Effects of Human Pancreatic Tumor Growth Hormone-Releasing Hormone (hpGRHl-44-NH2) on Immunoreactive and Bioactive Plasma Growth Hormone in Normal Young Men*

Sassolas, G.; Chatelain, Pierre; Cohen, Richard A.; Boissel, Jean; Laporte, S; Galleyrand, Jacques; Bruno, Claudio; Elmcharfi, Abdel; Chayvialle, Jean‐Alain; Cohen, H.; Ferry, Serge; Underwood, Louis E.

doi:10.1210/jcem-59-4-705

Cited by 55 publications

(34 citation statements)

References 17 publications

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“…The serum samples were incubated for 18-24 h at 5G3 8C with PRL labelled with 125 I (20 000 cpm/ sample) and in-house rabbit anti-human PRL antibodies (27). These polyclonal antibodies were non-specific for the monomeric form of PRL (28).…”

Section: Assay Methodsmentioning

confidence: 99%

Macroprolactinaemia: a biological diagnostic strategy from the study of 222 patients

Parlant-Pinet

Harthé

Roucher

et al. 2015

European Journal of Endocrinology

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Objectives: Gel filtration chromatography (GFC), the gold standard for macroprolactinaemia (MPRL) diagnosis, is a slow, costly and labour-intensive method. To limit the number of GFC required, we evaluated two screening tests for MPRL: prolactin (PRL) recovery after polyethylene glycol (PEG) precipitation and PRL concentration ratio, derived from two assays, each having different big-big-PRL cross-reactivities. In some patients, MPRL is characterised by clinical symptoms which can be associated with an excess of monomeric PRL. We compared the monomeric PRL concentration obtained from GFC with the PRL concentration i) on a cobas e 601 analyser and ii) in the supernatant after PEG precipitation. Design and methods: We studied hyperprolactinaemic sera subjected to physician-ordered GFC, between February 2013 and July 2014. We performed PEG precipitation (to evaluate the PRL concentration and rate of recovery in the supernatant) and two PRL assays: RIA and electrochemiluminescent assay (ECLIA), on a Roche cobas e 601 analyser, and calculated the RIA/ECLIA ratio. Results: Among the 222 sera, we were able to diagnose or exclude MPRL in 72.1% of cases, based solely on the ratio and/or recovery. In the remaining cases, GFC was necessary for making a diagnosis. Elevated monomeric PRL was present in 10.9% of macroprolactinaemic sera. In the case of MPRL, both PRL measurements on the cobas analyser and in the supernatant weakly correlated with monomeric PRL values obtained from GFC. Conclusions: The combination of PEG and RIA/ECLIA ratio analysis reduced the number of necessary GFC. However, GFC is essential in MPRL cases to evaluate the monomeric PRL concentration.

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Section: Assay Methodsmentioning

confidence: 99%

Macroprolactinaemia: a biological diagnostic strategy from the study of 222 patients

Parlant-Pinet

Harthé

Roucher

et al. 2015

European Journal of Endocrinology

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“…While these cells might represent trapped normal lactotrophs, their abundance and the clinical observation of a high frequency of hyperprolactinaemia in patients with the ectopic GHRH syndrome (Losa et al, 1993) raise the possibility that chronic exposure to GHRH may also stimulate lactotroph growth. In fact, it has been demonstrated that normal subjects show a rise in PRL after the intravenous administration of larger doses (600 mg) of GHRH (Sassolas et al, 1984). Furthermore, mice transgenic for hGHRH have an increased number of lactotrophs and mammosomatotrophs (Stefaneanu et al, 1989).…”

Section: Morphology Of the Pituitarymentioning

confidence: 99%

Pathophysiology and clinical aspects of the ectopic GH‐releasing hormone syndrome

Losa¹,

Werder²

1997

Clinical Endocrinology

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“…Additionally, GABAB receptor stimulation may also have a stimulatory effect on serotonin and PRL release (22). Moreover, GHRH increases GH and PRL secretion (41). It is conceivable that SXB stimulates GHRH release and thereby promotes both GH and PRL.…”

Section: Discussionmentioning

confidence: 99%

Sodium oxybate increases prolactin secretion in narcolepsy patients and healthy controls

Donjacour

Aziz²,

Frölich³

et al. 2011

European Journal of Endocrinology

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Objective: Hypocretin deficiency causes narcolepsy and may affect neuroendocrine systems, including TSH, ACTH and LH secretion. Symptoms can be treated effectively with sodium oxybate (SXB) in many patients. This study was performed to compare prolactin (PRL) secretion in patients and matched controls and establish the effect of SXB administration on PRL and sleep in both the groups. Design: Open label intervention. Blood was sampled before and after 5 days of SXB treatment. The study was performed at the Leiden University Medical Centre, Leiden, The Netherlands. Methods: Subjects were admitted to the clinical research centre on both occasions. Patients or participants: Eight male hypocretin-deficient narcolepsy with cataplexy patients and eight controls matched for sex, age, body mass index, waist-to-hip ratio and fat percentage were enrolled. Interventions: SXB two times 3 g per night for five consecutive nights. Results: Patients and controls underwent 24 h blood sampling at 10 min intervals for measurement of PRL concentrations. The PRL concentration time series was analysed with a new deconvolution programme, approximate entropy (ApEn) and Cosinor analysis. Sleep was polygraphically recorded. Basal and pulsatile PRL secretion, as well as pulse regularity and frequency, ApEn and diurnal parameters were similar in patients and controls. SXB treatment caused similar nocturnal increase in PRL secretion, advance of the acrophase and decrease in ApEn in patients and controls. Slow wave sleep was increased to a similar extent in patients and controls. Conclusion: This detailed study did not demonstrate altered PRL secretion in hypocretin-deficient narcolepsy patients during the basal state or during SXB administration. Therefore, hypocretin signalling is unlikely to be a regulator of the lactotrophic system.

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Effects of Human Pancreatic Tumor Growth Hormone-Releasing Hormone (hpGRHl-44-NH2) on Immunoreactive and Bioactive Plasma Growth Hormone in Normal Young Men*

Cited by 55 publications

References 17 publications

Macroprolactinaemia: a biological diagnostic strategy from the study of 222 patients

Macroprolactinaemia: a biological diagnostic strategy from the study of 222 patients

Pathophysiology and clinical aspects of the ectopic GH‐releasing hormone syndrome

Sodium oxybate increases prolactin secretion in narcolepsy patients and healthy controls

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