Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7)

Meena, Ramovatar; Kesari, Kavindra Kumar; Rani, Madhu; Paulraj, R

doi:10.1007/s11051-011-0712-5

Cited by 106 publications

(64 citation statements)

References 46 publications

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“…Like the comet test, the SCE, MN and CA methods are widely used to detect the chemicals’ potential to cause DNA damage (Turkez, ; Turkez and Geyikoglu, ; Turkez and Togar, ), and 8‐OH‐dG is widely accepted as a reliable biomarker of oxidative DNA damage (Turkez et al ., ). In accordance with our finding, HAP NPs induced the production of intracellular reactive oxygen species, which might be responsible for DNA damage in human breast cancer MCF‐7 cells (Meena et al ., ). On the contrary, Ding et al .…”

Section: Discussionmentioning

confidence: 97%

Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells

Türkez

Yousef

Sönmez

et al. 2013

J of Applied Toxicology

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The present study was designed to investigate genotoxic and cytotoxic effects and oxidative damage of increasing concentrations of nano-hydroxyapatite (5, 10, 20, 50, 75, 100, 150, 300, 500 and 1000 ppm) in primary human blood cell cultures. Cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] assay and lactate dehydrogenase release, while total antioxidant capacity and total oxidative stress levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by sister chromatid exchange, micronuclei, chromosome aberration assays and 8-oxo-2-deoxyguanosine level as indicators of genotoxicity. The results of [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] and lactate dehydrogenase assays showed that the higher concentrations (150, 300, 500 and 1000 ppm) of hydroxyapatite nanoparticles (HAP NPs) decreased cell viability. HAP NPs led to increases of total oxidative stress (300, 500 and 1000 ppm) levels and decreased total antioxidant capacity (150, 300, 500 and 1000 ppm) levels in cultured human blood cells. On the basis of increasing concentrations, HAP NPs caused significant increases of sister chromatid exchange, micronuclei, chromosome aberration rates and 8-oxo-2-deoxyguanosine levels as compared to untreated culture. In conclusion, the obtained in vitro results showed that HAP NPs had dose-dependent effects on inducing oxidative damage, genotoxicity and cytotoxicity in human blood cells.

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Section: Discussionmentioning

confidence: 97%

Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells

Türkez

Yousef

Sönmez

et al. 2013

J of Applied Toxicology

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“…Cytocompatibility assessment is the initial step for toxicology evaluation of any biomaterial . Previous studies have already reported the dose‐dependent cytotoxicity of nHA to various cell types via apoptotic/necrotic induction . The degree of cytotoxicity caused by nHA was correlated well with the degree of cellular uptake of nHA, which was probably attributed to the Ca 2+ release .…”

Section: Discussionmentioning

confidence: 97%

“…For instance, Müller et al have reported that nHA (∼30 nm) caused cytotoxicity to human monocyte‐derived macrophages at 31.25–500 μg/mL. An investigation conducted by Ramovatar et al showed that nHA (∼20 nm) inhibited the cellular proliferation of human breast cancer cells at 50–250 μg/mL in a dose‐dependent manner. While, Remya et al found that synthesized nHA (∼50 nm) did not induce cytotoxicity to mouse bone marrow mesenchymal stem cells even up to 800 μg/mL.…”

Section: Introductionmentioning

confidence: 99%

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

Yang

Huang

et al. 2018

J Biomedical Materials Res

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Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity; however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized, and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis, and the expression of adipogenic marker genes/proteins of hMSCs at concentrations lower than 50 μg/mL. It is concluded that the adipogenic differentiation potential of hMSCs is not affected by nHA at noncytotoxic concentrations. These will provide a reference for the applications of nHA in biomedical fields. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1822-1831, 2018.

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“…Research suggests that the inhibitory of HA had dose-dependent manner, which was better with the increasing of HA concentration. However, another paper points out that with the increasing of HA concentration, it not only inhabited the hepatocarcinoma cell, but also caused damage to hepatocyte [16].…”

Section: Introductionmentioning

confidence: 99%

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

Chen¹,

Xu²,

Wang³

et al. 2017

Proceedings of the 2017 International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2017)

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Abstract. The exploration of the apoptosis for HepG2 cells induced by low-frequency and low-power ultrasound combined with hydroxyapatite, provides data base for future clinical treatment. In this research, we selected the HepG2 cells model. The experiences were divided into four groups: control group, ultrasound group, hydroxyapatite group, and ultrasound + hydroxyapatite group. Ultrasound group and control group replaced the normal culture medium, hydroxyapatite group and ultrasound + hydroxyapatite group replaced culture medium containing hydroxyapatite. The control group and hydroxyapatite group were not treated, while ultrasound group and ultrasound + hydroxyapatite group were treated by ultrasound with different parameters (f= 20 kHz; P=0.1, 0.2, 0.3w/cm 2 ; t=30, 60, 90, 120, 150, 180s; T= 0, 12, 24, 48, 72h). MTT test and flow cytometry detected the inhibitory rate of the cells, cell apoptosis and cell cycle. MTT experiment results show that ultrasound with different parameters stimulates the cell model, which leads to the difference of the inhibition rate of cell growth. Among them, the inhibition rate of cell growth reached the top value (71.726±0.677)% with the treatment of f=20 kHz, P =0.2w/cm 2 ,t=90s,T=48h. There was a statistical significance compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05).Under such condition, the apoptosis rate of HepG2 cells was a statistically significant change compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05). During the cell cycle assay, the ultrasonic + hydroxyapatite group also showed obvious G2/M phase block. Low frequency and low-power ultrasound with Hydroxyapatite induced the apoptosis in human HepG2 cells.

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Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7)

Cited by 106 publications

References 46 publications

Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells

Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

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