Effects of humic acids on the growth of bacteria

Tikhonov, V. V.; Yakushev, A. V.; Zavgorodnyaya, Yu. A.; Byzov, B. A.; Демин, В. В.

doi:10.1134/s1064229310030087

Cited by 79 publications

(43 citation statements)

References 14 publications

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“…In this study, three bacteria Bacillus licheniformis, Microbacterium resistens and Stenotrophomonas maltophilia (both individual and combination) were selected to further investigate their HA degradation characteristics in arable soil on the basis of their growth performance and microbial biomass in HA solid medium. Our present work differs from previous researches in that that arable soil HA was used as the sole C and N source in the culture medium, whereas in other studies individual substances were often used as supplementary C (e.g., glucose) (Kontchou, Blondeau, 1992;Tikhonov et al, 2010) and N (e.g., yeast extract powder, ammonium tartrate and ammonium nitrate, NH 4 NO 3 ) sources in the culture medium. Combined analysis adopting infrared (IR) spectroscopy was to determine the effect of the HA-degrading bacteria on the HA degradation level and structural characteristics during the degradation process.…”

Section: Introductioncontrasting

confidence: 50%

Degradation capacity of humic acids-degrading bacteria on humic acids extracted from arable soil

Fu¹,

Liu²,

Chang³

et al. 2017

Zemdirbyste-Agriculture

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The aim of the present experiment was to determine the degradation level of humic acids (HA) extraced from arable soil by three selected strains. A short-term experiment was carried out in 2014 at State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University. HA was extracted from arable soil and was used as the sole carbon (C) and nitrogen (N) source in solid culture medium. Three soil HA-degrading strainsBacillus licheniformis (strain 1), Microbacterium resistens (strain 2) and Stenotrophomonas maltophilia (strain 3), were chosen to further investigate their individual and combined effects on the C degradation rate and structural properties of humic acids in the medium. The experiment included seven treatments: T1, T2, T3, T1+T2, T1+T3, T2+T3 and T1+T2+T3. Results showed that the total colony-forming units (CFU) of S. maltophilia (strain 3) showed higher amount in the treatments it was associated with. Carbon respiration (CR) and microbial biomass carbon (MBC) in various treatments varied between 4.04 (T2) to 12.13 (T2+T3) mg dish -1 and 1.32 (T2) to 4.96 (T3) mg dish -1 , in terms of the C degradation rates in humic acids, the sum of CR and MBC, followed the trend T2+T3 (52.05%) > T1+T3 (49.29%) > T1 (44.25%) > T3 (40.61%) > T1+T2 (38.39%) > T1+T2+T3 (37.09%) > T2 (18.91%). Infrared spectroscopy revealed that medium of all treatments resulted in higher levels of carbohydrate, aliphatic compounds, alcohols and nitrogenous substances after degradation process. T1 exhibited the lowest aromatic C content compared with the content before degradation, while that in T1+T2+T3 was the highest. Thus, B. licheniformis (strain 1) might have a relatively strong degradation capacity for aromatic C.

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Section: Introductioncontrasting

confidence: 50%

Degradation capacity of humic acids-degrading bacteria on humic acids extracted from arable soil

Fu¹,

Liu²,

Chang³

et al. 2017

Zemdirbyste-Agriculture

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“…From these results, we confirmed that the effects of niches and technological parameters on microbial community structure was significant. The texture and physicochemical properties of PM differed from those of ZP and HS, which contained humic acid that had a buffering capacity, and inhibited or stimulated some microbes [45]. Furthermore, the differences among the interphase samples indicated that transfer of microbes from PM to ZP was slow, but from ZP to HS or from PM to HS was more rapid.…”

Section: Discussionmentioning

confidence: 96%

Characterization of Interphase Microbial Community in Luzhou-Flavored Liquor Manufacturing Pits of Various Ages by Polyphasic Detection Methods

