Effects of human umbilical cord mesenchymal stem cells on co‐cultured ovarian carcinoma cells

Abstract

Ovarian carcinoma is mainly treated by surgery aided by chemotherapy. If supplemented by stem cells treatment, its recurrence rate and mortality rate will be decreased. This is a new therapy. In this study, ovarian cancer cells were cultured together with umbilical cord mesenchymal stem cells (UCMSCs), and the interactions between them were observed. The results showed that the survival rates of UCMSCs increased to 83.8 ± 2.2% from 56.5 ± 5.5%, and the survival rates of ovarian cancer cells decreased to 16.2 ± 2.2% from 43.5 ± 5.5% with the progression of the cultural time from 24 to 96 hr. There was a significant difference between them (p < .05). It revealed that UCMSCs could inhibit the proliferation of ovarian cancer cells.

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

“…The mortality rates of ovarian cancer are increasing and no known therapy has proven efficacy in inhibiting or delaying the progression of the disease. Recently MSC has emerged as a potential therapeutic cell therapy for many diseases including some form of cancer [32, 33]. Many controversies have been reported concerning the use of MSCs in cancer, few reporting the inhibition of the tumor invasiveness and metastasis [34, 35] and others reporting an increase in tumorogenecity [36, 37].…”

“…For example, UCMSCs displayed the obvious inhibition effects on tumorous growth in the research of malignant tumors including, melanoma, colon carcinoma, hepatocarcinoma, mammary cancer, hematological malignancy and pulmonary carcinoma [37–40] Xiufeng Li et al, showed that, with the prolonging of co-culture time of Caov-3 and hUCMSCs, the number of apoptotic CAOV3 cells became more and more; and their proliferation was inhibited remarkably where stem cell grew, ovarian cancer cells were enclosed first, then gradually necrosed and developed into clusters of granules [33]. This confirm that MSCs can alter the metastatic profile of ovarian cancer [33, 41].…”

“…The array of secretion or secretome have multiple therapeutic effects such as anti-inflammatory, anti-proliferative and anti-fibrotic effect beside many other roles [49–51]. The effects of the secretome has shown as anti-proliferative by many investigators such as Kalamegan et al, where they showed that Human Wharton’s Jelly Stem Cell (hWJSC) extracts inhibit ovarian cancer cell lines OVCAR3 and SKOV3 in vitro by inducing cell cycle arrest and apoptosis [52], in another study UCMSCs secretome have showed to significantly inhibit the proliferation of Skov-3 cells in addition the survival rates of ovarian cancer cells decreased with the exposure time of UCMSCs culture supernatant ie secretome [31, 33].…”

Human umbilical cord mesenchymal stem cell‐derived extracellular vesicles: A novel therapeutic paradigm

“…Ding, Chang, Shyu, & Lin, ; S. Yin, Ji, Wu, Jin, & Qian, ). Numerous studies have explored the potential effects of hucMSCs in treatment of various diseases such as diabetes (Bi et al, ), cancer (Xiufeng Li & Li, ), and liver (Guo et al, ), bone (Day et al, ), cartilage (K.‐C. Wu, Chang, Liu, & Ding, ), brain (Tanaka et al, ), and cardiovascular (X. Li et al, ) disorders both in vitro and in vivo.…”

The Dual Role of Mesenchymal Stromal Cells and Their Extracellular Vesicles in Carcinogenesis

“…At the same time, the synthesis of kinases, including AKT, phosphoinositide 3-kinase (PI3K), ERK, STAT3, and the mammalian target of rapamycin (mTOR), were significantly reduced in both tumor cell types when co-cultivated with MSCs [ 61 , 62 ]. Cord blood MSCs have also shown an inhibitory effect on ovarian cancer cells CAOV-3 in co-culture, where increases in the number of apoptotic CAOV-3 cells alongside decreased proliferation were noted [ 63 ].…”