Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901

Qi, Yinliang; Ruan, Jianfeng; Wang, Mei; Dai, Yuan‐Chang; Zhou, Qing; Gui, Shuyu; Wang, Yuan

doi:10.3892/br.2017.869

Cited by 10 publications

(8 citation statements)

References 36 publications

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“…Surprisingly, CHOP and GRP78 levels were decreased when gastric cancer cells were exposed to HBO, but treatment with HBO combined with MLT resulted in significant increases in levels of GRP78 and CHOP (Figure ). This is consistent with the finding that HBO itself does not induce cells apoptosis, and may even have the opposite effect . This further confirmed that hyperbaric oxygen alone is not suitable for use in cancer therapy.…”

Section: Discussionsupporting

confidence: 91%

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Hyperbaric oxygen treatment sensitizes gastric cancer cells to melatonin‐induced apoptosis through multiple pathways

Wei

Jia

et al. 2018

J of Cellular Biochemistry

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Although extensive efforts have been made in recent decades to treat advanced gastric cancer with comprehensive therapy based on chemotherapy, effective anti-gastric cancer therapeutics are still lacking in the clinics. Therefore, potent novel anti-gastric cancer ways are greatly needed. Here, we explored hyperbaric oxygen treatment as a novel and effective adjuvant treatment method which has anti-gastric cancer effects when used together with melatonin. When performed together with MLT, HBO effectively inhibited tumorigenicity of gastric cancer through selectively inducing a robust tumor suppressive apoptosis response. Mechanistic studies revealed that the sensitizing effect of hyperbaric oxygen is due to decreased ratio of Bcl-2/Bax, increased level of p53, cleaved Caspase3, GRP78, CHOP, and LC3. These results give a vivid picture that classic apoptosis pathways including mitochondrial pathway, tumor suppressive endoplasmic reticulum stress (ERS), and autophagy are all involved in the process. From the preliminary results got from the current study, we identified that HBO sensitizes human gastric cancer cells to MLT-induced apoptosis through a variety of complicated molecular mechanisms. HBO may provide a novel candidate supplemental treatment method for further development of potential anti-gastric cancer therapeutics. The combination of HBO and MLT could be a promising treatment for advanced gastric cancer.

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Section: Discussionsupporting

confidence: 91%

“…This is consistent with the finding that HBO itself does not induce cells apoptosis, and may even have the opposite effect. 42 This further confirmed that hyperbaric oxygen alone is not suitable for use in cancer therapy. HBO acts as a therapeutic sensitizer so as to play an anti-tumor role.…”

Section: Discussionmentioning

confidence: 68%

Hyperbaric oxygen treatment sensitizes gastric cancer cells to melatonin‐induced apoptosis through multiple pathways

Wei

Jia

et al. 2018

J of Cellular Biochemistry

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“…Furthermore, the expression of miR-573 was decreased whereas that of Bax was increased oppositely in human IDD tissues [15]. HBO treatment upregulates the ratio of Bcl-2 to Bax expression and reduced the apoptosis of ischemic tissue [27,28] or degenerated NPCs [29]. In this study, we further found the epigenetic regulations of Bax mRNA expression.…”

Section: Discussionsupporting

confidence: 56%

Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment

Lin

Niu

Yuan

et al. 2020

Preprint

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Background: MicroRNA (miRNA) plays a vital role in the intervertebral disc (IVD) degeneration. The expression level of miR-573 was downregulated whereas Bax was upregulated notably in human degenerative nucleus pulposus cells (NPCs). In this study, we aimed to investigate the role of miR-573 in human degenerative NPCs following hyperbaric oxygen (HBO) treatment. Methods: NPCs were separated from human degenerated IVD tissues. The control cells were maintained in 5% CO2/95% air and the hyperoxic cells were exposed to 100% O2 at 2.5 atmospheres absolute. MiRNA expression profiling was performed via microarray and confirmed by real-time PCR, and miRNA target genes were identified using bioinformatics and luciferase reporter assays. The mRNA and protein levels of Bax were measured. The proliferation of NPCs were detected using MTT assay. The protein expression levels of Bax, cleaved caspase 9, cleaved caspase 3, pro-caspase 9 and pro-caspase 3 were examined.Results: Bioinformatics analysis indicated that the 3′ untranslated region (UTR) of the Bax mRNA contained the “seed-matched-sequence” for hsa-miR-573, which was validated via reporter assays. MiR-573 was induced by HBO and simultaneous suppression of Bax was observed in NPCs. Knockdown of miR-573 resulted in upregulation of Bax expression in HBO-treated cells. In addition, overexpression of miR-573 by HBO increased cell proliferation and coupled with inhibition of cell apoptosis. The cleavage of pro‑caspase 9 and pro‑caspase 3 was suppressed while the levels of cleaved caspase 9 and caspase 3 were decreased in HBO-treated cells. Transfection with anti-miR-573 partly suppressed the effects of HBO. Conclusion: Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative NPCs following HBO treatment.

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“…Furthermore, the expression of miR-573 was decreased whereas that of Bax was increased oppositely in human IDD tissues [ 15 ]. HBO treatment upregulates the ratio of Bcl-2 to Bax expression and reduced the apoptosis of ischemic tissue [ 32 , 33 ] or degenerated NP cells [ 34 ]. In this study, we further found the epigenetic regulations of Bax mRNA expression.…”

Section: Discussionmentioning

confidence: 99%

Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment

et al. 2021

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Background MicroRNA (miRNA) plays a vital role in the intervertebral disc (IVD) degeneration. The expression level of miR-573 was downregulated whereas Bax was upregulated notably in human degenerative nucleus pulposus cells. In this study, we aimed to investigate the role of miR-573 in human degenerative nucleus pulposus (NP) cells following hyperbaric oxygen (HBO) treatment. Methods NP cells were separated from human degenerated IVD tissues. The control cells were maintained in 5% CO2/95% air and the hyperoxic cells were exposed to 100% O2 at 2.5 atmospheres absolute. MiRNA expression profiling was performed via microarray and confirmed by real-time PCR, and miRNA target genes were identified using bioinformatics and luciferase reporter assays. The mRNA and protein levels of Bax were measured. The proliferation of NPCs was detected using MTT assay. The protein expression levels of Bax, cleaved caspase 9, cleaved caspase 3, pro-caspase 9, and pro-caspase 3 were examined. Results Bioinformatics analysis indicated that the 3′ untranslated region (UTR) of the Bax mRNA contained the “seed-matched-sequence” for hsa-miR-573, which was validated via reporter assays. MiR-573 was induced by HBO and simultaneous suppression of Bax was observed in NP cells. Knockdown of miR-573 resulted in upregulation of Bax expression in HBO-treated cells. In addition, overexpression of miR-573 by HBO increased cell proliferation and coupled with inhibition of cell apoptosis. The cleavage of pro-caspase 9 and pro-caspase 3 was suppressed while the levels of cleaved caspase 9 and caspase 3 were decreased in HBO-treated cells. Transfection with anti-miR-573 partly suppressed the effects of HBO. Conclusion Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative NP cells following HBO treatment.

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Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901

Cited by 10 publications

References 36 publications

Hyperbaric oxygen treatment sensitizes gastric cancer cells to melatonin‐induced apoptosis through multiple pathways

Hyperbaric oxygen treatment sensitizes gastric cancer cells to melatonin‐induced apoptosis through multiple pathways

Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment

Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment

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