Effects of Hydrostatic Pressure Treatment of Newly Fertilized Eggs on the Ploidy Level and Karyotype of Pikeperch Sander lucioperca (Linnaeus, 1758)

Abstract

We studied the effect of different magnitudes (7000 PSI (48.26 MPa), 8000 PSI (55.16 MPa), and 9000 PSI (62.05 MPa)) of hydrostatic pressure on the ploidy of pikeperch larvae. Pressure shock was applied 5 min after the fertilization of eggs at a water temperature of 14.8 ± 1 °C. A 7000 PSI pressure shock was applied for 10 or 20 min, while 8000 and 9000 PSI treatments lasted for 10 min. Each treatment with its respective control was completed in triplicate, where different females’ eggs served as a replicate. In the treatment groups exposed to 7000 PSI for 10 min, only diploid and triploid larvae were identified, while 2n/3n mosaic individuals were found after a 20-min exposure to a 7000 PSI pressure shock. The application of 8000 or 9000 PSI pressure shocks resulted in only triploid and mosaic individuals. Among larvae from eggs treated with 8000 PSI, three mosaic individuals with 2n/3n karyotype were identified (4.0 ± 6.9%), while a single (2.0 ± 3.5%) 1n/3n mosaic individual was found in the 9000 PSI-treated group. To our knowledge, this is the first report that demonstrates the induction of a haplo-triploid karyotype by hydrostatic pressure shock in teleost fish. The dominance of triploid individuals with a reasonable survival rate (36.8 ± 26.1%) after 8000 PSI shock supports the suitability of the hydrostatic pressure treatment of freshly fertilized eggs for triploid induction in pikeperch.

Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based oncntd1Knockout in Zebrafish

“…This method of artificial induction yields triploid or tetraploid fish by interfering with processes like the extrusion of the second polar body or the initiation of the first zygotic mitosis. This approach has been explored in various species ( Peruzzi and Chatain 2003 ; Nam et al 2004 ; Hamasaki et al 2013 ; Goo et al 2015 ; Baars et al 2016 ; Zhu et al 2017 ; Hassan et al 2018 ; Glover et al 2020 ; Káldy et al 2021 ). Although stressors such as high or low temperatures and hydrostatic pressure can occur naturally, achieving the precise timing required for polyploid induction under natural conditions presents challenges and demands meticulous control.…”

Unidirectional hybridization between American paddlefish Polyodon spathula (Walbaum, 1792) and sterlet Acipenser ruthenus (Linnaeus, 1758)

“…Data were analyzed as previously described in Káldy et al (2021) . Specifically, R Studio Team (version: 2022.7.2.576) and R software (version: 4.3.1 ( R Core Team, 2023 )) were used to build a logistic regression model (generalized linear model) in which ‘fertilized eggs ratio’ was the response variable, and ‘parent combination’ was the predictor variable.…”