animals. During the first week, body weight, white blood cell count, haematopoietic count and reticulocyte count were decreased in the CT group as well as in the CT+GH group, but to a lesser extent in the latter group (CT vs. CT+GH group, p < 0.05). Further decreases were also prevented in the CT+GH group. Myeloid cells were extremely hypoplastic in the CT group, while in the CT+GH group, myeloid cells recovered from obviously hypoplastic to excessively hyperplastic in the first 2 weeks, and the myeloid karyocyte count increased significantly (CT+GH vs. CT group, p < 0.05). PCNA-positive cell count in the CT+GH group was also significantly higher than in the CT group (CT+GH vs. CT group, p < 0.05). Thus, rhGH showed a promising protective effect on body weight loss and haematopoietic recovery of chemotherapy-treated rats, which may be useful in clinic to reduce the side effects of chemotherapy.

hGH promotes megakaryocyte differentiation and exerts a complementary effect with c-Mpl ligands on thrombopoiesis

“…2 Thereafter, increasing studies reported that a high dose of GH could restore hematopoietic functions, including platelet recovery in mice and rats, after radiation, chemotherapy, or bone marrow (BM) transplantation. [3][4][5][6][7] An in vitro study further revealed that human growth hormone (hGH) has a distinct ability to stimulate the proliferation and differentiation of multilineage hematopoietic cells. 8 Later, a clinical investigation showed that hematologic patients with recombinant human growth hormone (rhGH) treatment underwent a faster recovery of platelets to 25 3 10 9 /L after intensive chemotherapy.…”

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency

“…As a member of the growth factor family, it plays a vital role in regulating growth and development, the gonadal axis, metabolism, and the psychological status (Ben-Avraham et al, 2017;Nylander et al, 2018). Recently, it has been identified to be involved in attenuating the injury caused by chemotherapy via promoting hematopoietic recovery (Zhang et al, 2008) and repairing the intestinal mucosal barrier (Ortega et al, 2001). More importantly, it has also been shown to be associated with fertility and fecundity (Satoh et al, 2002).…”

Development of a sandwich enzyme-linked immunosorbent assay for dTMP-GH fusion protein by rational immunogen selection

“…platelet) in DW/J dwarf mice deficient in GH (Murphy et al 1992a). Later, high doses of GH was considered to contribute to the hematopoietic function in mice and rats after chemotherapy, radiotherapy and bone marrow transplantation (Carlo-Stella et al 2004; Chen et al 2010; Murphy et al 1992b; Tian et al 1998; Zhang et al 2008). Also, recombinant human GH (rhGH) could promote the recovery of platelets after chemotherapy (Sirohi et al 2007).…”