Effects of Hydrocortisone and Heparin on Growth and Glycosaminoglycan Synthesis of Cultured Human Fibroblasts (WI-38)

Schachtschabel, D.O.; Sluke, G; Wever, J.

doi:10.1007/978-3-642-70788-9_5

Cited by 4 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could have occurred through the presence of Ca2+-binding metabolites such as glycosaminoglycans (GAGs), of which Vannucchi et al (1978) have shown sulfated GAGs to be particularly effective in binding Ca". GAGs have been found to be released into the medium by human embryonic lung-derived fibroblastic cell lines (Schachtschabel and Wever, 1978;Schachtschabel et al, 1979;Vogel et al, 1981;Matuoka and Mitsui, 1981).…”

Section: Discussionmentioning

confidence: 99%

Modulation of WI‐38 cell proliferation by elevated levels of CaCl₂

Praeger

Cristofalo

1986

Journal Cellular Physiology

View full text Add to dashboard Cite

Elevating the level of extracellular calcium (CaEx2+) increases the saturation density achieved by the normal human diploid cell line, WI-38, but does not change the growth rate. Day 7 cell yields remain unchanged when [CaEx2+] is between 0.5 mM and 3.0 mM, decrease when [CaEx2+] less than 0.5 mM, and increase when [CaEx2+] greater than 3.0 mM. Combining hydrocortisone with additional CaCl2 results in an additive effect on the saturation density relative to that obtained with each treatment separately. The stimulatory effect of elevated [CaCl2] is independent of serum concentration but is lost when WI-38 cells are grown in conditioned medium. Stimulation is recovered when conditioned medium is diluted with serum-free medium. In the case of young cultures grown in conditioned medium, stimulation can also be recovered when higher than usual levels of additional CaCl2 are used (2-3 mM). A glutamine supplementation to the conditioned medium potentiates cell response to elevated [CaCl2]. These results indicate that the loss of an enhanced saturation density when cells are grown in conditioned medium is not due to serum depletion but is more likely the effect of metabolites and/or nutrient depletion. When older or less vigorously growing cultures are grown in conditioned medium, additions of up to 3 mM CaCl2 only lead to inhibition, suggesting an age-related change in proliferative regulation. Elevated levels of CaEx2+ also enhance the proliferative response of quiescent monolayers to serum stimulation. This finding, along with the increase in saturation density of Ca2+-treated cultures, suggests that an elevated level of CaEx2+ affects cell entry into and exit from quiescence brought on by density-dependent inhibition.

show abstract

Section: Discussionmentioning

confidence: 99%