Effects of hydroxylated polychlorinated biphenyls on mouse liver mitochondrial oxidative phosphorylation

Narasimhan, T.R.; Kim, H L; Safe, Stephen

doi:10.1002/jbt.2570060309

Cited by 38 publications

(23 citation statements)

References 16 publications

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“…PCBs have been found to accumulate in certain animal cells causing the loss of cell viability and accelerating apoptosis by disrupting mitochondrial functions [3][4][5][6][7][8][9][12][13][14][15][16]. Recent studies clarified that mitochondrial MPT plays an important role in the mechanism of apoptosis [23].…”

Section: Discussionmentioning

confidence: 99%

“…As described previously, various PCBs disrupt the biochemical functions of mitochondria [12][13][14][15][16][17][18]. To help understand the process by which such disruption occurs, we examined the effect of 4-OH-TCB on mitochondrial respiration and oxidative phosphorylation.…”

Section: Uncoupling Of Mitochondrial Oxidative Phosphorylation By 4-omentioning

confidence: 99%

“…In preliminary experiment of this laboratory, we observed that 4-hydroxy-3,5,3',4'-tetrachlorobiphenyl (4-OH-TCB) increased thyroxin-induced tail shortening in tadpoles in a manner typical of apoptosis at organ level. Several investigations indicate that biological membranes might be the sites for the toxic action of PCBs [10][11][12][13][14][15][16][17][18][19]. Studies of the action of chlorinated hydrocarbon pesticides on mitochondria indicate that PCBs may affect the electron transport and energy transducing reaction of rat liver mitochondria [12][13][14][15][16]20].…”

Section: Introductionmentioning

confidence: 99%

“…Several investigations indicate that biological membranes might be the sites for the toxic action of PCBs [10][11][12][13][14][15][16][17][18][19]. Studies of the action of chlorinated hydrocarbon pesticides on mitochondria indicate that PCBs may affect the electron transport and energy transducing reaction of rat liver mitochondria [12][13][14][15][16]20]. PCBs inhibited oxidative phosphorylation [12,16], decreased respiratory control [2,15] and inhibited oligomycin sensitive ATPase [17,21] in rat liver mitochondria.…”

Section: Introductionmentioning

confidence: 99%

“…Studies of the action of chlorinated hydrocarbon pesticides on mitochondria indicate that PCBs may affect the electron transport and energy transducing reaction of rat liver mitochondria [12][13][14][15][16]20]. PCBs inhibited oxidative phosphorylation [12,16], decreased respiratory control [2,15] and inhibited oligomycin sensitive ATPase [17,21] in rat liver mitochondria. Kanechlor-400 also inhibits succinic dehydrogenase and the CoQ-cytochrome c region of the electron transport chain [14].…”

Section: Introductionmentioning

confidence: 99%

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4-Hydroxy-3,5,3',4'-Tetrachlorobiphenyl Induced Membrane Permeability Transition in Isolated Rat Liver Mitochondria

Fujita

Okimura

Utsumi

et al. 2006

J. Clin. Biochem. Nutr.

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Summary Although much information about the apoptotic and other detrimental actions of polychlorinated biphenyls (PCBs) has been accumulated, the critical role of mitochondrial damage in such toxic action remains to be clarified. We have previously shown that PCBs affect various functions in isolated mitochondria. Recent studies have revealed that mitochondria play an important role in apoptosis through membrane permeability transition (MPT), and that Ca 2+ induces classic MPT characterized by depolarization and swelling of the mitochondria, thereby releasing cytochrome c in a cyclosporin A-sensitive mechanism [Free Rad. Res. 38, 29-35, 2004]. In the present study we investigated the effect of 4-hydroxy-3,5,3',4'-tetrachlorobiphenyl (4-OH-TCB) on isolated rat liver mitochondria in order to help clarify the effect of PCBs on MPT. Biochemical analysis revealed that 4-OH-TCB induced calcium release, reactive oxygen species (ROS) generation, depolarization, swelling and cytochrome c release in isolated rat liver mitochondria in a time and concentration dependent manner. These 4-OH-TCB-induced changes in mitochondrial function were found to be inhibited by the presence of cyclosporin A, suggesting an classic type of MPT. It is concluded that 4-OH-TCB induces MPT together with ROS generation, causing apoptosis in certain cells.