Huang

Liu³

et al. 2017

Journal of Microbiology and Biotechnology

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It is vital to understand the changing characteristics of interphase microbial communities and interspecies synergism during the fermentation of Chinese liquors. In this study, microbial communities in the three indispensable phases (pit mud, zaopei, and huangshui) of Luzhou-flavored liquor manufacturing pits and their shifts during cellars use were first investigated by polyphasic culture-independent approaches. The archaeal and eubacterial communities in the three phases were quantitatively assessed by combined phospholipid ether lipids/phospholipid fatty acid analysis and fluorescence in situ hybridization. In addition, qualitative information regarding the microbial community was analyzed by PCR-denaturing gradient gel electrophoresis. Results suggested that the interphase microbial community profiles were quite different, and the proportions of specific microbial groups evolved gradually. Anaerobic bacteria and gram-positive bacteria were dominant and their numbers were higher in pit mud (10 cells/g) than in huangshui (10 cells/ml) and zaopei (10cells/g). Hydrogenotrophic methanogenic archaea were the dominant archaea, and their proportions were virtually unchanged in pit mud (around 65%), whereas they first increased and then decreased in zaopei (59%-82%-47%) and increased with pit age in huangshui (82%-92%). Interactions between microbial communities, especially between eubacteria and methanogens, played a key role in the formation of favorable niches for liquor fermentation. Furthermore, daqu (an essential saccharifying and fermentative agent) and metabolic regulation parameters greatly affected the microbial community.

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“…Interestingly, the highest relative abundance of lactobacilli was observed in the HNa + ZnO group at this sampling time. It is noteworthy in this regard that HS may influence metabolism in microbes and can be a source of nutrients for some microorganisms with subsequent stimulation of their growth (Tikhonov et al 2010). Shermer et al (1998) observed that dietary HS (0-50 g/kg feed) may promote higher Lactobacillus population levels in the caecal bacterial flora of broilers.…”

Section: Discussionmentioning

confidence: 99%

Effect of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on faecal microbiota composition in weaned piglets

Kaevska¹,

Lorencova²,

Vídeňská³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to use high throughput sequencing of piglet faeces to investigate if the favourable effects of sodium humate (HNa) and zinc oxide (ZnO) in post-weaning diarrhoea (PWD) treatment are related to changes in the faecal microbiota. Twenty four piglets weaned at 28 days of age were divided into three groups with eight animals per pen: a control group without any treatment (Control), a group treated with 2500 mg ZnO (ZnO), and a group treated with 20 g sodium humate and 1700 mg ZnO (HNa + ZnO) per kg of diet. Piglets of all three groups were challenged with two enterotoxigenic Escherichia coli (ETEC) strains (ETEC/O149/F4/LT and ETEC/O147/F18/LT) on Day 4 post-weaning. As a result very intense and severe diarrhoea with high mortality developed in the ETEC-infected control group, while the ZnO and HNa + ZnO dietary treatments both protected piglets from clinical signs of diarrhoea, mortality and depression of growth performance. A higher relative abundance of Gammaproteobacteria represented mainly by genus Escherichia on Day 10 postweaning in faeces of the ETEC-infected control group in comparison with ZnO and HNa + ZnO was detected. On Day 21, the highest relative increase of beneficial lactobacilli was observed in the HNa + ZnO group. Correlation analysis showed a positive correlation of the ETEC-infected control with the genera Turicibacter, Clostridium, Campylobacter, Dehalobacterium, Desulfuvibrio, Paludibacter and a negative correlation with the genera Prevotella, Blautia, Faecalibacterium, Lactobacillus, and Coprococcus. The opposite correlations with these genera were observed in the supplemented groups, especially in the HNa + ZnO group. The results indicate that dietary supplementation with HNa and ZnO affects the microbial composition of faeces while maintaining good health condition and growth performance of ETEC-infected weaned pigs.

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Effects of humic acids on the growth of bacteria

Cited by 79 publications

References 14 publications

Degradation capacity of humic acids-degrading bacteria on humic acids extracted from arable soil

Degradation capacity of humic acids-degrading bacteria on humic acids extracted from arable soil

Characterization of Interphase Microbial Community in Luzhou-Flavored Liquor Manufacturing Pits of Various Ages by Polyphasic Detection Methods

Effect of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on faecal microbiota composition in weaned piglets

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