Effects of Hyaluronidase during <i>In Vitro</i> Maturation on Maturation and Developmental Competence in Porcine Oocytes

Jeon, Ye-Eun; Hwangbo, Yong; Cheong, Hyeonsook; Park, Choon‐Keun

doi:10.12750/jarb.34.2.86

Cited by 4 publications

(4 citation statements)

References 20 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the average CC expansion size in the control group, grades were divided into 0–200, 200–300, 300–400, and > 400 μm. Both the control and treatment groups were separated according to these criteria and the ratios were compared [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Sun

Jiang

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex Ⅰ. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information on the effects of rotenone exposure in porcine oocytes during in vitro maturation, and the mechanisms underlying these effects have not been determined. The Cumulus-oocyte complexes were supplemented with different concentrations of rotenone to elucidate the effects of rotenone exposure on the meiotic maturation of porcine oocytes during in vitro maturation for about 48 hours. First, we found that the maturation rate and expansion of cumulus cells were significantly reduced in the 3 and 5 μM rotenone-treated groups. Subsequently, the concentration of rotenone was determined to be 3 μM. Also, immunofluorescence, western blotting, and image quantification analyses were performed to test the rotenone exposure on the meiotic maturation, total and mitochondrial ROS, mitochondrial function and biogenesis, mitophagy and apoptosis in porcine oocytes. Further experiments showed that rotenone treatment induced mitochondrial dysfunction and failure of mitochondrial biogenesis by repressing the level of SIRT1 during in vitro maturation of porcine oocytes. In addition, rotenone treatment reduced the ratio of active mitochondria to total mitochondria, increased ROS production, and decreased ATP production. The levels of LC3 and active-caspase 3 were significantly increased by rotenone treatment, indicating that mitochondrial dysfunction induced by rotenone increased mitophagy but eventually led to apoptosis. Collectively, these results suggest that rotenone interferes with porcine oocyte maturation by inhibiting mitochondrial function.

show abstract

Section: Methodsmentioning

confidence: 99%

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Sun

Jiang

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Considering the average CC expansion size in the control group, grades were divided into 0-200, 200-300, 300-400, and > 400 µm. Both the control and treatment groups were separated according to these criteria and the ratios were compared [35].…”

Section: Assessment Of Cumulus Cell Expansionmentioning

confidence: 99%

Rotenone Disrupts Meiotic Maturation via Inhibition of Mitochondrial Function in Porcine Oocytes

Heo

Sun

Jiang

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex 1. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information on the effects of rotenone exposure in porcine oocytes during in vitro maturation, and the mechanisms underlying these effects have not been determined.Results: First, we found that the maturation rate and expansion of cumulus cells was significantly reduced in the 3 and 5 μM rotenone-treated groups. Further experiments showed that rotenone treatment induced mitochondrial dysfunction and failure of mitochondrial biogenesis by repressing the levels of PGC1-α and SIRT1 during in vitro maturation of porcine oocytes. In addition, rotenone treatment reduced the ratio of active mitochondria to total mitochondria, increased ROS production, and decreased ATP production. The levels of LC3 and active-caspase 3 were significantly increased by rotenone treatment, indicating that mitochondrial dysfunction induced by rotenone increased mitophagy but eventually led to apoptosis.Conclusions: Collectively, these results suggest that rotenone interferes with porcine oocyte maturation by inhibiting mitochondrial function.

show abstract

“…Ovaries from Hanwoo cattle were collected from a local slaughterhouse, and transported in saline at 38.5 o C to the laboratory. In vitro oocyte maturation was performed as previously described (Hwang et al, 2016;Choudhury et al, 2017;Hassan et al, 2017;Choi et al, 2019;Jeon et al, 2019). Cumulus oocyte complexes were aspirated from the follicles using a syringe, washed three times in Tyrode's lactate-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, and cultured in tissue culture medium 199 (TCM 199) supplemented with 10% fetal calf serum (10082-147; Gibco, USA), 1 μg/mL β-estradiol (E2758; Sigma, USA), 10 μg/ mL follicle-stimulating hormone (F2293; Sigma), 10 ng/mL epidermal growth factor (E4127; Sigma), 100 IU/mL penicillin/streptomycin, 22 μg/mL sodium pyruvate (P5280; Sigma), and 95 μg/mL L-cysteine (C7352; Sigma) at 38.5 o C in a humidified atmosphere with 5% CO 2 and 95% air.…”

Section: Collection and In Vitro Maturation Of Cattle Oocytesmentioning

confidence: 99%

“…Total ROS levels in oocytes were determined using 2′, 7′-dichlorodihydrofluorescein diacetate (H 2 DCF-DA, Cat # D399, Molecular Probes, USA) as previously described (Nasr-Esfahani et al, 1990;Park et al, 2017;Fang et al, 2018;Jeon et al, 2019). Briefly, oocytes were incubated for 15 min in PBS/PVA containing 10 μM H 2 DCF-DA at 38.5 o C, and washed three times with PBS/PVA.…”

Section: Ros Measurementsmentioning

confidence: 99%

Nitric Oxide-induced Protein S-nitrosylation Causes Mitochondrial Dysfunction and Accelerates Post-ovulatory Aging of Oocytes in Cattle

Niu

Zhou

et al. 2020

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

Effects of Hyaluronidase during In Vitro Maturation on Maturation and Developmental Competence in Porcine Oocytes

Abstract: During the maturation process of mammalian oocytes, cumulus cells surrounding oocytes undergo morphological and physiological changes and these changes of cumulus cells directly influence to maturation and develop

Cited by 4 publications

References 20 publications

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Rotenone Disrupts Meiotic Maturation via Inhibition of Mitochondrial Function in Porcine Oocytes

Nitric Oxide-induced Protein S-nitrosylation Causes Mitochondrial Dysfunction and Accelerates Post-ovulatory Aging of Oocytes in Cattle

Contact Info

Product

Resources

About