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Section: Discussionmentioning

confidence: 99%

Section: Uncoupling Of Mitochondrial Oxidative Phosphorylation By 4-omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

4-Hydroxy-3,5,3',4'-Tetrachlorobiphenyl Induced Membrane Permeability Transition in Isolated Rat Liver Mitochondria

Fujita

Okimura

Utsumi

et al. 2006

J. Clin. Biochem. Nutr.

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Enantioselective semipreparative HPLC separation of PCB metabolites and their absolute structure elucidation using electronic and vibrational circular dichroism

Pham-Tuan¹,

Larsson²,

Hoffmann³

et al. 2005

Chirality

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Polychlorinated biphenyls (PCBs) remain one of the most important groups of environmental contaminants. The fate (transformation) as well as the toxicological implications of the different metabolism steps are subject to considerable debate. The aim of this study is to start a comprehensive investigation of atropisomeric PCB metabolites, i.e., hydroxy, methoxy, methylthionyl, and methylsulfonyl PCBs in different biota. For this purpose, enantioselective semipreparative liquid chromatography is used to obtain pure enantiomers of PCB metabolites. Electronic circular dichroism (UV-CD) and vibrational circular dichroism (VCD) in combination with computational techniques were applied to determine their absolute structures. Approximately 18-25 mg of each enantiomer of the following metabolites were obtained using semipreparative HPLC on beta-cyclodextrin-based columns: 4-MeO-CB149, 4-MeS-CB149, 4-MeSO2-CB149, 3-MeS-CB149, and 3-MeSO2-CB149. The enantiomeric purity of the separated enantiomers was in the range of 95.0-99.9%. Rotational angles and absolute configurations were also determined. This study establishes a sound method for future preparation and absolute structure determination of compounds belonging to the same class.

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Polychlorinated biphenyls in prospectively collected serum and Parkinson's disease risk

et al. 2012

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Background Evidence suggests possible Parkinson’s disease (PD)-relevant neural effects of exposure to polychlorinated biphenyls. Limited epidemiological evidence suggests that polychlorinated biphenyl exposure may increase PD risk, but no studies have involved biomarkers of polychlorinated biphenyl exposure before PD onset. We examined the prospective association between serum polychlorinated biphenyls and PD. Methods We conducted a nested case-control study within the Finnish Mobile Clinic Health Examination Survey, with serum samples collected during 1968–1972, and analyzed in 2005–2007 for polychlorinated biphenyls. Incident PD cases were identified through the Social Insurance Institution’s registry and confirmed by medical record review (n=101). Controls (n=349) were matched on age, sex, municipality, and vital status. We used logistic regression to estimate adjusted odds ratios. Results There was no evidence of increasing risk of PD with increasing polychlorinated biphenyl exposure in adjusted analyses. Instead, there was a trend toward lower odds of PD with increasing serum polychlorinated biphenyl concentrations, which was most pronounced for the sum of all measured polychlorinated biphenyl congeners and the sum of dioxin-like congeners. Compared with those in the lowest quintile, the odds ratio of PD among those in the highest quintile of total polychlorinated biphenyls was 0.29 (95% confidence interval: 0.12–0.70; p-trend=0.02) and for dioxin-like congeners was 0.34 (95% confidence interval: 0.13–0.90; p-trend=0.05). Conclusions These results do not support an increased risk of PD from polychlorinated biphenyl exposure, and instead suggest a possible protective effect of polychlorinated biphenyl exposure. Our findings may have implications for PD neuropathology, although alternative explanations should be considered.

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Effects of hydroxylated polychlorinated biphenyls on mouse liver mitochondrial oxidative phosphorylation

Cited by 38 publications

References 16 publications

4-Hydroxy-3,5,3',4'-Tetrachlorobiphenyl Induced Membrane Permeability Transition in Isolated Rat Liver Mitochondria

4-Hydroxy-3,5,3',4'-Tetrachlorobiphenyl Induced Membrane Permeability Transition in Isolated Rat Liver Mitochondria

Enantioselective semipreparative HPLC separation of PCB metabolites and their absolute structure elucidation using electronic and vibrational circular dichroism

Polychlorinated biphenyls in prospectively collected serum and Parkinson's disease risk

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